KANEMORI Masaaki - Directory of Researchers at Kanazawa University

Faculty, Affiliation

Faculty of Biological Science and Technology,Institute of Science and Engineering 　Functional Biochemistry

College and School Educational Field

Division of Natural Sciences, Graduate School of Natural Science and Technology
Division of Natural System, Graduate School of Natural Science and Technology
Course in Biosystems, School of Biological Science and Technology, College of Science and Engineering

Laboratory

　TEL:076-264-6229　FAX:076-264-6230

Academic Background

【Academic background(Doctoral/Master's Degree)】
Kyoto University　Doctor　Graduate School, Division of Natural Science　1994　Completed
【Academic background(Bachelor's Degree)】
Kyushu University　1988

Career

HSP Research Institute(1994/04/01-2000/03/17)

Year & Month of Birth

1965/09

Academic Society

Award

○grant from Yamada Science Foundation(2001/09/25)

Specialities

Functional biochemistry

Speciality Keywords

stress response, molecular chaperone, ATP-dependent protease

Research Themes

Substrate recognition mechanism of Hsp70 chaperone system

It has been thought that a polypeptide spontaneously folds into its functional state according to the primary amino acid sequence. However, most newly synthesized polypeptides require a set of molecular chaperones to fold their native conformations. Because cytosol is highly crowded with macromolecules, a newly synthesized polypeptide could improperly interact with them and fail to fold. Molecular chaperones protects newly synthesized polypeptides from improper interactions or aggregation, and assist them in maturing into proteins. Although Hsp70 is the major chaperone and has been studied for long time, its substrate-recognition mechanism is not perfectly understood. I have studied Hsp70 using techniques of molecular biology and biochemistry.

Books

Papers

　Two J domains ensure high cochaperone activity of DnaJ, Escherichia coli heat shock protein 40　Tomoya Uchida, Masaaki Kanemori　Journal of Biochemistry　164　2　153-163　2018/07/11
　DnaJ-Promoted Binding of DnaK to Multiple Sites on σ32 in the Presence of ATP　Aki Noguchi, Ayami Ikeda, Moeka Mezaki, Yoshihiro Fukumori, Masaaki Kanemori　JOURNAL OF BACTERIOLOGY　196　9　1694-1703　2014/05
　Synergistic binding of DnaJ and DnaK chaperones to the heat shock transcriptionfactor s32 assures its characteristic high metabolic instability. Implications for heat shock protein 70 (Hsp70)-Hsp40 mode of function.　Hirotaka Suzuki, Ayami Ikeda, Sachie Tsuchimoto, Ko-ichi Adachi, Aki Noguchi, Yoshihiro Fukumori, Masaaki Kanemori　JOURNAL OF BIOLOGICAL CHEMISTRY　287　23　19275-19283　2012/06
　Conserved region 2.1 of Escherichia coli heat shock transcription factor s32 is required for modulating both metabolic stability and transcriptional activity　JOURNAL OF BACTERIOLOGY　186　22　7474-7480　2004/11
　Dynamic interplay between antagonistic pathways controlling the s32 level in Escherichia coli.　Proceedings of the National Academy of Sciences of the United States of America　97　11　5860-5865　2000/05

show all

　Marked instability of the s32 heat shock transcription factor at high temperature - implication for heat shock regulation.　The Journal of Biological Chemistry　273　31　22002-22007　1999/07
　Overexpression of trigger factor prevents aggregation of recombinant proteins in Escherichia coli.　Applied and Emvironmental Microbiology　66　3　884-889　2000/03
　The ATP-dependent HslVU/ClpQY protease participates in turnover of cell division inhibitor SulA in Escherichia coli.　JOURNAL OF BACTERIOLOGY　181　12　3674-3680　1999/06
　Heat-induced synthesis of s32 in Escherichia coli: structural and functional dissection of rpoH mRNA secondary structure.　JOURNAL OF BACTERIOLOGY　181　2　401-410　1999/01
　Chaperone coexpression plasmids: differential and synergistic roles of DnaK-DnaJ-GrpE and GroEL-GroES in assisting folding of an allergen of Japanese cedar pollen, Cryj2, in Escherichia coli.　Applied and Emvironmental Microbiology　64　5　1694-1699　1998/05
　Nitrate reductase from the magnetotactic bacterium Magnetospirillum magnetotacticum MS-1: purification and sequence analyses.　CANADIAN JOURNAL OF MICROBIOLOGY　49　3　197-206　2003/03
　Synergistic roles of HslVU and other ATP-dependent proteases in controlling in vivo turnover of s32 and abnormal proteins in Escherichia coli.　JOURNAL OF BACTERIOLOGY　179　23　7219-7225　1997/12
　Purification, characterization and sequence analyses of extracellular giant hemoglobin from Oligobrachia mashikoi　Taro Nakagawa, Seiko Onoda, Masaaki Kanemori, Yuichi Sasayama, Yoshinori Fukumori　ZOOLOGICAL SCIENCE　22　3　283-291　2005/03
　Identification of endosymbionts in Oligobrachia mashikoi (Siboglinidae, Annelida)　Norihiro Kubota, Masaaki Kanemori, Yuichi Sasayama, Masato Aida, Yoshihiro Fukumori　Microbes and Environments　22　2　136-144　2007/05
　Actual distribution of bacteriocytes in the trophosome of a beard worm (Oligobrachia mashikoi, Siboglinidae, Annelida): clarification using whole-mount in situ hybridization　M. Deguchi, N. Kubota, A. Matsuno, M. Kanemori, Y. Fukumori, Y. Sasayama　ACTA ZOOLOGICA　88　2　129-135　2007/04
　Direct evidence that extra-cellular giant hemoglobin is produced in chloragogen tissues in a beard worm, Oligobrachia mashikoi (Frenulata, Siboglinidae, Annelida)　S. Nakahama, T. Nakagawa, M. Kanemori, Y. Fukumori, and Y. Sasayama　Zoological Science　25　1247-1252　2008
　Distribution and population of free-living cells related to endosymbiont A harbored in Oligobrachia mashikoi (a Siboglinid Polychaete)　M. Aida, M. Kanemori, N. Kubota, M. Matada, Y. Sasayama, and Y. Fukumori　Microbes and Environments　23　1　81-88　2008
　GroEL-substrate-GroES ternary complexes are an important transient intermediate of the chaperonin cycle.　JOURNAL OF BIOLOGICAL CHEMISTRY　277　52　50621-50628　2002/12

Conference Presentations

Others

　The heat shock response: Regulation and function.　3-18　2000

Arts and Fieldwork

Patent

Theme to the desired joint research

Grant-in-Aid for Scientific Research

○「分子シャぺロンやＡＴＰ依存性プロテアーゼにより認識されるσ32上の構造の同定」(2001-)　

Associate Professor　KANEMORI Masaaki

Faculty, Affiliation

College and School Educational Field

Laboratory

Academic Background

Career

Year & Month of Birth

Academic Society

Award

Specialities

Speciality Keywords

Research Themes

Substrate recognition mechanism of Hsp70 chaperone system

Books

Papers

Conference Presentations

Others

Arts and Fieldwork

Patent

Theme to the desired joint research

Grant-in-Aid for Scientific Research

Competitive research funding,Contribution

Collaborative research,Consignment study

Classes (Bachelors)

Classes (Graduate Schools)

International Project

International Students

Lecture themes

Others (Social Activities)

Associate Professor KANEMORI Masaaki

Faculty, Affiliation

College and School Educational Field

Laboratory

Academic Background

Career

Year & Month of Birth

Academic Society

Award

Specialities

Speciality Keywords

Research Themes

Substrate recognition mechanism of Hsp70 chaperone system

Books

Papers

Conference Presentations

Others

Arts and Fieldwork

Patent

Theme to the desired joint research

Grant-in-Aid for Scientific Research

Competitive research funding,Contribution

Collaborative research,Consignment study

Classes (Bachelors)

Classes (Graduate Schools)

International Project

International Students

Lecture themes

Others (Social Activities)

Associate Professor　KANEMORI Masaaki