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Researcher Information

last modified：2024/03/18

Professor　SAKURAI Hiroshi

Mail

Faculty, Affiliation

Faculty of Health Sciences, Institute of Medical, Pharmaceutical and Health Sciences

College and School Educational Field

Division of Health Sciences, Graduate School of Medical Science
Division of Health Sciences，Graduate School of Medical Science
Department of Laboratory Sciences，School of Health Sciences, College of Medical, Pharmaceutical and Health Sciences

Laboratory

laboratory of life sciences　TEL:076-265-2588　FAX:076-234-4360

Academic Background

【Academic background(Doctoral/Master's Degree)】
Tokyo Metropolitan University　Doctor　Graduate School, Division of Natural Science　1990
【Academic background(Bachelor's Degree)】
Tokyo Metropolitan University　198503

Career

Keio University School of Medicine　laboratory of molecular genetics(1990/05/01-1996/03/31)
Kanazawa University　Faculty of Medicine(1996/04/01-1998/12/31)
Kanazawa University　Faculty of Medicine(1999/01/01-)

Year & Month of Birth

1963/03

Academic Society





Award

Specialities

Molecular biology

Speciality Keywords

Research Themes

Transcriptional regulation by yeast transcription factor GAL11

studies on mechanisms of stress response

Books

  • 　Transcription regulation in eukariotes　Human Frontier Science Program　1999/09

Papers

  • 　A genome-wide analysis of transcriptional effect of Gal11 in Saccharomyces cerevisiae.　DNA Research　8　1　1-9　2001/01
  • 　Activator-specific requirement for the general transcription factor IIE in yeast.　BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS　261　3　734-739　1999/08
  • 　Functional connections between Mediator components and general transcription factors of Saccharomyces cerevisiae.　The Journal of Biological Chemistry　275　47　37251-37256　2000/11
  • 　Functional Correlation among Gal11, TFIIE, and TFIIH in Saccharomyces cerevisiae. Gal11 and TFIIE cooperatively enhance TFIIH-mediated phosphorylation of RNA polymerase II carboxy-terminal domain sequences.　The Journal of Biological Chemistry　273　16　9534-9538　1998/04
  • 　Promoter structure-dependent functioning of the general transcription factor IIE in Saccharomyces cerevisiae.　The Journal of Biological Chemistry　272　25　15936-15942　1997/06

show all

  • 　Transcription initiation mediated by initiator binding protein in Saccharomyces cerevisiae.　BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS　255　1　157-163　1999/02
  • 　Yeast Gal11 and Transcription Factor IIE Function Through a Common Pathway in Transcriptional Regulation.　The Journal of Biological Chemistry　272　51　32663-32669　1997/12
  • 　Artificial recruitment of certain Mediator components affects requirement of basal transcription factor IIE.　GENES TO CELLS　8　1　41-51　2003/01
  • 　A novel domain of the yeast heat shock factor that regulates its activation function.　BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS　261　3　734-739　2001/09
  • 　Identification of a novel class of target genes and a novel type of binding sequence of heat shock transcription factor in Saccharomyces cerevisiae.　JOURNAL OF BIOLOGICAL CHEMISTRY　280　12　11911-11919　2005/03
  • 　Saccharomyces cerevisiae heat shock transcription factor regulates cell wall remodeling in response to heat shock.　Eukaryotic Cell　4　6　1050-1056　2005/06
  • 　Phosphorylation of the yeast heat shock transcription factor is implicated in gene-specific activation dependent on the architecture of the heat shock element.　MOLECULAR AND CELLULAR BIOLOGY　24　9　3648-3659　2004/05
  • 　Stress-induced transcription of the endoplasmic reticulum oxidoreductin gene ERO1 in the yeast Saccharomyces cerevisiae.　Takemori, Y., Sakaguchi, A., Matsuda, S., Mizukami, Y. and Sakurai, H.　MOLECULAR GENETICS AND GENOMICS　275　1　89-96　2006/01
  • 　Mutated yeast heat shock transcription factor activates transcription independently of hyperphosphorylation.　Hashikawa, N., Mizukami, Y., Imazu, H. and Sakurai, H.　JOURNAL OF BIOLOGICAL CHEMISTRY　281　7　3936-3942　2006/02
  • 　The DNA-binding domain of yeast Hsf1 regulates both DNA binding and transcriptional activities.　Yamamoto, A. and Sakurai H.　BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS　346　4　1324-1329　2006/08
  • 　Different mechanisms are involved in the transcriptional activation by heat shock transcription factor through different types of heat shock elements.　Hashikawa, N., Yanamoto, N., and Sakurai, H.　JOURNAL OF BIOLOGICAL CHEMISTRY　282　14　10333-10340　2007/04
  • 　Interaction between heat shock transcription factors (HSFs) and divergent binding sequences: binding specificities of yeast HSFs and human HSF1.　Sakurai, H. and Takemori, Y.　JOURNAL OF BIOLOGICAL CHEMISTRY　282　18　13334-13341　2007/05
  • 　Role of heat shock transcription factor in the Saccharomyces cerevisiae oxidative stress response.　Yamamoto, A., Ueda, J., Yamamoto, N., Hashikawa, N. and Sakurai, H.　Eukaryotic Cell　6　8　1373-1379　2007/08
  • 　HSF1 activation inhibits rapamycin resistance and TOR signaling in Yeast revealed by combined proteomic and genetic analysis.　Bandhakavi S, Xie H, O'Callaghan B, Sakurai H, Kim D, Griffin TJ　PLoS One　3　2　e1598　2008/02
  • 　Regulation of thermotolerance by stress-induced transcription factors in Saccharomyces cerevisiae.　Yamamoto N, Maeda Y, Ikeda A, Sakurai H　Eukaryotic Cell　7　5　783-790　2008/05
  • 　Differential recognition of heat shock elements by members of the heat shock transcription factor family.　Yamamoto N, Takemori Y, Sakurai M, Sugiyama K, Sakurai H　FEBS Journal　276　1962-1974　2009/04
  • 　Diversity in DNA recognition by heat shock transcription factors (HSFs) from model organisms. 　Enoki, Y. and Sakura H. 　FEBS LETTERS　585　1293-1298　2011/05
  • 　Binding affinities and interactions among different heat shock element types and heat shock factors in rice (Oryza sativa L.).　Mittal, D. J., Enoki, Y., Lavania, D., Singh, A., Sakurai, H. and Grover, A. 　FEBS Journal　2011/10
  • 　Novel aspects of heat shock factors: DNA recogniton, chromatin modulation and gene expression.　Sakurai, H. and Enoki, Y. 　FEBS Journal　277　4140-4149　2010/10
  • 　A unified nomenclature for protein subunits of Mediator complexes linking transcriptional regulators to RNA polymerase II.　MOLECULAR CELL　14　553-557　2004/06
  • 　Galactose transporters discriminate steric anomers at the cell surface in yeast.　Fukasawa T, Sakurai H, Nogi Y, Baruffini E　FEMS Yeast Research　9　723-732　2009/06
  • 　Carboxy-terminal region of the yeast heat shock factor contains two domains that make transcription independent of the TFIIH protein kinase.　Sakurai H., Hashikawa, N., Imazu, H. Fukasawa, T.　GENES TO CELLS　8　12　951-961　2003/12
  • 　In vitro transcription systems from cultured cells and fat body tissue of the silkworm, Bombyx mori.　MOLECULAR BIOTECHNOLOGY　8　79-88　1997/08
  • 　DNA-binding and transcriptional activities of human HSF4 containing mutations that associate with congenital and age-related cataracts.　Enoki, Y., Mukoda, Y., Furutani, C. and Sakurai, H. 　BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE　1802　749-753　2010/06
  • 　Mutational analysis of human heat-shock transcription factor 1 reveals a regulatory role for oligomerization in DNA-binding specificity. 　Takemori, Y., Enoki, Y., Yamamoto, N., Fukai, Y., Adachi, K. and Sakurai, H.　BIOCHEMICAL JOURNAL　424　253-261　2009/12
  • 　Regulation of chaperone gene expression by heat shock transcription factor in Saccharomyces cerevisiae: Importance in normal cell growth, stress resistance, and longevity　Sakurai, H. and Ota, A. 　FEBS LETTERS　2011/09
  • 　Stress-induced transcription of regulator of G protein signaling 2 (RGS2) by heat shock transcription factor HSF1.　BIOCHIMIE　95　1432-1436　2013/04
  • 　Evolutionarily conserved domain of heat hock transcription factor negatively regulates oligomerization and DNA binding.　Ota, A., Sawai, M. and Sakurai, H.　BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION　1829　9　930-936　2013/09
  • 　Dynamic regulation of extracellular signal-regulated kinase (ERK) by protein phosphatase 2A regulatory subunit B56gamma1 in nuclei induces cell migration.　PLoS One　8　5　e63727 　2013/05
  • 　PP2A and its adapter protein IER5 induce the DNA-binding ability and target gene expression of E2F1 via dephosphorylation at serine 375　Hiroto Takeuchi, Mayuko Koga, Kuriko Doi, Hiroshi Sakurai　Biochim Biophys Acta Gene Regul Mech　1866　3　194960　2023/09
  • 　PP2A-B55 and its adapter proteins IER2 and IER5 regulate the activity of RB family proteins and the expression of cell cycle-related genes.　Doi, K., Takeuchi, H. and Sakurai, H.　FEBS Journal　290　2　745-762　2023/02
  • 　IER family proteins are regulators of protein phosphatase PP2A and modulate the phosphorylation status of CDC25A.　Ueda T, Kohama Y, Sakurai H.　Cellular Signalling　55　81-89　2019/03
  • 　Regulation of the stability and activity of CDC25A and CDC25B by protein phosphatase PP2A and 14-3-3 binding.　Kohama Y, Saito M, Yada M, Sakurai H.　Cellular Signalling　54　10-16　2019/02
  • 　TAF7 is a heat-inducible unstable protein and is required for sustained expression of heat shock protein genes.　Nagashimada M, Ueda T, Ishita Y, Sakurai H　FEBS Journal 　285　3215-3224　2018/09
  • 　GADD45 family proteins suppress JNK signaling by targeting MKK7. 　Ueda T, Kohama Y, Kuge A, Kido E, Sakurai H　Archives of Biochemistry and Biophysics　635　1-7　2017/12/01
  • 　Activation of ERK/IER3/PP2A-B56 positive feedback loop in lung adenocarcinoma by allelic deletion of B56 gene.　Ito, T., Ozaki, S., Chanasong, R., Mizutani, Y., Oyama, T., Sakurai, H., Matsumoto, I., Takemura, H. and Kawahara, E.　Oncology Reports 　35　5　2635-2642　2016/05/01
  • 　Heme orientation of cavity mutant hemoglobins (F8His→Gly) in either  or  subunits: Circular dichroism, 1H NMR, and resonance Raman studies. 　Nagai, M., Nagai, Y., Aki, Y., Sakurai, H., Mizusawa, N., Ogura, T., Kitagawa, T., Yamamoto, Y. and Nagatomo, S.　Chirality 　28　8　585-592　2016/08
  • 　HSF1 transcriptional activity is modulated by IER5 and PP2A/B55.　Ishikawa Y, Kawabata S, Sakurai H.　FEBS Letters 　589　1150-1155　2015/06
  • 　An origin of cooperative oxygen binding of human adult hemoglobin: different roles of the  and  subunits in the 22 tetramer.　Nagatomo, S., Nagai, Y., Aki, Y., Sakurai, H., Imai, K., Mizusawa, N., Ogura, T., Kitagawa, T. and Nagai, M.　PLoS One 　10　e0135080　2015/04
  • 　Involvement of propionate side chains of the heme in circular dichroism of myoglobin: Experimental and theoretical analysis.　Nagai, M., Kobayashi, C., Nagai, Y., Imai, K., Mizusawa, H., Sakurai, H., Neya, S., Kayanuma, M., Shoji, M. and Nagatomo, S.　J. Phys. Chem. B　119　4　1275-1287　2015/01
  • 　Heat-induced expression of the immediate-early gene IER5 and its involvement in the proliferation of heat-shocked cells.　Ishikawa Y, Sakurai H.　FEBS Journal 　282　332-340　2015/01
  • 　Heat shock transcription factor HSF1 regulates the expression of the Huntingtin-interacting protein HYPK.　Sakurai H, Sawai M, Ishikawa Y, Ota A, Kawahara E　Biochim. Biophys. Acta　1840　1181-1187　2014/04
  • 　The proto-oncogene JUN is a target of the heat shock transcription factor HSF1.　Sawai M, Ishikawa Y, Ota A, Sakurai H.　FEBS Journal 　280　6672-6680　2013/12
  • 　Immediate-early response 5 (IER5) interacts with protein phosphatase 2A and regulates the phosphorylation of ribosomal protein S6 kinase and heat shock factor 1.　Kawabata S, Ishita Y, Ishikawa Y, Sakurai H.　FEBS Letters 　589　3679-3685　2015/10

Conference Presentations

  • Functional regulation of transcription factor E2F1 by phosphorylation(2022/12/01)
  • Protein phosphatase PP2A adapter proteins regulate phosphorylation and activity of Rb.(2021/12/02)
  • Regulation of cell cycle gene expression by protein phosphatase 2A and its adaptor proteins.(2020/12/02)
  • Heat shock response and MAP kinase regulation by growth arrest and DNA damage-inducible GADD45(2016/11/30)

Others

Arts and Fieldwork

Patent

Theme to the desired joint research

○development of new techniques for molecular biology

Grant-in-Aid for Scientific Research

○「基本転写因子のプロモーター構造特異的な作用機構」(1997-)　
○「転写調節における基本転写因子とプロモーターの特異的相互作用」(1998-)　
○「基本転写因子のプロモーター構造特異的な作用機構」(1998-)　
○「転写調節因子およびプロモーター構造に特異的な転写開始機構」(1999-)　
○「転写開始から伸長反応への移行における転写因子の作用機構」(1999-)　
○「転写開始複合体を形成するタンパク質間の遺伝子特異的な相互作用」(2000-)
○「転写開始複合体を形成するタンパク質間の遺伝子特異的な相互作用」(2000-)　
○「転写開始から伸長反応への移行における転写因子の作用機構」(2000-)　
○「熱ショック転写因子と転写開始複合体の相互作用」(2001-)　
○「熱ショック転写因子による多様な塩基配列の認識と生物間での普遍性」(2007-2008)　
○「ストレス応答における熱ショック転写因子のリン酸化制御」(2004-)　
○「熱ショック転写因子による多様な塩基配列の認識と生物間での普遍性」(2007-)　
○「熱ショック転写因子による遺伝子特異的な転写制御機構」(2009-)
○「運動と非アルコール性脂肪肝炎の改善をつなぐ制御分子機構の解明」(2022-2025)　
○2022「プロテインホスファターゼPP2Aによるシグナル伝達と細胞増殖の制御」(2020-2022)　
○「タンパク質ミスフォールディングに注目したうつ病発症メカニズムの解明」(2019-2021)　
○「熱ショック転写因子によるシャペロン非依存的恒常性維持機構の解明」(2016-2018)　
○「熱ショック転写因子による遺伝子特異的な転写制御機構」(2009-2011)　
○「熱ショック転写因子によるシャペロン非依存的恒常性維持機構の解明」(2016-2018)　

Competitive research funding,Contribution

Collaborative research,Consignment study

Classes (Bachelors)

○Gene Diagnostics(2023)
○Molecular Biology(2023)
○Biochemistry(2023)
○Molecular Biology(2023)
○Experiments in Biochemistry(2023)
○Gene Diagnostics(2023)
○Biochemistry(2023)
○Biochemistry(2023)
○Biological Chemistry Practice(2023)
○Biochemistry(2023)
○Biological Chemistry Practice(2023)
○Molecular and Cellular Biology(2023)
○Introduction to Data Science(2023)
○Molecular and Cellular Biology(2023)
○Molecular and Cellular Biology(2022)
○Molecular and Cellular Biology(2022)
○Introduction to Data Science(2022)
○Exercise on Recombinant DNA Technology(2022)
○Gene Diagnostics(2022)
○Biochemistry(2022)
○Exercise on Recombinant DNA Technology(2022)
○Biochemistry(2022)
○Gene Diagnostics(2022)
○Experiments in Biochemistry(2022)
○Molecular Biology Lecture and Practice(2022)
○Genetics(2022)
○Experiments in Life Science(2022)
○Experiments in Life Science(2022)
○Biochemistry(2022)
○Molecular and Cellular Biology(2021)
○Introduction to Data Science(2021)
○Molecular and Cellular Biology(2021)
○Gene Diagnostics(2021)
○Genetics(2021)
○Experiments in Biochemistry(2021)
○Biochemistry(2021)
○Experiments in Life Science(2021)
○Experiments in Life Science(2021)
○Biochemistry(2021)
○Molecular Biology Lecture and Practice(2021)
○Exercise on Recombinant DNA Technology(2021)
○Biochemistry(2020)
○Experiments in Biochemistry(2020)
○Molecular Biology Lecture and Practice(2020)
○Exercise on Recombinant DNA Technology(2020)
○Genetics(2020)
○Biochemistry(2020)
○Experiments in Life Science(2020)
○Experiments in Life Science(2020)
○Experiments in Life Science(2020)
○Experiments in Life Science(2020)
○Gene Diagnostics(2020)
○Gene Diagnostics(2020)
○Clinial Practice of Clinical Chemistry(2020)
○Introduction to Information Technology(2020)
○Molecular and Cellular Biology(2020)
○Molecular and Cellular Biology(2020)
○Molecular and Cellular Biology(2020)

Classes (Graduate Schools)

○Advanced Laboratory Oncology: Research(2023)
○Advanced Laboratory Oncology: Research(2023)
○Advanced Laboratory Oncology: Research(2023)
○Laboratory Oncology: Seminar(2023)
○Advanced Laboratory Oncology: Research(2023)
○Laboratory Oncology: Seminar(2023)
○Advanced Laboratory Oncology: Research(2023)
○Advanced Laboratory Oncology: Research(2023)
○Laboratory Oncology: Seminar(2023)
○Advanced Laboratory Oncology: Research(2023)
○Laboratory Oncology: Seminar(2023)
○Advanced Laboratory Oncology: Research(2023)
○Advanced Laboratory Oncology: Research(2023)
○Advanced Laboratory Oncology: Research(2023)
○Advanced Laboratory Oncology: Research(2023)
○Advanced Laboratory Oncology: Research(2023)
○Laboratory Oncology: Lecture(2023)
○Laboratory Oncology: Lecture(2023)
○Advanced Laboratory Oncology: Research(2023)
○Advanced Laboratory Oncology: Research(2023)
○Advanced Laboratory Oncology: Research(2023)
○Advanced Laboratory Oncology: Research(2023)
○Laboratory Oncology: Lecture(2023)
○Advanced Laboratory Oncology: Research(2023)
○Laboratory Oncology: Lecture(2023)
○Advanced Laboratory Oncology: Research(2023)
○Laboratory Rotation I(2023)
○Molecular Biology of Tumor: Seminar(2023)
○Laboratory Oncology: Research(2023)
○Laboratory Oncology: Research(2023)
○Laboratory Oncology: Research(2023)
○Laboratory Oncology: Research(2023)
○Laboratory Oncology: Research(2023)
○Laboratory Oncology: Research(2023)
○Molecular Biology of Tumor: Seminar(2023)
○Laboratory Oncology: Research(2023)
○Laboratory Oncology: Research(2023)
○Laboratory Oncology: Research(2023)
○Laboratory Oncology: Research(2023)
○Laboratory Oncology: Research(2023)
○Molecular Biology of Tumor: Seminar(2023)
○Molecular Biology of Tumor: Seminar(2023)
○Laboratory Oncology: Research(2023)
○Laboratory Rotation Ⅱ(2023)
○Laboratory Rotation Ｉ(2023)
○Laboratory Oncology: Research(2023)
○Laboratory Oncology: Research(2023)
○Laboratory Oncology: Research(2023)
○Laboratory Oncology: Research(2023)
○Molecular Biology of Tumor: Lecture(2023)
○Molecular Biology of Tumor: Lecture(2023)
○An Introduction to Medical Laboratory Sciences:Lec(2023)
○An Introduction to Medical Laboratory Sciences:Lec(2023)
○Molecular Biology of Tumor: Lecture(2023)
○Laboratory Oncology: Research(2023)
○Laboratory Oncology: Research(2023)
○Laboratory Oncology: Research(2023)
○Laboratory Oncology: Research(2023)
○Molecular Biology of Tumor: Lecture(2023)
○Laboratory RotationⅠ(2023)
○Laboratory RotationⅠ(2023)
○Laboratory RotationⅠ(2023)
○Laboratory Rotation I(2023)
○Laboratory Rotation I(2023)
○Advanced Laboratory Oncology: Research(2022)
○Advanced Laboratory Oncology: Research(2022)
○Laboratory Oncology: Seminar(2022)
○Advanced Laboratory Oncology: Research(2022)
○Laboratory Oncology: Seminar(2022)
○Advanced Laboratory Oncology: Research(2022)
○Laboratory Oncology: Lecture(2022)
○Advanced Laboratory Oncology: Research(2022)
○Advanced Laboratory Oncology: Research(2022)
○Advanced Laboratory Oncology: Research(2022)
○Advanced Laboratory Oncology: Research(2022)
○Advanced Laboratory Oncology: Research(2022)
○Advanced Laboratory Oncology: Research(2022)
○Laboratory Oncology: Lecture(2022)
○Advanced Laboratory Oncology: Research(2022)
○Advanced Laboratory Oncology: Research(2022)
○Laboratory Oncology: Research(2022)
○Molecular Biology of Tumor: Seminar(2022)
○Laboratory Oncology: Research(2022)
○Molecular Biology of Tumor: Seminar(2022)
○Laboratory Oncology: Research(2022)
○Introduction to Health Science: Lecture(2022)
○Molecular Biology of Tumor: Lecture(2022)
○An Introduction to Medical Laboratory Sciences:Lec(2022)
○Laboratory Oncology: Research(2022)
○Molecular Biology of Tumor: Lecture(2022)
○Advanced Laboratory Oncology: Research(2021)
○Laboratory Oncology: Seminar(2021)
○Laboratory Oncology: Lecture(2021)
○Introduction to Health Science: Lecture(2021)
○Laboratory Oncology: Research(2021)
○Molecular Biology of Tumor: Seminar(2021)
○Molecular Biology of Tumor: Lecture(2021)
○An Introduction to Medical Laboratory Sciences:Lec(2021)
○Laboratory Oncology: Seminar(2020)
○Advanced Laboratory Oncology: Research(2020)
○Laboratory Oncology: Lecture(2020)
○Introduction to Health Science: Lecture(2020)
○Molecular Biology of Tumor: Seminar(2020)
○Laboratory Oncology: Research(2020)
○Molecular Biology of Tumor: Lecture(2020)
○An Introduction to Medical Laboratory Sciences:Lec(2020)
○Laboratory Oncology: Research(2016)
○An Introduction to Medical Laboratory Sciences:Lec(2016)
○Laboratory Oncology: Seminar(2016)
○Advanced Laboratory Oncology: Research(2016)
○Laboratory Oncology: Lecture(2016)
○Molecular Biology of Tumor: Lecture(2016)
○Molecular Biology of Tumor: Seminar(2016)

International Project

International Students

Lecture themes

Others (Social Activities)

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