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Researcher Information

last modified:2019/04/10

Professor TAKINO Takahisa

Mail

Faculty, Affiliation

Institute of Liberal Arts and Science

College and School Educational Field

Division of Cancer Medicine, Graduate School of Medical Science
Division of Life Sciences, Graduate School of Natural Science and Technology

Laboratory

Molecular Virology and Oncology TEL:076-264-6712 FAX:

Academic Background

【Academic background(Doctoral/Master's Degree)】
Kanazawa University Doctor 1995 Completed
【Academic background(Bachelor's Degree)】
The University of Tokushima 199003

Career

Japan Society for Promotion of Science(1995/04/01-1997/03/31)
National Institutes of Health(1997/04/01-1997/12/31)
Japan Society for Promotion of Science(1998/01/01-1999/09/30)
Kanazawa University Cancer Research Institute(1999/10/01-2003/04/30)
Kanazawa University Cancer Research Institute(2003/05/01-)
Kanazawa University Institute of Liberal Arts and Science(2016/04/01-)

Year & Month of Birth

Academic Society

Japanese Cancer Association
Japanese Association for Metastasis Research Society
Japanese association for oral biology
Japanese Biochemical Society
Japanese Society for Connective Tissue Research

Award

Japanese Society for Connective Tissue Research
Japanese Society for Connective Tissue Research
Japanese Society for Connective Tissue Research

Specialities

Pathological medical chemistry、Experimental pathology

Speciality Keywords

cancer cell, invasion, metastasis, extracellular matrix, matrix metalloproteinase, signal transduction

Research Themes

Molecular Mechanism of Cancer Cell Invasion

Accumulation of mutation in oncogenes and tumor suppressor genes in normal cells results in malignant tumors. Malignant tumors invade into tissues and finally metastasize to distant organs. The goal of our project is to elucidate the molecular mechanism of tumor metastasis and develop diagnostic and therapeutic application.
 Tumor invasion into tissue requires degradation of tissue basement membrane. We discovered a protease which is the key enzyme for tumor metastasis, and named it as MT1-MMP (Nature, 1994). Accumulating evidences indicate that MT1-MMP plays important roles in not only tumor invasion but also regulation of signal transductions which induce tumor growth and migration.

Books

Papers

  •  Simple and cost-effective assay for isolating invasive living cells. Takino T, Sato H, Seiki M. Bio Techniques 65 3 137-142 2018/09/18
  •   Design and synthesis of novel anti-metastatic hypoxic cytotoxin TX-2137 targeting Akt kinase. Shiba I, Kouzaki R, Yamada H, Endo Y, Takino T, Sato H, Kitazato K, Kageji T, Nagahiro S, Uto Y. Anticancer Research 37 7 3877-2884 2017/07
  •  Activation of MMP-9 by membrane type-1 MMP/MMP-2 axis stimulates tumor metastasis Li Z, Takino T, Endo Y, Sato H Cancer Science 108 3 347-353 2017/04/04
  •  Glycogen synthesis 3β sustains invasion of glioblastoma via the focal adhesion kinase, Rac1 and c-Jun N-terminal kinase-mediated pathway.  Chikano Y, Domoto T, Furuta T, Sabit H, Kitano-Tamura A, Pyko IV, Takino T, Sai Y, Hayashi Y, Sato H, Miyamoto KI, Nakada M, Minamoto T Molecular Cancer Therapeutics 14 564 2015
  •  Membrane-type 1 matrix metalloproteinase regulates fibronectin assembly and N-cadherin adhesion.  Takino T, Yoshimoto T, Nakada M, Li Z, Domoto T, Kawashiri S, Sato H Biochemical and Biophysical Research Communications 450 1016 2014

show all

  •  Vinculin negatively regulates transcription of MT1-MMP through MEK/ERK pathway Yoshimoto, Taisuke; Takino, Takahisa; Li, Zichen; Domoto, Takahiro; Sato, Hiroshi Biochemical and Biophysical Research Communications 455 42067 251-255 2014/12/12 
  •  Ligand-dependent EphB1 signaling suppresses glioma invasion and correlates with patient survival Teng, Lei; Nakada, Mitsutoshi; Furuyama, Natsuki; Sabit, Hemragul; Furuta, Takuya; Hayashi, Yutaka; Takino, Takahisa; Dong, Yu; Sato, Hiroshi; Sai, Yoshimichi; Miyamoto, Kenichi; Berens, Michael E.; Zhao, Shiguang; Hamada, Junichiro Neuro-Oncology 15 12 1710-1720 2013/12/01 
  •  MT1-MMP prevents growth inhibition by three dimensional fibronectin matrix.  Takino T, Guo L, Domoto T, Sato H.  BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 436 503 2013/07
  •  Aberrant glycogen synthase kinase 3β is involved in pancreatic cancer cell invasion and resistance to therapy.  Kitano A, Shimasaki T, Chikano Y, Nakada M, Hirose M, Higashi T, Ishigaki Y, Endo Y, Takino T, Sato H, Sai Y, Miyamoto K, Motoo Y, Kawakami K, Minamoto T PLoS One e55289 2013
  •  Integrin α3 is overexpressed in glioma stem-like cells and promotes invasion.  Nakada M, Nambu E, Furuyama N, Yoshida Y, Takino T, Hayashi Y, Sato H, Sai Y, Tsuji T, Miyamoto KI, Hirao A, Hamada JI. British Journal of Cancer 108 2516 2013
  •  Cleavage of hepatocyte growth factor activator inhibitor-1 by membrane-type MMP-1 activates matriptase.  Domoto T, Takino T, Guo LY, Sato H Cancer Science 103 448 2012
  •  Shedding of kidney injury molecule-1 by membrane-type 1 matrix metalloproteinase. Guo L, Takino T, Endo Y, Domoto T, Sato H Journal of Biochemistry 152 425 2012
  •  Membrane-type 1 matrix metalloproteinase regulates fibronectin assembly to promote cell motility Takahisa Takino FEBS LETTERS 585 3378-3384 2011/10
  •  GI24 enhances tumor invasiveness by regulating cell surface membrane-type 1 matrix metalloproteinase.  Sakr M, Takino T, Domoto T, Nakano H, Wong R, Sasaki M, Nakanuma Y, Sato H Cancer Science 101 2368 2010
  •  MT1-MMP promotes cell growth and ERK activation through c-Src and paxillin in three-dimentional collagen matrix BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 396 1042-1047 2010/05
  •  Activation of matrix metalloproteinase-2 (MMP-2) by membrane type 1 matrix metalloproteinase through an artificial receptor for proMMP-2 generates active MMP-2.  Nishida Y, Miyamori H, Thompson EW, Takino T, Endo Y, Sato H Cancer Research 68 9096 2008
  •  Inhibition of membrane-type 1 matrix metalloproteinase at cell-matrix adhesions. T. Takino et al CANCER RESEARCH 67 24 11621-11629 2007/12
  •  Regulation of N-cadherin- based cell-cell interaction by JSAP1 scaffold in PC12h cells. M. Bayarsaikhan, T. Takino et al BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 353 2 357-362 2007/02
  •  Substrate choice of membrane-type 1 matrix metalloproteinase is dictated by tissue inhibitor of metalloproteinase-2 level. T. Kudo, T. Takino et al JAPANESE JOURNAL OF CANCER RESEARCH 98 4 563-568 2007/04
  •  Membrane-type 1 matrix metalloproteinase modulates focal adhesion stability and cell migration. T. Takino et al EXPERIMENTAL CELL RESEARCH 312 8 1381-1389 2006/03
  •  JSAP1/JIP3 cooperates with FAK to regulate c-Jun N-terminal kinase and cell migration. T. Takino et al JOURNAL OF BIOLOGICAL CHEMISTRY 280 45 37772-37781 2005/11
  •  Membrane-type1 matrix metalloproteinase regulates collagen-dependent mitogen- activated protein/extracellular signal-regulated kinase activation and cell migration T. Takino et al. CANCER RESEARCH 64 1044-1049 2004/02
  •  Cleavage of lumican by membrane-type matrix metalloproteinase-1 abrogates this proteoglycan-mediated suppression of tumor cell colony formation in soft agar. Y. Li et al CANCER RESEARCH 64 19 7058-7064 2004/10
  •  CrkI adaptor protein modulates cell migration and invasion in glioblastoma T. Takino et al. CANCER RESEARCH 63 9 2335-2337 2003/06
  •  Tyrosine phosphorylation of the CrkII adaptor protein modulates cell migration T. Takino et al. JOURNAL OF CELL SCIENCE 116 15 3145-3155 2003/08
  •  Cleavage of syndecan-1 by membrane-type matrix metalloproteinase-1 stimulates cell migration. K. Endo, T. Takino et al JOURNAL OF BIOLOGICAL CHEMISTRY 278 42 40764-40770 2003/10
  •  Tetraspanin CD63 promotes targeting and lysosomal proteolysis of membrane-type 1 matrix metalloproteinase. T. Takino et al. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 304 1 160-166 2003/04
  •  Cleavage of metastasis suppressor gene product KiSS-1 protein/Metastin by matrix metalloproteinases. T. Takino et al ONCOGENE 22 30 4617-4626 2003/07
  •  A scaffold protein in the c-Jun N-terminal kinase signaling pathway is associated with focal adhesion kinase and tyrosine-phosphorylated T. Takino et al ONCOGENE 21 42 6488-6497 2002/10
  •  Expression of dominant negative form of Ets-1 suppresses fibronectin-stimulated cell adhesion and migration through down-regulation of integrin alpha 5 expression in U251 glioma cell line. CANCER RESEARCH 61 21 7985-7991 2001/11
  •  Suppression of membrane-type 1 matrix metalloproteinase (MMP)-mediated MMP-2 activation and tumor invasion by testican 3 and its splicing variant gene product, N-Tes. CANCER RESEARCH 61 24 8896-8902 2001/12
  •  Claudin promotes activation of pro-matrix metalloproteinase -2 mediated by membrane-type matrix metalloproteinases. JOURNAL OF BIOLOGICAL CHEMISTRY 276 30 28204-28211 2001/07
  •  Shc and FAK differentially regulate cell motility and directionality modulated by PTEN. JOURNAL OF CELL BIOLOGY 146 2 389-403 1999/07
  •  PTEN interactions with focal adhesion kinase and suppression of the extracellular matrix-dependent phosphatidylinositol 3-kinase/Akt cell survival pathway. JOURNAL OF BIOLOGICAL CHEMISTRY 274 29 20693-206703 1999/07
  •  Tumor suppressor PTEN inhibition of cell invasion, migration, and growth: differential involvement of focal adhesion kinase and p130cas. CANCER RESEARCH 59 2 442-449 1999/01

Conference Presentations

Arts and Fieldwork

Patent

Theme to the desired joint research

Grant-in-Aid for Scientific Research

○「膜型メタロプロテイナーゼ(MFMMP)と乳癌の転移」(1995-1997) 
○「形態形成過程におけるMT1-MMPの機能発現制御機構の解析」(1999-2003) 
○「がん浸潤における細胞運動とMT-MMP発現の協調的制御機構の解析」(2000-2000) 
○「癌細胞運動とマトリックスメタロプロテアーゼ活性発現調節機構の解明」(2000-2001) 
○「がん浸潤における細胞運動とMMP発現の協調的制御機構の解析」(2001-2001) 
○「癌浸潤における細胞運動とMT-MMPの協調的制御機構の解析」(2002-2002) 
○「がん細胞運動とマトリックスメタロプロテアーゼ活性発現の調節機構の解析」(2002-2003) 
○「癌転移における膜型マトリックスメタロプロテアーゼの新規機能の解析」(2002-2003) 
○「癌細胞浸潤における細胞運動とMT-MMPの協調的制御機構の解析」(2003-2003) 
○「がん浸潤の細胞運動と細胞外マトリックス分解御機構の解析」(2004-2004) 
○「JNK結合分子による細胞運動極性制御機構の解析」(2004-2005) 
○「組織破壊に関わるマトリックスメタロプロテアーゼの機能解析」(2005-2006) 
○「JNK結合分子による細胞接着斑と運動極性形成制御機構の解析」(2006-2007) 
○「膜型マトリックスメタロプロテアーゼによる組織破壊機構の解析」(2007-2008) 
○「細胞外微小環境変化と細胞運動誘導」(2008-2010) 
○「膜型マトリックスメタロプロテアーゼによる細胞運動極性制御機構」(2008-2009) 
○「膜型マトリックスメタロプロテアーゼ-1のがん悪性化形質に果たす役割の解析」(2009-2011) 
○「細胞外マトリックス分解と細胞運動の極性形成維持機構」(2011-2013) 
○「肝細胞がんの多様性・変幻性におけるMT1-MMP依存性分子経路の解析」(2016-2018) 

Classes (Bachelors)

○Molecular and Cellular Biology(2017)
○Molecular and Cellular Biology(2017)
○Molecular and Cellular Biology(2017)
○Molecular and Cellular Biology(2017)
○Molecular and Cellular Biology(2017)
○Molecular and Cellular Biology(2017)
○Molecular and Cellular Biology(2017)
○Molecular and Cellular Biology(2017)
○Molecular and Cellular Biology(2017)
○Molecular and Cellular Biology(2017)
○Molecular and Cellular Biology(2017)
○Molecular and Cellular Biology(2017)
○Molecular and Cellular Biology(2017)
○Molecular and Cellular Biology(2017)
○Molecular and Cellular Biology(2017)
○Molecular and Cellular Biology(2017)
○Molecular and Cellular Biology(2017)
○Molecular and Cellular Biology(2017)
○Molecular and Cellular Biology(2017)
○Molecular and Cellular Biology(2017)
○Molecular and Cellular Biology(2016)
○Molecular and Cellular Biology(2016)
○Molecular and Cellular Biology(2016)
○Molecular and Cellular Biology(2016)
○Molecular and Cellular Biology(2016)
○Molecular and Cellular Biology(2016)
○Molecular and Cellular Biology(2016)
○Molecular and Cellular Biology(2016)
○Molecular and Cellular Biology(2016)
○Molecular and Cellular Biology(2016)
○Molecular and Cellular Biology(2016)
○Molecular and Cellular Biology(2016)
○Molecular and Cellular Biology(2016)
○Molecular and Cellular Biology(2016)
○Molecular and Cellular Biology(2016)
○Molecular and Cellular Biology(2016)
○Molecular and Cellular Biology(2016)
○Molecular and Cellular Biology(2016)
○Molecular and Cellular Biology(2016)
○Molecular and Cellular Biology(2016)

Classes (Graduate Schools)

○An Introduction to Cellular Function(2017)
○An Introduction to Cellular Function(2017)
○An Introduction to Cellular Function(2016)
○An Introduction to Cellular Function(2016)

International Project

International Students

Lecture themes

Others (Social Activities)

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