last modified：2025/04/03
Associate Professor Kobayashi, Isao
Faculty, Affiliation
Faculty of Biological Science and Technology,Institute of Science and Engineering
College and School Educational Field
Division of Natural Sciences, Graduate School of Natural Science and Technology
Course in Aquatic Biology and Environmental Science, School of Biological Science and Technology, College of Science and Engineering
Laboratory
Laboratory of Stem Cell Development TEL:076-264-6232 FAX:076-264-6232
Academic Background
【Academic background(Doctoral/Master's Degree)】
Nihon University Doctor Graduate School of Veterinary Medicine 2009 Completed
【Academic background(Bachelor's Degree)】
Nihon University Department of Veterinary Medicine 2006
【Degree】
Ph.D.
Career
Kanazawa University(2020/11-)
Kanazawa University(2018/04-2020/10)
Kanazawa University(2015/11-2018/03)
University of California, San Diego Department of Molecular Medicine(2015/04-2015/10)
University of California, San Diego Department of Molecular Medicine(2013/04-2015/03)
University of California, San Diego Department of Molecular Medicine(2011/10-2013/03)
Keio University(2009/04-2011/09)
Nihon University Graduate School of Veterinary Medicine(2008/04-2009/03)
Year & Month of Birth
1980/08
Academic Society
International Society for Experimental Hematology (ISEH)
Award
○Christa Muller-Sieburg Award(2013/08)
Specialities
Stem Cell Biology、
Speciality Keywords
Hematopoietic stem cell,Zebrafish,Stem cell niche,Angioblast,Osteoclast
Research Themes
Identification and Characterization of Hematopoietic Stem Cell Niche in The Fish Kidney
The homeostasis of the blood system is maintained by rare populations of multipotent hematopoietic stem cells (HSCs). HSCs are by far the most widely utilized human stem cells in the clinic in order to treat blood-related disorders, such as leukemia. HSCs are maintained by interactions with cells comprising their microenvironmental niche, which is established in the bone marrow in mammals. Despite the similarity of blood cell subsets, HSCs in teleost fish reside in the urinary organ, kidney, representing a very different environment relative to mammals. We will utilize comparative approaches for the study of HSC niches between zebrafish and mammals. Our ultimate goal is to define the required core components that regulate HSC dormancy, proliferation, and differentiation.
Elucidation of Regulatory Mechanisms Underlying Hematopoietic Stem Cell Development
A major goal of regenerative medicine is to instruct tissue-specific stem cells from induced pluripotent stem cells (iPSCs). Despite decades of efforts, however, generation of HSCs from iPSCs in vitro is not currently possible, indicating that an improved understanding of the native mechanisms of embryonic HSC specification is required. The zebrafish is a powerful genetic model for the study of HSCs. Its transparent embryos allow us to visualize the dynamics of developing HSCs using cell-specific transgenic animals. The movie shown here represents migration of angioblasts (green) along the ventral surface of somites (red). Utilizing “live-imaging” approaches in the zebrafish embryo, we will investigate when, where, and how HSC precursors are regulated by their environmental cells to establish HSC fate during embryogenesis.
Books
- Hirata, Hiromi,Iida, Atsuo Zebrafish, Medaka, and other small fishes : new model animals in biology, medicine, and beyond Springer Nature Singapore 2018
Papers
- Extremely low-frequency electromagnetic fields facilitate both osteoblast and osteoclast activity through Wnt/β-catenin signaling in the zebrafish scale Jingjing Kobayashi-Sun,Isao Kobayashi,Makoto Kashima,Jun Hirayama,Makiko Kakikawa,Sotoshi Yamada,Nobuo Suzuki Frontiers in Cell and Developmental Biology 12 2024/02/07
- Super-enhancer trapping by the nuclear pore via intrinsically disordered regions of proteins in squamous cell carcinoma cells. Masaharu Hazawa,Dini Kurnia Ikliptikawati,Yuki Iwashima,De-Chen Lin,Yuan Jiang,Yujia Qiu,Kei Makiyama,Koki Matsumoto,Akiko Kobayashi,Goro Nishide,Lim Keesiang,Hironori Yoshino,Toshinari Minamoto,Takeshi Suzuki,Isao Kobayashi,Makiko Meguro-Horike,Yan-Yi Jiang,Takumi Nishiuchi,Hiroki Konno,H Phillip Koeffler,Kazuyoshi Hosomichi,Atsushi Tajima,Shin-Ichi Horike,Richard W Wong Cell Chemical Biology in press 2023/11
- LSD1 promotes the egress of hematopoietic stem and progenitor cells into the bloodstream during the endothelial-to-hematopoietic transition Junya Tamaoki,Hiroki Maeda,Isao Kobayashi,Miki Takeuchi,Ken Ohashi,Aniket Gore,Florian Bonkhofer,Roger Patient,Brant M. Weinstein,Makoto Kobayashi Developmental Biology 501 92 2023/09
- The utility of zebrafish cardiac arrhythmia model to predict the pathogenicity of KCNQ1 variants Shihe Cui,Kenshi Hayashi,Isao Kobayashi,Kazuyoshi Hosomichi,Akihiro Nomura,Ryota Teramoto,Keisuke Usuda,Hirofumi Okada,Yaowen Deng,Jingjing Kobayashi-Sun,Tetsuo Nishikawa,Hiroshi Furusho,Takekatsu Saito,Hiroaki Hirase,Kunio Ohta,Manabu Fujimoto,Yuki Horita,Takashi Kusayama,Toyonobu Tsuda,Hayato Tada,Takeshi Kato,Soichiro Usui,Kenji Sakata,Noboru Fujino,Atsushi Tajima,Masakazu Yamagishi,Masayuki Takamura Journal of Molecular and Cellular Cardiology 177 50 2023/04
- The sinusoidal hematopoietic niche is formed by Jam1a via Notch signaling in the zebrafish kidney Mao Kondo,Koki Kimura,Jingjing Kobayashi-Sun,Shiori Yamamori,Makoto Taniguchi,David Traver,Isao Kobayashi iScience 26 4 106508 2023/03
- Inhibition of Canonical Wnt Signaling Promotes Ex Vivo Maintenance and Proliferation of Hematopoietic Stem Cells in Zebrafish. Koki Kimura,Shiori Yamamori,Masaharu Hazawa,Jingjing Kobayashi-Sun,Mao Kondo,Richard W Wong,Isao Kobayashi Stem cells (Dayton, Ohio) 40 9 831 2022/09/26
- Activation of RANKL-producing cells under simulated microgravity with a three-dimensional clinostat in regenerating goldfish scales Tatsuki Yamamoto,Mika Ikegame,Kohei Kuroda,Jingjing Kobayashi-Sun,Jun Hirayama,Isao Kobayashi,Ryoya Kawamura,Masato Endo,Yoshiaki Tabuchi,Yukihiro Furusawa,Koji Yachiguchi,Toshio Sekiguchi,Hajime Matsubara,Sachiko Yano,Atsuhiko Hattori,Nobuo Suzuki Biological Sciences in Space 36 9 2022/07 ?
- Hematopoietic Cell Isolation by Antibody-Free Flow Cytometry in the Zebrafish Embryo. Katsuhiro Konno,Jingjing Kobayashi-Sun,Fumio Arai,Isao Kobayashi,Daisuke Sugiyama Methods in molecular biology (Clifton, N.J.) 2520 171 2022/05/17
- Jagged 2b induces intercellular signaling within somites to establish hematopoietic stem cell fate in zebrafish. Yukino Wada,Hikaru Tsukatani,Chihiro Kuroda,Yurika Miyazaki,Miku Otoshi,Isao Kobayashi Development (Cambridge, England) 149 7 2022/04/01
- Non-aqueous, zwitterionic solvent as an alternative for dimethyl sulfoxide in the life sciences Kosuke Kuroda,Tetsuo Komori,Kojiro Ishibashi,Takuya Uto,Isao Kobayashi,Riki Kadokawa,Yui Kato,Kazuaki Ninomiya,Kenji Takahashi,Eishu Hirata Communications Chemistry 3 1 2020/11/11
- Impact of functional studies on exome sequence variant interpretation in early-onset cardiac conduction system diseases. Kenshi Hayashi,Ryota Teramoto,Akihiro Nomura,Yoshihiro Asano,Manu Beerens,Yasutaka Kurata,Isao Kobayashi,Noboru Fujino,Hiroshi Furusho,Kenji Sakata,Kenji Onoue,David Y Chiang,Tuomas O Kiviniemi,Eva Buys,Patrick Sips,Micah L Burch,Yanbin Zhao,Amy E Kelly,Masanobu Namura,Yoshihito Kita,Taketsugu Tsuchiya,Bunji Kaku,Kotaro Oe,Yuko Takeda,Tetsuo Konno,Masaru Inoue,Takashi Fujita,Takeshi Kato,Akira Funada,Hayato Tada,Akihiko Hodatsu,Chiaki Nakanishi,Yuichiro Sakamoto,Toyonobu Tsuda,Yoji Nagata,Yoshihiro Tanaka,Hirofumi Okada,Keisuke Usuda,Shihe Cui,Yoshihiko Saito,Calum A MacRae,Seiji Takashima,Masakazu Yamagishi,Masa-Aki Kawashiri,Masayuki Takamura Cardiovascular research 116 13 2116 2020/11/01
- Melatonin suppresses both osteoblast and osteoclast differentiation through repression of epidermal Erk signaling in the zebrafish scale. Jingjing Kobayashi-Sun,Nobuo Suzuki,Atsuhiko Hattori,Masaaki Yamaguchi,Isao Kobayashi Biochemical and biophysical research communications 530 4 644 2020/10/01
- Uptake of osteoblast-derived extracellular vesicles promotes the differentiation of osteoclasts in the zebrafish scale Jingjing Kobayashi-Sun,Shiori Yamamori,Mao Kondo,Junpei Kuroda,Mika Ikegame,Nobuo Suzuki,Kei ichiro Kitamura,Atsuhiko Hattori,Masaaki Yamaguchi,Isao Kobayashi Communications Biology 3 1 2020/04/23
- Dual role of Jam3b in early hematopoietic and vascular development Isao Kobayashi,Jingjing Kobayashi-Sun,Yuto Hirakawa,Madoka Ouchi,Koyuki Yasuda,Hiroyasu Kamei,Shigetomo Fukuhara,Masaaki Yamaguchi Development (Cambridge) 147 1 2020/01/08
- Enrichment of hematopoietic stem/progenitor cells in the zebrafish kidney. Isao Kobayashi,Mao Kondo,Shiori Yamamori,Jingjing Kobayashi-Sun,Makoto Taniguchi,Kaori Kanemaru,Fumihiko Katakura,David Traver Scientific reports 9 1 14205 2019/10/02
- Rap1b Promotes Notch-Signal-Mediated Hematopoietic Stem Cell Development by Enhancing Integrin-Mediated Cell Adhesion. Seung-Sik Rho,Isao Kobayashi,Eri Oguri-Nakamura,Koji Ando,Masakazu Fujiwara,Naomi Kamimura,Hiromi Hirata,Atsuo Iida,Yoshiko Iwai,Naoki Mochizuki,Shigetomo Fukuhara Developmental cell 49 5 681 2019/06/03
- Anteroposterior molecular registries in ectoderm of the echinus rudiment Shinya Adachi,Iyo Niimi,Yui Sakai,Fuminori Sato,Takuya Minokawa,Makoto Urata,Atsuko Sehara-Fujisawa,Isao Kobayashi,Masaaki Yamaguchi Developmental Dynamics 247 12 1297 2018/11/05 ?
- RANKL, Ephrin-Eph and Wnt10b are key intercellular communication molecules regulating bone remodeling in autologous transplanted goldfish scales Yuya Tazaki,Kayo Sugitani,Kazuhiro Ogai,Isao Kobayashi,Haruki Kawasaki,Takafumi Aoyama,Nobuo Suzuki,Yoshiaki Tabuchi,Atsuhiko Hattori,Kei ichiro Kitamura Comparative Biochemistry and Physiology -Part A : Molecular and Integrative Physiology 225 46 2018/06/15
- Effects of low-intensity pulsed ultrasound on osteoclasts: Analysis with gold-fish scales as a model of bone Taizo Hanmoto,Yoshiaki Tabuchi,Mika Ikegame,Takashi Kondo,Kei-ichiro Kitamura,Masato Endo,Isao Kobayashi,Hiroyuki Mishima,Toshio Sekiguchi,Makoto Urata,Azusa Seki,Sachiko Yano,Atsuhiko Hattori,Nobuo Suzuki BIOMEDICAL RESEARCH-TOKYO 38 1 71 2017/02/01
- Effects of hyperglycemia on bone metabolism and bone matrix in goldfish scales Kei-ichiro Kitamura,Tadashi Andoh,Wakana Okesaku,Yuya Tazaki,Kazuhiro Ogai,Kayo Sugitani,Isao Kobayashi,Nobuo Suzuki,Wenxi Chen,Mika Ikegame,Atsuhiko Hattori COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY 203 152 2017/01
- Isolation and characterization of hematopoietic stem cells in teleost fish Isao Kobayashi,Fumihiko Katakura,Tadaaki Moritomo DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 58 86 2016/05
- Gata2b is a restricted early regulator of hemogenic endothelium in the zebrafish embryo Emerald Butko,Martin Distel,Claire Pouget,Bart Weijts,Isao Kobayashi,Kevin Ng,Christian Mosimann,Fabienne E. Poulain,Adam McPherson,Chih-Wen Ni,David L. Stachura,Natasha Del Cid,Raquel Espin-Palazon,Nathan D. Lawson,Richard Dorsky,Wilson K. Clements,David Traver DEVELOPMENT 142 6 1050 2015/03
- Jam1a-Jam2a interactions regulate haematopoietic stem cell fate through Notch signalling Isao Kobayashi,Jingjing Kobayashi-Sun,Albert D. Kim,Claire Pouget,Naonobu Fujita,Toshio Suda,David Traver NATURE 512 7514 319 2014/08/21
- Kinetics of CD4(+) and CD8 alpha(+) T-cell subsets in graft-versus-host reaction (GVHR) in ginbuna crucian carp Carassius auratus langsdorfii Yasuhiro Shibasaki,Flideaki Toda,Isao Kobayashi,Tadaaki Moritomo,Teruyuki Nakanishi DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 34 10 1075 2010/10
- Isolation and characterization of endosteal niche cell populations that regulate hematopoietic stem cells Yuka Nakamura,Fumio Arai,Hiroko Iwasaki,Kentaro Hosokawa,Isao Kobayashi,Yumiko Gomei,Yoshiko Matsumoto,Hiroki Yoshihara,Toshio Suda BLOOD 116 9 1422 2010/09/02
- Comparative gene expression analysis of zebrafish and mammals identifies common regulators in hematopoietic stem cells. Kobayashi I,Ono H,Moritomo T,Kano K,Nakanishi T,Suda T Blood 115 2 e1 2010/01/14
- In vivo characterization of primitive hematopoietic cells in clonal ginbuna crucian carp (Carassius auratus langsdorfii) Isao Kobayashi,Hiroko Kusakabe,Hideaki Toda,Tadaaki Moritomo,Tomoko Takahashi,Teruyuki Nakanishi VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY 126 1-2 74 2008/11/15
- Characterization of teleost phagocyte NADPH oxidase: Molecular cloning and expression analysis of carp (Cyprinus carpio) phagocyte NADPH oxidase Masayuki Mayumi,Yoko Takeda,Masataka Hoshiko,Ken Serada,Michihiro Murata,Tadaaki Moritomo,Furnio Takizawa,Isao Kobayashi,Kyosuke Araki,Teruyuki Nakanishi,Hideki Sumimoto MOLECULAR IMMUNOLOGY 45 6 1720 2008/03
- Long-term hematopoietic reconstitution by transplantation of kidney hematopoietic stem cells in lethally irradiated clonal ginbuna crucian carp (Carassius auratus langsdorfii) Isao Kobayashi,Sawako Kuniyoshi,Kazuyuki Saito,Tadaaki Moritomo,Tomoko Takahashi,Teruyuki Nakanishi DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 32 8 957 2008/02/15
- Characterization and localization of side population (SP) cells in zebrafish kidney hematopoietic tissue Isao Kobayashi,Kazuyuki Saito,Tadaaki Moritomo,Kyosuke Araki,Fumio Takizawa,Teruyuki Nakanishi BLOOD 111 3 1131 2008/02/01
- Molecular cloning and expression analysis of T-bet in ginbuna crucian carp (Carassius auratus langsdorfii) Fumio Takizawa,Kyosuke Araki,Isao Kobayashi,Tadaaki Moritomo,Mitsuru Ototake,Teruyuki Nakanishi MOLECULAR IMMUNOLOGY 45 1 127 2008/01
- Isolation of side population cells from ginbuna carp (Carassius auratus langsdorfii) kidney hematopoietic tissues Isao Kobayashi,Tadaaki Moritomo,Mituru Ototake,Teruyuki Nakanishi DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 31 7 696 2007/11/13
- Demonstration of hematopoietic stem cells in ginbuna carp (Carassius auratus langsdorfii) kidney Isao Kobayashi,Mayuko Sekiya,Tadaaki Moritomo,Mituru Ototake,Teruyuki Nakanishi DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 30 11 1034 2006/02/20
Conference Presentations
- Developing hematopoietic stem cells acquire adaptability to the final hematopoietic niche(2022/06/01)
- 母性由来Abcg2aは背側化を抑制して造血・血管発生を促進する(2021/12/02)
- Jagged-2bは体節内細胞間シグナル伝達を介して造血幹細胞の発生を制御する(2021/12/01)
- ゼブラフィッシュにおいて古典的Wntシグナルの阻害は造血幹細胞を維持する(2021/12/01)
- Inhibition of canonical Wnt signaling maintains hematopoietic stem cells in zebrafish(2021/09/16)
- Jagged-2b triggers intercellular signaling in the somite to establish the hematopoietic stem cell fate in zebrafish(conference：The 50th Annual Scientific Meeting of the ISEH-Society for Hematology and Stem Cells)(2021/08/25)
- Sinusoidal endothelium is a key component of hematopoietic niches in the zebrafish kidney(2020/12/02)
- Developing hematopoietic stem cells acquire adaptability to the final hematopoietic niche(2020/11/20)
- Identification of hematopoietic stem cell niches in the zebrafish kidney(2020/02/14)
- ゼブラフィッシュ鱗における骨芽細胞由来の細胞外小胞を取り込みは破骨細胞の分化を促進する(2019/12/08)
- Jag2bは体節においてEphrin A1b – Wnt16を介して造血幹細胞の発生を制御する(2019/12/07)
- ゼブラフィッシュにおいてIntegrinはlrrc15を介して造血幹細胞発生を制御する(2019/12/07)
- ゼブラフィッシュ腎臓における造血ニッチの同定(2019/12/07)
- 発生期の造血幹細胞は最終的な造血環境への適応能を獲得する(2019/12/06)
- ゼブラフィッシュにおいてIntegrinはlrrc15を介して造血幹細胞の発生を制御する(2019/12/06)
- Uptake of osteoblast-derived extracellular vesicles facilitates the differentiation of osteoclasts in the zebrafish scale(2019/12/05)
- Uptake of osteoblast-derived extracellular vesicles facilitates the differentiation of osteoclasts in the zebrafish scale(2019/09/05)
- The Sinusoidal Endothelium Functions as a Hematopoietic Niche in the Zebrafish Kidney(conference：The 48th Annual Scientific Meeting of the ISEH-Society for Hematology and Stem Cells)(2019/08/23)
- 血液・血管系の発生機構の解明を目指して(2019/05/14)
- ゼブラフィッシュ鱗を用いた骨折修復過程におけるライブイメージング解析(2018/11/29)
- jam1a変異ゼブラフィッシュを用いた造血幹細胞の維持・増殖機構の解明(2018/11/28)
- ゼブラフィッシュ鱗を用いた骨折修復過程におけるライブイメージング解析(2018/09/14)
- jam1a変異ゼブラフィッシュを用いた造血幹細胞の維持・増殖機構の解明(2018/09/13)
- Jam3b regulates hemato-vascular development through the repression of the Erk signaling pathway(conference：The 47th Annual Scientific Meeting of the ISEH-Society for Hematology and Stem Cells)(2018/08/25)
- 小型魚類の造血システムに学ぶ幹細胞研究の最前線(2017/09/22)
- Identification of Novel Regulatory Molecules for Hemato-vascular Development in Zebrafish(2017/08/30)
- Jam1aおよび Jam2aによる接着は造血幹細胞の発生に必須な Notchのシグナル伝達を促進する(2016/12/01)
- The Somitic Notch Signaling Regulates Hematopoietic Stem Cell Specification(2016/10/06)
- A Direct Interaction with The Somite Regulates Hematopoietic Stem Cell Fate(2016/08/21)
- Cell-to-cell interaction between endothelial-hematopoietic precursors and somites regulates developmental specification of hematopoietic stem cells through Notch signaling(conference：The 42nd Annual Scientific Meeting of the ISEH-Society for Hematology and Stem Cells)(2013/08/24)
Others
Arts and Fieldwork
Patent
Theme to the desired joint research
Grant-in-Aid for Scientific Research
○「造血幹細胞の形成過程におけるインテグリン由来シグナル伝達経路の役割解明」(2019-2021)
○「ウロコ骨折モデルによる破骨細胞産生新規カップリング因子の同定と分泌時相の解明」(2018-2020)
○「背側大動脈における造血幹細胞の発生動態の解析」(2017-2018)
○「栄養環境センサーを分子基軸とした脊椎側弯症に対する発症・進行予測技術の開発」(2017-2019)
○2022「骨芽細胞で作られるホルモンによる破骨細胞の調節機構：骨モデル（ウロコ）による解析」(2020-2022)
○「異種ドナー生殖細胞の生着・分化をサポートする新たな生殖細胞移植技術の開発」(2021-2023)
Competitive research funding,Contribution
Collaborative research,Consignment study
Classes (Bachelors)
○Developmental Biology B(2022)
○Developmental Biology C(2022)
○Developmental Biology Lab(2022)
○Experiments in Biology(2022)
○Biology for Education(2022)
○Basic Biology Lab 2(2022)
○Guide to Biological Science B(2022)
Classes (Graduate Schools)
○Developmental Genetics(2022)
○Genomic Systems Biology A(2022)