last modified：2024/11/24
Associate Professor SHIRASAKA Yoshiyuki
Faculty, Affiliation
Faculty of Pharmacy, Institute of Medical, Pharmaceutical and Health Sciences
College and School Educational Field
Laboratory
Membrane Transpot and Pharmcokinetics TEL:076-234-4422 FAX:076-264-6284
Academic Background
【Academic background(Doctoral/Master's Degree)】
Setsunan University Doctor Graduate School, Division of Pharmaceutical Sciences 200703 Completed
【Academic background(Bachelor's Degree)】
Setsunan University 200203
【Degree】
Ph.D.
Career
Kanazawa University Faculty of Pharmacy Associate Professor(2020/04-)
Tokyo University of Pharmacy and Life Sciences School of Pharmacy(2017/04-2020/03)
Tokyo University of Pharmacy and Life Sciences School of Pharmacy(2015/01-2017/03)
The Uehara Memorial Foundation Research fellow(2014/01-2014/12)
University of Washington School of Pharmacy Visiting Scholar/Scientist(2012/01-2014/12)
JSPS Postdoctoral Fellow for Research Abroad(2012/01-2014/01)
Kanazawa University Faculty of Pharmacy Assistant Professor(2008/04-2012/01)
Tokyo University of Science Faculty of Pharmaceutical Sciences Assistant Professor(2007/04-2008/03)
Year & Month of Birth
1978/12
Academic Society
The American Society for Pharmacology and Experimental Therapeutics
The Japanese Gastroenterological Association
Japanese Society of Gout and Uric & Nucleic Acids
Japanese Society of Pharmaceutical Health Care and Sciences
The Japanese Society of Digestion and Absorption
The Academy of Pharmaceutical Science and Technology, Japan
The Japanese Society for the Study of Xenobiotics
The Japanese Society of Toxicology
The Pharmaceutical Society of Japan
The Japanese Society of Clinical Pharmacology and Therapeutics
Forum for Pharmaceutical Technology Innovation
Japan Transporter Research Association
The International Society for the Study of Xenobiotics
The American Association of Pharmaceutical Scientists
Award
○APSTJ Focus Group Presentation Award(2008)
○10th International ISSX Poster Award, Finalist(2013)
○JSSX Best Presentation Award 2009, 1st Place(2009)
○JSSX Best Presentation Award 2009, Finalist(2010)
○DMPK Award for the Most Frequently Downloaded Article in 2006, 3rd Place(2008)
○JTRA Kanto Best Presentation Award 2018(2018)
○PSJ Hokuriku Best Presentation Award 2010(2010)
○APSTJ Global Education Seminar Presentation Award 2009(2009)
○PSJ Kanto Best Presentation Award 2018(2018)
○24th APSTJ Best Presentation Award(2009)
○3rd Asia/Pacific ISSX Best Presentation Award, 2nd Place(2009)
○JSSX Best Presentation Award 2021(2021)
○34th APSTJ Best Presentation Award(2019)
○YCPS 2021 Best Presentation Award(2021)
○PSJKB Award for Young Pharmaceutical Scientist 2018(2018)
○APSTJ Award for Young Pharmaceutical Scientist 2017(2017)
○4th Asia/Pacific ISSX Best Presentation Award, 1st Place(2011)
○JSPHCS 3rd Fresher's Conference Best Presentation Award(2019)
○YCPS 2019 Best Presentation Award(2019)
○Postdoctoral Presentation Award 2009(2009)
○JSSX Award for Young Scientists 2015(2015)
○JTRA Kanto Presentation Award 2019(2019)
○The 3rd Workshop for Japan-Korea Young Scientists on Pharmaceutics, Best Poster Award(2019)
○JTRA Kanto Presentation Award 2008(2018)
○APSTJ Global Education Seminar Presentation Award 2013(2013)
Specialities
Pharmacokinetics
Speciality Keywords
Pharmacokinetics,Biopharmaceutics,Drug Absorption,Drug Interaction,Gastrointestinal Toxicity,Drug Transport,Drug Metabolism,Bioavailability
Research Themes
Kinetic Analysis on Counteracting Interplay of Influx and Efflux transporters on Intestinal Drud Absorption
Books
Papers
- Effect of ingested fluid volume and solution osmolality on intestinal drug absorption: Impact on drug interaction with beverages Yuta Funai,Miyuki Takemura,Katsuhisa Inoue,Yoshiyuki Shirasaka European Journal of Pharmaceutical Sciences 172 106136 2022/05
- Characterization of Aripiprazole Uptake Transporter in the Blood-Brain Barrier Model hCMEC/D3 Cells by Targeted siRNA Screening Moeno Kadoguchi,Hiroshi Arakawa,Ryokichi Honda,Kazuki Hotta,Yoshiyuki Shirasaka,Yoshiharu Deguchi,Ikumi Tamai Pharmaceutical Research 2022/03
- Evaluation of Platinum Anticancer Drug-Induced Kidney Injury in Primary Culture of Rat Kidney Tissue Slices by Using Gas-Permeable Plates Hiroshi Arakawa,Yurika Nagao,Shiho Nedachi,Yoshiyuki Shirasaka,Ikumi Tamai Biological and Pharmaceutical Bulletin 45 3 316 2022/03
- Multiple Transport Mechanisms Involved in the Intestinal Absorption of Metformin: Impact on the Nonlinear Absorption Kinetics Yoshiyuki Shirasaka,Maria Seki,Marie Hatakeyama,Yuko Kurokawa,Hiroki Uchiyama,Miyuki Takemura,Yugo Yasugi,Hisanao Kishimoto,Ikumi Tamai,Joanne Wang,Katsuhisa Inoue Journal of Pharmaceutical Sciences 111 5 1531 2022/01
- Influence of osmolality on gastrointestinal fluid volume and drug absorption: potential impact on oral salt supplementation Miyuki Takemura,Yuki Tanaka,Katsuhisa Inoue,Ikumi Tamai,Yoshiyuki Shirasaka Journal of Pharmaceutical Health Care and Sciences 7 1 29 2021/12
- Model Analysis of the Apparent Saturation Kinetics of Purine Nucleobase Uptake in Cells co-Expressing Transporter and Metabolic Enzyme Satoru Suzuki,Katsuhisa Inoue,Ikumi Tamai,Yoshiyuki Shirasaka Pharmaceutical Research 38 9 1585 2021/09
- Utilization of Sodium Nitroprusside as an Intestinal Permeation Enhancer for Lipophilic Drug Absorption Improvement in the Rat Proximal Intestine Hisanao Kishimoto,Kaori Miyazaki,Hiroshi Tedzuka,Ryosuke Ozawa,Hanai Kobayashi,Yoshiyuki Shirasaka,Katsuhisa Inoue Molecules 26 21 6396 2021/10/22
- 3CL Protease Inhibitors with an Electrophilic Arylketone Moiety as Anti-SARS-CoV-2 Agents. Sho Konno,Kiyotaka Kobayashi,Miki Senda,Yuta Funai,Yuta Seki,Ikumi Tamai,Laura Schäkel,Kyousuke Sakata,Thanigaimalai Pillaiyar,Akihiro Taguchi,Atsuhiko Taniguchi,Michael Gütschow,Christa E Müller,Koh Takeuchi,Mikako Hirohama,Atsushi Kawaguchi,Masaki Kojima,Toshiya Senda,Yoshiyuki Shirasaka,Wataru Kamitani,Yoshio Hayashi Journal of medicinal chemistry 65 4 2926 2021/07/27
- Quantitative analysis of gastrointestinal physiology for better prediction of oral drug absorption and interaction. Shirasaka Y Yakugaku-Zasshi. 140 5 599 2020
- Functional characterization of monocarboxylate transporter 12 (SLC16A12/MCT12) as a facilitative creatine transporter. Takahashi M,Kishimoto H,Shirasaka Y,Inoue K Drug Metab Pharmacokinet. 35 3 281 2020
- Quantitative analysis of the effect of controlled-release formulation on nonlinear gastrointestinal absorption of P-glycoprotein substrate talinolol using physiologically based pharmacokinetic absorption model. Suzuki S,Shirasaka Y,Okada R,Eguchi A,Kishimoto H,Langguth P,Inoue K Journal of Drug Delivery Science and Technology 56 Part B 2020
- Mucins are Involved in the Intestinal Permeation of Lipophilic Drugs in the Proximal Region of Rat Small Intestine. Miyazaki K,Kishimoto H,Muratani M,Kobayashi H,Shirasaka Y,Inoue K Pharm Res. 36 11 162 2019
- Effect of osmolality on the pharmacokinetic interaction between apple juice and atenolol in rats. Funai Y,Shirasaka Y,Ishihara M,Takemura M,Ichijo K,Kishimoto H,Inoue K Drug Metab Dispos. 47 4 386 2019
- Organic anion transporter 1 (OAT1/SLC22A6) enhances bioluminescence based on D-luciferin–luciferase reaction in living cells by facilitating the intracellular accumulation of D-luciferin Takahito Furuya,Issey Takehara,Asuka Shimura,Hisanao Kishimoto,Tomoya Yasujima,Kinya Ohta,Yoshiyuki Shirasaka,Hiroaki Yuasa,Katsuhisa Inoue Biochemical and Biophysical Research Communications 495 3 2152 2018/01/15
- Interspecies comparison of the functional characteristics of plasma membrane monoamine transporter (PMAT) between human, rat and mouse Yoshiyuki Shirasaka,Nora Lee,Haichuan Duan,Horace Ho,Joanna Pak,Joanne Wang Journal of Chemical Neuroanatomy 83-84 99 2017/10
- Osmolality of Orally Administered Solutions Influences Luminal Water Volume and Drug Absorption in Intestine Kazuki Ichijo,Rika Oda,Marika Ishihara,Ren Okada,Yuri Moteki,Yuta Funai,Takuya Horiuchi,Hisanao Kishimoto,Yoshiyuki Shirasaka,Katsuhisa Inoue Journal of Pharmaceutical Sciences 106 9 2889 2017/09
- Physiologically based pharmacokinetic model of all- trans-retinoic acid with application to cancer populations and drug interactions. Jing J,Nelson C,Paik J,Shirasaka Y,Amory JK,Isoherranen N Journal of Pharmacology and Experimental Therapeutics 361 5 385 2017/02
- Involvement of organic cation transporter 3 (Oct3/Slc22a3) in the bioavailability and pharmacokinetics of antidiabetic metformin in mice Yoshiyuki Shirasaka,Nora Lee,Weibin Zha,David Wagner,Joanne Wang Drug Metabolism and Pharmacokinetics 31 5 385 2016/10
- Interindividual variability of CYP2C19-catalyzed drug metabolism due to differences in gene diplotypes and cytochrome P450 oxidoreductase content Y Shirasaka,A S Chaudhry,M McDonald,B Prasad,T Wong,J C Calamia,A Fohner,T A Thornton,N Isoherranen,J D Unadkat,A E Rettie,E G Schuetz,K E Thummel The Pharmacogenomics Journal 16 4 375 2016/08
- Absorption-Enhancing Effect of Nitric Oxide on the Absorption of Hydrophobic Drugs in Rat Duodenum Hisanao Kishimoto,Kaori Miyazaki,Yusuke Takizawa,Yoshiyuki Shirasaka,Katsuhisa Inoue Journal of Pharmaceutical Sciences 105 2 729 2016/02
- Atenolol Renal Secretion Is Mediated by Human Organic Cation Transporter 2 and Multidrug and Toxin Extrusion Proteins Jia Yin,Haichuan Duan,Yoshiyuki Shirasaka,Bhagwat Prasad,Joanne Wang Drug Metabolism and Disposition 43 12 1872 2015/12
- TheCYP2C19Intron 2 Branch Point SNP is the Ancestral Polymorphism Contributing to the Poor Metabolizer Phenotype in Livers withCYP2C19*35andCYP2C19*2Alleles Amarjit S. Chaudhry,Bhagwat Prasad,Yoshiyuki Shirasaka,Alison Fohner,David Finkelstein,Yiping Fan,Shuoguo Wang,Gang Wu,Eleni Aklillu,Sarah C. Sim,Kenneth E. Thummel,Erin G. Schuetz Drug Metabolism and Disposition 43 8 1226 2015/08
- Substrate- and Dose-Dependent Drug Interactions with Grapefruit Juice Caused by Multiple Binding Sites on OATP2B1 Yoshiyuki Shirasaka,Takanori Mori,Yukiko Murata,Takeo Nakanishi,Ikumi Tamai Pharmaceutical Research 31 8 2035 2014/08
- Effect of CYP3A5 Expression on the Inhibition of CYP3A-Catalyzed Drug Metabolism: Impact on Modeling CYP3A-Mediated Drug-Drug Interactions Yoshiyuki Shirasaka,Shu-Ying Chang,Mary F. Grubb,Chi-Chi Peng,Kenneth E. Thummel,Nina Isoherranen,A. David Rodrigues Drug Metabolism and Disposition 41 8 1566 2013/08
- Inhibition of CYP2C19 and CYP3A4 by Omeprazole Metabolites and Their Contribution to Drug-Drug Interactions Yoshiyuki Shirasaka,Jennifer E. Sager,Justin D. Lutz,Connie Davis,Nina Isoherranen Drug Metabolism and Disposition 41 7 1414 2013/07
- A role of prostaglandin transporter in regulating PGE(2) release from human bronchial epithelial BEAS-2B cells in response to LPS Yoshiyuki Shirasaka,Megumi Shichiri,Taku Kasai,Yasuhiro Ohno,Takeo Nakanishi,Kazuyuki Hayashi,Akio Nishiura,Ikumi Tamai JOURNAL OF ENDOCRINOLOGY 217 3 265 2013/06
- Long-lasting inhibitory effect of apple and orange juices, but not grapefruit juice, on OATP2B1-mediated drug absorption Yoshiyuki Shirasaka,Megumi Shichiri,Yukiko Murata,Takanori Mori,Takeo Nakanishi,Ikumi Tamai Drug Metabolism and Disposition 41 3 615 2013/03
- Major Active Components in Grapefruit, Orange, and Apple Juices Responsible for OATP2B1-Mediated Drug Interactions Yoshiyuki Shirasaka,Megumi Shichiri,Takanori Mori,Takeo Nakanishi,Ikumi Tamai Journal of Pharmaceutical Sciences 102 1 280 2013/01
- Major active components in grapefruit, orange, and apple juices responsible for OATP2B1-mediated drug interactions Yoshiyuki Shirasaka,Megumi Shichiri,Takanori Mori,Takeo Nakanishi,Ikumi Tamai Journal of Pharmaceutical Sciences 102 9 3418 2013/09
- MDR1 is related to intestinal epithelial injury induced by acetylsalicylic acid. Munehiro Kugai,Kazuhiko Uchiyama,Toshifumi Tsuji,Hiroyuki Yoriki,Akifumi Fukui,Ying Qin,Yasuki Higashimura,Katsura Mizushima,Naohisa Yoshida,Kazuhiro Katada,Kazuhiro Kamada,Osamu Handa,Tomohisa Takagi,Hideyuki Konishi,Nobuaki Yagi,Toshikazu Yoshikawa,Yoshiyuki Shirasaka,Ikumi Tamai,Yuji Naito,Yoshito Itoh Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 32 4 942 2013
- Active intestinal absorption of fluoroquinolone antibacterial agent ciprofloxacin by organic anion transporting polypeptide, Oatp1a5 Hiroshi Arakawa,Yoshiyuki Shirasaka,Makoto Haga,Takeo Nakanishi,Ikumi Tamai BIOPHARMACEUTICS & DRUG DISPOSITION 33 6 332 2012/09
- Functional Pleiotropy of Organic Anion Transporting Polypeptide OATP2B1 Due to Multiple Binding Sites Yoshiyuki Shirasaka,Takanori Mori,Megumi Shichiri,Takeo Nakanishi,Ikumi Tamai Drug Metabolism and Pharmacokinetics 27 3 360 2012/06
- Involvement of Functional Groups on the Surface of Carboxyl Group-Terminated Polyamidoamine Dendrimers Bearing Arbutin in Inhibition of Na+/Glucose Cotransporter 1 (SGLT1)-Mediated D-Glucose Uptake Shinji Sakuma,Shun Kanamitsu,Yumi Teraoka,Yoshie Masaoka,Makoto Kataoka,Shinji Yamashita,Yoshiyuki Shirasaka,Ikumi Tamai,Masahiro Muraoka,Yohji Nakatsuji,Toshiyuki Kida,Mitsuru Akashi MOLECULAR PHARMACEUTICS 9 4 922 2012/04
- Functional Characterization of Apical Transporters Expressed in Rat Proximal Tubular Cells (PTCs) in Primary Culture Takeo Nakanishi,Akimasa Fukushi,Masanobu Sato,Mayuko Yoshifuji,Tomoka Gose,Yoshiyuki Shirasaka,Kazuyo Ohe,Masato Kobayashi,Keiichi Kawai,Ikumi Tamai MOLECULAR PHARMACEUTICS 8 6 2142 2011/11
- Estimation of Michaelis-Menten constant of efflux transporter considering asymmetric permeability Kiyohiko Sugano,Yoshiyuki Shirasaka,Shinji Yamashita INTERNATIONAL JOURNAL OF PHARMACEUTICS 418 2 161 2011/10
- Differential Effect of Grapefruit Juice on Intestinal Absorption of Statins Due to Inhibition of Organic Anion Transporting Polypeptide and/or P-glycoprotein Yoshiyuki Shirasaka,Kensuke Suzuki,Takeo Nakanishi,Ikumi Tamai Journal of Pharmaceutical Sciences 100 9 3843 2011/09
- Identification and functional characterization of uric acid transporter Urat1 (Slc22a12) in rats Masanobu Sato,Tomohiko Wakayama,Hideaki Mamada,Yoshiyuki Shirasaka,Takeo Nakanishi,Ikumi Tamai Biochimica et Biophysica Acta (BBA) - Biomembranes 1808 6 1441 2011/06
- Quantitative Time-Lapse Imaging-Based Analysis of Drug-Drug Interaction Mediated by Hepatobiliary Transporter, Multidrug Resistance-Associated Protein 2, in Sandwich-Cultured Rat Hepatocytes Takeo Nakanishi,Yuta Shibue,Yoko Fukuyama,Kenji Yoshida,Hajime Fukuda,Yoshiyuki Shirasaka,Ikumi Tamai DRUG METABOLISM AND DISPOSITION 39 6 984 2011/06
- The effects of the SLCO2B1 c.1457C > T polymorphism and apple juice on the pharmacokinetics of fexofenadine and midazolam in humans Junko Imanaga,Tsutomu Kotegawa,Hiromitsu Imai,Kimiko Tsutsumi,Tsuneaki Yoshizato,Tetsuji Ohyama,Yoshiyuki Shirasaka,Ikumi Tamai,Tomonori Tateishi,Kyoichi Ohashi PHARMACOGENETICS AND GENOMICS 21 2 84 2011/02
- Organic Cation Transporter-Mediated Renal Secretion of Ipratropium and Tiotropium in Rats and Humans Takeo Nakanishi,Tsunemitsu Haruta,Yoshiyuki Shirasaka,Ikumi Tamai DRUG METABOLISM AND DISPOSITION 39 1 117 2011/01
- Intestinal Absorption of HMG-CoA Reductase Inhibitor Pitavastatin Mediated by Organic Anion Transporting Polypeptide and P-Glycoprotein/Multidrug Resistance 1 Yoshiyuki Shirasaka,Kensuke Suzuki,Megumi Shichiri,Takeo Nakanishi,Ikumi Tamai DRUG METABOLISM AND PHARMACOKINETICS 26 2 171 2011
- Carnitine Precursor gamma-Butyrobetaine is a Novel Substrate of the Na+- and Cl--dependent GABA Transporter Gat2 Takeo Nakanishi,Yoko Fukuyama,Masaharu Fujita,Yoshiyuki Shirasaka,Ikumi Tamai DRUG METABOLISM AND PHARMACOKINETICS 26 6 632 2011
- Intestinal Absorption of HMG-CoA Reductase Inhibitor Pravastatin Mediated by Organic Anion Transporting Polypeptide Yoshiyuki Shirasaka,Kensuke Suzuki,Takeo Nakanishi,Ikumi Tamai Pharmaceutical Research 27 10 2141 2010/10
- Intestinal Absorption Mechanism of Tebipenem Pivoxil, a Novel Oral Carbapenem: Involvement of Human OATP Family in Apical Membrane Transport Kazuhiko Kato,Yoshiyuki Shirasaka,Erika Kuraoka,Akihiro Kikuchi,Maki Iguchi,Hisashi Suzuki,Shigeki Shibasaki,Tohru Kurosawa,Ikumi Tamai MOLECULAR PHARMACEUTICS 7 5 1747 2010/09
- Carboxyl group-terminated polyamidoamine dendrimers bearing glucosides inhibit intestinal hexose transporter-mediated D-glucose uptake Shinji Sakuma,Yumi Teraoka,Tomokazu Sagawa,Yoshie Masaoka,Makoto Kataoka,Shinji Yamashita,Yoshiyuki Shirasaka,Ikumi Tamai,Yusuke Ikumi,Toshiyuki Kida,Mitsuru Akashi EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS 75 3 366 2010/08
- Quantitation of Talinolol in Rat Plasma By LC-MS-MS Yan Li,Yoshiyuki Shirasaka,Peter Langguth,Ikumi Tamai JOURNAL OF CHROMATOGRAPHIC SCIENCE 48 5 367 2010/05
- Involvement of Choline Transporter-Like Proteins, CTL1 and CTL2, in Glucocorticoid-Induced Acceleration of Phosphatidylcholine Synthesis via Increased Choline Uptake Toshimichi Nakamura,Ryohei Fujiwara,Naoki Ishiguro,Masanobu Oyabu,Takeo Nakanishi,Yoshiyuki Shirasaka,Tomoji Maeda,Ikumi Tamai BIOLOGICAL & PHARMACEUTICAL BULLETIN 33 4 691 2010/04
- Model Analysis of the Concentration-Dependent Permeability of P-gp Substrates Tatsuhiko Tachibana,Satoshi Kitamura,Motohiro Kato,Tetsuya Mitsui,Yoshiyuki Shirasaka,Shinji Yamashita,Yuichi Sugiyama PHARMACEUTICAL RESEARCH 27 3 442 2010/03
- Renal Secretion of Uric Acid by Organic Anion Transporter 2 (OAT2/SLC22A7) in Human Masanobu Sato,Hideaki Mamada,Naohiko Anzai,Yoshiyuki Shirasaka,Takeo Nakanishi,Ikumi Tamai BIOLOGICAL & PHARMACEUTICAL BULLETIN 33 3 498 2010/03
- Species Difference in the Effect of Grapefruit Juice on Intestinal Absorption of Talinolol between Human and Rat Yoshiyuki Shirasaka,Erika Kuraoka,Hildegard Spahn-Langguth,Takeo Nakanishi,Peter Langguth,Ikumi Tamai Journal of Pharmacology and Experimental Therapeutics 332 1 181 2010/01
- Transport of Ipratropium, an Anti-Chronic Obstructive Pulmonary Disease Drug, Is Mediated by Organic Cation/Carnitine Transporters in Human Bronchial Epithelial Cells: Implications for Carrier-Mediated Pulmonary Absorption Toshimichi Nakamura,Takeo Nakanishi,Tsunemitsu Haruta,Yoshiyuki Shirasaka,John P. Keogh,Ikumi Tamai MOLECULAR PHARMACEUTICS 7 1 187 2010/01
- Hepatic uptake of gamma-butyrobetaine, a precursor of carnitine biosynthesis, in rats Masaharu Fujita,Takeo Nakanishi,Yuta Shibue,Daisuke Kobayashi,Richard H. Moseley,Yoshiyuki Shirasaka,Ikumi Tamai AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY 297 4 G681 2009/10
- Oseltamivir (Tamiflu) Is a Substrate of Peptide Transporter 1 Takuo Ogihara,Takashi Kano,Tamae Wagatsuma,Sho Wada,Hikaru Yabuuchi,Shigeki Enomoto,Kaori Morimoto,Yoshiyuki Shirasaka,Shoko Kobayashi,Ikumi Tamai DRUG METABOLISM AND DISPOSITION 37 8 1676 2009/08
- Organic anion transporter OAT1 is involved in renal handling of citrulline Masanori Nakakariya,Yoichiro Shima,Yoshiyuki Shirasaka,Keisuke Mitsuoka,Takeo Nakanishi,Ikumi Tamai AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY 297 1 F71 2009/07
- Expression levels of human P-glycoprotein inIn Vitrocell lines: correlation between mRNA and protein levels for P-glycoprotein expressed in cells Yoshiyuki Shirasaka,Reiko Konishi,Nana Funami,Yuko Kadowaki,Yuri Nagai,Toshiyuki Sakaeda,Shinji Yamashita Biopharmaceutics & Drug Disposition 30 3 149 2009/04
- Transport Characteristics of L-Citrulline in Renal Apical Membrane of Proximal Tubular Cells Keisuke Mitsuoka,Yoshiyuki Shirasaka,Akimasa Fukushi,Masanobu Sato,Toshimichi Nakamura,Takeo Nakanishi,Ikumi Tamai BIOPHARMACEUTICS & DRUG DISPOSITION 30 3 126 2009/04
- Concentration-Dependent Effect of Naringin on Intestinal Absorption of beta(1)-Adrenoceptor Antagonist Talinolol Mediated by P-Glycoprotein and Organic Anion Transporting Polypeptide (Oatp) Yoshiyuki Shirasaka,Yan Li,Yuta Shibue,Erika Kuraoka,Hildegard Spahn-Langguth,Yukio Kato,Peter Langguth,Ikumi Tamai PHARMACEUTICAL RESEARCH 26 3 560 2009/03
- Stabilization of enzyme-susceptible glucoside bonds of phloridzin through conjugation with poly(gamma-glutamic acid) Shinji Sakuma,Tomokazu Sagawa,Yoshie Masaoka,Makoto Kataoka,Shinji Yamashita,Yoshiyuki Shirasaka,Ikumi Tamai,Yusuke Ikumi,Toshiyuki Kida,Mitsuru Akashi JOURNAL OF CONTROLLED RELEASE 133 2 125 2009/01
- Scaling of in Vitro Membrane Permeability to Predict P-glycoprotein-Mediated Drug Absorption in Vivo Yoshiyuki Shirasaka,Yoshie Masaoka,Makoto Kataoka,Shinji Sakuma,Shinji Yamashita Drug Metabolism and Disposition 36 5 916 2008/05
- Characterization of gastrointestinal drug absorption in cynomolgus monkeys Masayuki Takahashi,Takuo Washio,Norio Suzuki,Katsuhiro Igeta,Yoshimine Fujii,Masahiro Hayashi,Yoshiyuki Shirasaka,Shinji Yamashita MOLECULAR PHARMACEUTICS 5 2 340 2008/03
- Effect of P‐Glycoprotein Expression Levels on the Concentration‐Dependent Permeability of Drugs to the Cell Membrane Yoshiyuki Shirasaka,Toshiyasu Sakane,Shinji Yamashita Journal of Pharmaceutical Sciences 97 1 553 2008/01
- Oseltamivir (Tamiflu) efflux transport at the blood-brain barrier via P-glycoprotein Kaori Morimoto,Masanori Nakakariya,Yoshiyuki Shirasaka,Chihaya Kakinuma,Takuya Fujita,Ikumi Tamai,Takuo Ogihara DRUG METABOLISM AND DISPOSITION 36 1 6 2008/01
- Effects of Angiotensin II Receptor Blockers on Renal Handling of Uric Acid in Rats Yan Li,Masanobu Sato,Yuichi Yanagisawa,Hideaki Mamada,Akimasa Fukushi,Kentaro Mikami,Yoshiyuki Shirasaka,Ikumi Tamai DRUG METABOLISM AND PHARMACOKINETICS 23 4 263 2008
- Evaluation of Human P-Glycoprotein (MDR1/ABCB1) ATPase Activity Assay Method by Comparing with in Vitro Transport Measurements: Michaelis-Menten Kinetic Analysis to Estimate the Affinity of P-Glycoprotein to Drugs Yoshiyuki Shirasaka,Yuko Onishi,Aki Sakurai,Hiroshi Nakagawa,Toshihisa Ishikawa,Shinji Yamashita Biological and Pharmaceutical Bulletin 29 12 2465 2006/12
- Induction of Human P-Glycoprotein in Caco-2 cells: Development of a Highly Sensitive Assay System for P-Glycoprotein-Mediated Drug Transport Yoshiyuki Shirasaka,Masae Kawasaki,Toshiyasu Sakane,Hideaki Omatsu,Yuka Moriya,Tsutomu Nakamura,Toshiyuki Sakaeda,Katsuhiko Okumura,Peter Langguth,Shinji Yamashita Drug Metabolism and Pharmacokinetics 21 5 414 2006
- Macrolide and Ketolide Antibiotics Inhibit the Cytotoxic Effect of Trastuzumab Emtansine in HER2-Positive Breast Cancer Cells: Implication of a Potential Drug–ADC Interaction in Cancer Chemotherapy Keisuke Kiyomiya,Ryuto Tomabechi,Naoki Saito,Kenta Watai,Tappei Takada,Yoshiyuki Shirasaka,Hisanao Kishimoto,Kei Higuchi,Katsuhisa Inoue Molecular Pharmaceutics 2023/11/16
- SLC46A3 is a lysosomal proton-coupled steroid conjugate and bile acid transporter involved in transport of active catabolites of T-DM1 Ryuto Tomabechi,Hisanao Kishimoto,Taeka Sato,Naoki Saito,Keisuke Kiyomiya,Tappei Takada,Kei Higuchi,Yoshiyuki Shirasaka,Katsuhisa Inoue PNAS Nexus 1 3 2022/07
- Identification of 5-Carboxyfluorescein as a Probe Substrate of SLC46A3 and Its Application in a Fluorescence-Based In Vitro Assay Evaluating the Interaction with SLC46A3 Ryuto Tomabechi,Miki Miyasato,Taeka Sato,Tappei Takada,Kei Higuchi,Hisanao Kishimoto,Yoshiyuki Shirasaka,Katsuhisa Inoue Molecular Pharmaceutics 20 1 491 2023/01
- Apple juice relieves loperamide-induced constipation in rats by downregulating the intestinal apical sodium-dependent bile acid transporter ASBT Qiunan Zhu,Ryusuke Iwai,Takehiro Okaguchi,Yoshiyuki Shirasaka,Ikumi Tamai Food & Function 14 10 4836 2023/05
- Food effects on oral drug absorption: Recent advances in understanding mechanisms and quantitative prediction Yoshiyuki Shirasaka,Atsushi Kambayashi Drug Metabolism and Pharmacokinetics 54 100533 2024/02
- Quantitative analysis of gastrointestinal fluid absorption and secretion to estimate luminal fluid dynamics in rats Yuta Funai,Kazuki Ichijo,Satoru Suzuki,Yuta Tateishi,Katsuhisa Inoue,Ikumi Tamai,Yoshiyuki Shirasaka Scientific Reports 13 1 2023/10/14
- Potentiation of the Uricosuric Effect of Dotinurad byTrans-Inhibition of the Uric Acid Reabsorptive Transporter URAT1 Kazuki Fujita,Qiunan Zhu,Hiroshi Arakawa,Yoshiyuki Shirasaka,Ikumi Tamai Drug Metabolism and Disposition DMD 2023/08/29
- Quantitative Analysis of Gastrointestinal Water Dynamics by Means of a Physiologically Based Fluid Kinetic Model Satoru Suzuki,Katsuhisa Inoue,Ikumi Tamai,Yoshiyuki Shirasaka The AAPS Journal 25 3 2023/04/20
- Food effects on gastrointestinal physiology and drug absorption Atsushi Kambayashi,Yoshiyuki Shirasaka Drug Metabolism and Pharmacokinetics 48 100488 2023/02
- Induction of open-form bile canaliculus formation by hepatocytes for evaluation of biliary drug excretion. Hiroshi Arakawa,Yuya Nakazono,Natsumi Matsuoka,Momoka Hayashi,Yoshiyuki Shirasaka,Atsushi Hirao,Ikumi Tamai Communications biology 6 1 866 2023/08/22
- Apple-derived extracellular vesicles modulate the expression of human intestinal bile acid transporter ASBT/SLC10A2 via downregulation of transcription factor RARα Shinya Usui,Qiunan Zhu,Hisakazu Komori,Yui Iwamoto,Takumi Nishiuchi,Yoshiyuki Shirasaka,Ikumi Tamai Drug Metabolism and Pharmacokinetics 52 100512 2023/10
- Quantitative analysis of the impact of membrane permeability on intestinal first‐pass metabolism of CYP3A substrates Yugo Yasugi,Yoshiyuki Shirasaka,Ikumi Tamai Biopharmaceutics & Drug Disposition 2023/12/12
- The Use of Carboxyfluorescein Reveals the Transport Function of MCT6/SLC16A5 Associated with CD147 as a Chloride-Sensitive Organic Anion Transporter in Mammalian Cells Koki Sugiyama,Hiroe Shimano,Masaki Takahashi,Yuta Shimura,Asuka Shimura,Takahito Furuya,Ryuto Tomabechi,Yoshiyuki Shirasaka,Kei Higuchi,Hisanao Kishimoto,Katsuhisa Inoue Journal of Pharmaceutical Sciences 2023/12
- Magnitude of Fruit Juice–Drug Interactions Due to Osmolality-Dependent Fluid Secretion: Differences among Apple, Orange, and Grapefruit Juices Miyuki Takemura,Katsuhisa Inoue,Ikumi Tamai,Yoshiyuki Shirasaka Biological and Pharmaceutical Bulletin 47 1 72 2024/01/01
- The GlycosylatedN-Terminal Domain of MUC1 Is Involved in Chemoresistance by Modulating Drug Permeation Across the Plasma Membrane Kaori Miyazaki,Hisanao Kishimoto,Hanai Kobayashi,Ayaka Suzuki,Kei Higuchi,Yoshiyuki Shirasaka,Katsuhisa Inoue Molecular Pharmacology 103 3 166 2023/03
Conference Presentations
- Attenuation of Oral Absorption of P-glycoprotein Substrate Drugs by Administrating in Controlled-Release Formulation(conference：International Symposium on BA/BE of Oral Drug Products)(2011/06/30)
- Intestinal Absorption of Drugs Controlled by Influx/Efflux Transporters(conference：131st PSJ Annual Meeting)(2011/03/29)
- Characteristic and Kinetic Analysis of Gastrointestinal Drug Concentration: Impact on Drug Absorption, Transport and Delivery(conference：University of Washington, School of Pharmacy, Special Seminar)(2016/11/18)
- Impact of Water Absorption/Secretion on the Intestinal Drug Absorption and Drug-Beverage Interactions(conference：2016 AAPS Annual Meeting and Exposition)(2016/11/13)
- Characteristic and Kinetic Analysis of Gastrointestinal Water Dynamics to Predict Oral Drug Absorption and Interaction(conference：University of Washington, School of Pharmacy, Special Seminar)(2019/07/18)
- Multiple Transport Mechanisms Involved in the Intestinal Absorption and Disposition of Antidiabetic Metformin(conference：19th North American ISSX Meeting/29th JSSX Meeting)(2014/10/19)
- Role of Drug Absorption and Pharmacokinetics in Drug Discovery: Mechanistic and Kinetic Analysis of Intestinal Drug Absorption(conference：University of Hyogo Leading Program International Symposium 2017)(2017/12/04)
- Characteristic and Kinetic Analysis of Gastrointestinal Water Dynamics: Impact on Oral Drug Absorption(conference：3rd International Symposium on BA/BE of Oral Drug Products (Session 2))(2018/10/11)
- Research Activities at University of Washington –Based on Research Abroad for Improving English, Experiencing Other Cultures, Meeting New People, Challenging Myself, and Becoming Global Expert in Pharmaceutics-(conference：Setsunan University, Special Lecture)(2013/05/29)
- Impact of CYP3A5 Expression on the Inhibition of CYP3A-Catalyzed Drug Metabolism: Considerations for Modeling CYP3A-Mediated Drug-Drug Interactions(conference：18th North American Regional ISSX Meeting)(2012/10/15)
- Now is the time to unify pharmaceutical education into the six-year system: Influence of disarray in an education system between national/public and private universities on fostering of researchers in future generations(conference：33rd APSTJ Annual Meeting)(2018/06/01)
- Characteristic and Kinetic Analysis of Gastrointestinal Water Dynamics to Predict Oral Drug Absorption and Interaction(conference：7th International Postgraduate Conference on Pharmaceutical Sciences (iPoPS2020))(2020/02/29)
- Species Difference in the Transporter-Mediated Drug Absorption between Human and Rat -Impact of OATP and P-gp on Grapefruit Juice-Drug Interaction-(conference：University of Washington, Special Seminar)(2012/09/27)
- Inhibition of CYP2C19 and CYP3A4 by Omeprazole Metabolites and Their Contribution to Drug-Drug Interactions(conference：28th APSTJ Annual Meeting)(2013/05/25)
- The Movement of History -Ten Years since the Beginning of SNPEE-(conference：30th APSTJ Annual Meeting SNPEE2015)(2015/05/22)
- Research Activities at University of Washington -Risk Assessment of Cytochrome P450-Mediated Drug-Drug Interactions-(conference：Kanazawa University, Special Seminar)(2013/05/27)
- New challenges of pharmaceutical-pharmacokinetic collaboration in innovative drug development(2017/05/13)
- Kinetic Analysis of Physiological Functions of Gastrointestinal Tract to Predict Oral Drug Absorption(conference：32nd APSTJ Annual Meeting)(2017/05/13)
- Characteristic and Kinetic Analysis of Gastrointestinal Water Dynamics: Impact on Drug Absorption, Transport and Interaction(2019/02/05)
- Education of Pharmacists and Pharmaceutical Researchers in Japan: Current Problems, Suggestions and Future Challenges(conference：7th Conference of Asia Pacific Pharmacy Education Network (AP-PEN))(2018/10/07)
- Interindividual Variability of CYP2C19-Catalyzed Drug Metabolism Due to Differences in Gene Diplotypes and Cytochrome P450 Oxidoreductase Content(conference：2014 AAPS Annual Meeting and Exposition)(2014/11/02)
- Interindividual Variability of CYP2C19-Catalyzed Drug Metabolism Due to Differences in the Diplotypes(conference：10th International ISSX Meeting)(2013/09/30)
- Research Activities at University of Washington -Predictions of Transporter- and Metabolic Enzymes (P450)-Mediated Drug-Drug Interactions-(conference：Ritsumeikan University, Special Seminar)(2013/05/28)
- Impact of CYP3A5 Expression on CYP3A-Mediated Drug-Drug Interactions –Based on Research Abroad for Improving English, Experiencing Other Cultures, Meeting New People, Challenging Myself, and Becoming Global Expert in Pharmaceutics-(conference：28th APSTJ Global Education Seminar Workshop)(2013/05/25)
- Characteristic and kinetic analysis of gastrointestinal water dynamics: Impact on oral drug absorption(conference：DMPK Forum in Kanazawa)(2018/06/26)
- Multiple Binding Sites (MBS) Hypothesis: Substrate- and Dose-Dependent Drug Interactions Caused by Multiple Binding Sites on OATP2B1(conference：1st APSTJ Drug Interaction Focus Group Symposium)(2015/02/17)
- Inhibition of CYP2C19 and CYP3A4 by Omeprazole and its Metabolites(conference：18th International Conference on Cytochrome P450)(2013/06/19)
- Contribution of Metabolites to Drug-Drug Interactions(conference：36th JSCPT Annual Meeting)(2015/12/10)
- Mechanistic and Kinetic Analysis of Intestinal Drug Absorption Mediated by Transporters and Metabolic Enzymes(conference：30th JSSX Annual Meeting)(2015/11/13)
- Impact of CYP3A5 Expression on the Inhibition of CYP3A-Catalyzed Drug Metabolism: Considerations for Modeling CYP3A-Mediated Drug-Drug Interactions(conference：The 7th SUGIYAMA Laboratory (RIKEN) Open Symposium)(2016/02/16)
- Quantitative Analysis of the Impact of Controlled-Release Formulation on Oral Absorption and Bioavailability of P-gp/CYP3A4 Substrates(conference：4th International Symposium on BA/BE of Oral Drug Products (Session 3))(2022/11/18)
Others
- 1st Meeting on Oral Absorption Focus Group, Shizuoka Camp PHRM TECH JAPAN 27 7 2011/07
- Kinetic Analysis on Counteracting Interplay of Influx/Efflux Transporters on Intestinal Drug Absorption Drug Deliverly System 23 6 2008/06
- Quantitative Evaluation of the Effect of Transporters on Intestinal Absorption of Drugs Journal of Pharmaceutical Science and Technology, Japan 70 2 2010/02
Arts and Fieldwork
Patent
Theme to the desired joint research
Grant-in-Aid for Scientific Research
○「有機アニオン輸送体を介した薬物の肝移行性調節とその種差」(2007-2008)
○「吸収／排泄型トランスポーターの優位性に基づいた消化管吸収性評価システムの構築」(2008-2009)
○「細胞内オルガネラ膜トランスポーター活性の定量的可視化による薬物の細胞毒性評価」(2009-2010)
○「尿酸動態調節機構の解明に基づく血清尿酸値変動評価システムの樹立」(2009-2011)
○「部位差を考慮した薬物の消化管吸収性評価システムの構築とその製剤設計最適化への応用」(2011-2013)
○「尿酸結合タンパク質CD38を介した尿酸による炎症応答調節」(2023-2024)
○「Quantitative Evaluation of the Effect of Transporters and Metabolic Enzymes on Oral Drug Absorption」(2012-2013)
○「消化管水分調節機構の定量的解明に基づく薬物吸収動態予測システムの確立」(2020-2023)
○「小腸絨毛上皮モデルを用いた革新的in vitro薬物吸収性予測システムの開発」(2016-2018)
○「吸収/排泄型トランスポーターの相殺性に基づく薬物吸収動態予測」(2009-2010)