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Researcher Information

last modified：2024/12/03

Professor　ANDO Toshio

Mail Laboratory Website

Faculty, Affiliation

WPI Nano Life Science Institute
Nano Life Science institute (WPI-NanoLSI) Principal Investigator
Head of Imaging Research Division, Bio-AFM Frontier Research Center

College and School Educational Field

Division of Mathematical and Physical Science, Graduate School of Natural Science and Technology
Division of Mathematical and Physical Sciences, Graduate School of Natural Science and Technology

Laboratory

Biophysics Lab.　TEL:076-264-5663　FAX:076-264-5739

Academic Background

【Academic background(Doctoral/Master's Degree)】
Waseda University　Doctor　Graduate School, Division of Science and Engineering　Physics and Applied Physics　198003　Completed
【Academic background(Bachelor's Degree)】
Waseda University　197403
【Degree】
Doctor of Science

Career

Kanazawa University　Natural Science & Technology(2003/04/01-)
Kanazawa University　Faculty of Science(1996/07/01-2003/03/31)
Kanazawa University　Faculty of Science(1992/08/01-1996/06/30)
Kanazawa University　Faculty of Science(1986/04/01-1992/07/31)
University of California, San Francisco　Cardiovascular Research Institute　Assistant Research Biophysicist(1983/01/01-1986/03/31)
UC San Francisco, CVRI, Posdoc(1980/04/01-1982/12/31)

Year & Month of Birth

1951/03

Academic Society


American Physical Society

Jananese Socisty of Applied Physics
The Surface Science Society of Japan
Japanese Society of Microscopy

Award

○Medal with Purple Ribbon(2023/04)
○Toray Science and Technology Prize(2023/03)
○Toray Science and Technology Prize(2023/03)
○Toray Science and Technology Prize(2023/03)
○Harushie Inoue Prize(2016/07)
○Shimadzu Prize(2014/02)
○Doctor Honoris Causa(2014/11)
○Chairman's Prize of the Japanese Association of Invention(2013/06)
○Science & Technology Award(2013/04)
○Distinguished Contribution Award for Research and Education(2013/11)
○Kanazawa City Culture Prize(2012/11/03)
○University of Pennsylvania NBIC Research Excellence Award in Nanotechnology (2012/10/24)
○Award of the Japanese Society of Microscopy (2011/05/17)
○Yamazaki-Teiichi Prize(2010/09)
○Award of the Surface Science Society of Japan (2010/05/22)
○Sakaki Prize (JSPS)(2008/09/16)
○Best Paper Award of the Japan Society of Applied Physics(2007/09/07)
○2007 Distinguished Service Award of the President of Science Council of Japan(2007/06/16)
○2007 Distinguished Service Award of the President of Science Council of Japan(2007/06/16)
○Nanoprobe Technology Prize(2004/07/21)
○Nikkei BP Technology Prize(2003/04/04)

Specialities

Biophysics、Nanomaterials chemistry

Speciality Keywords

Scanning Probe Microscopy,Bio-imaging,High-speed atomic force microscopy,High-speed AFM,atomic force microscopy,motor proteins,Atomic Force Microscope,single-molecule analysis

Research Themes

Study on Motor Proteins

Study on Motor Proteins Motor proteins cordinate cell motility. We study them to understand their functional mechanism. For this purpose, we have been developing various techniques including high-speed AFM and fluorescence microscopy techniques. The left movie of walking myosin V comes from our paper published in Nature (Article) on November 4, 2010. Please look at other movies at our lab website.

Development and Biological Application of High-speed Atomic Force Microscopy

Development and Biological Application of High-speed Atomic Force Microscopy We have developed high-speed AFM capable of simultaneously recording the structure and dynamics of biological molecules at high spatiotemporal resolution. The capacity has now nearly reached the theoretical upper limit. Using this state-of-the-art microscopy, we are now imaging various functioning biomolecules in action to understand their functional mechanisms. The left images show dynamic structural changes of bacteriorhodopsin in response to light illumination (a, under dark; b, under light illumination).

Awards

Awards We have been awarded honorable prizes for the achievement of developing high-speed atomic force microscopy and its biological applications. This is the result of efforts by colleagues and many students in my lab.
2014.11.18 Doctor Honoris Causa from the Aix-Marseille Univ.
2014.2.17 Shimadzu Prize (Shimadzu Foundation for Promotion of Science & Technology)
2013.11.2 Distinguished Contribution Award for Research and Education, Kanazawa Univ.
2013.6.18 Chairman's prize of the Japanese Association of Invention
2013.4.16 Science & Technology Award of the Minister of Education, Culture, Sports, Science and Technology
2012.11.3 Kanazawa City Culture Prize
2012.10.24 Univ. of Pennsylvania NBIC Award for Research Excellence in Nanotechnology
2011.5.17 Best Paper Award of the Japanese Society of Microscopy
2010.9.16 Yamazaki-Teiichi Prize (Foundation for Promotion of Material Science and Technology of Japan)
2010.5.22 Award of the Surface Science Society of Japan
2008.9.16 Sakaki Prize (JSPS)
2007.9.4 Best Paper Award of the Japan Society of Applied Physics
2007.6.16 Distinguished Service Award of the President of Science Council of Japan
2005.11.3 Hokkoku Culture Prize
2004.7.21 Nanoprobe Technology Prize (JSPS)
2003.4.4 Nikkei BP Technology Prize

　 　

Books

  • 　Toshio Ando　High-speed atomic force microscopy in biology.　Springer, Berlin, Heiderberg　2022/02
  • 　Takayuki Uchihashi, Noriyuki Kodera, Toshio Ando 　Noncontact Atomic Force Microscopy Vol.3 (Seizo Moprita, Franz J. Giessibl, Ernst Meyer, Roland Wiesendanger, Eds)　Springer　2015
  • 　Takayuki Uchihahsi, Noriyuki Kodera, Toshio Ando　Atomic Force Microscopy in Nanobiology (Kunio Takeyasu Ed.) 　Pan Stanford Publishing　2014
  • 　Toshio Ando, Takayuki Uchihashi 　Nanoscale Liquid Interfaces: Wetting, Patterning, and Force Microscopy at the Molecular Scale (Thierry ONDARÇUHU, Jean-Pierre Aimé Ed.)　Pan Stanford Publishing　2013
  • 　Takayuki Uchihashi, Noriyuki Kodera, Toshio Ando　Single-molecule Studies of Proteins. Biophysics for the Life Sciences Vol 2 (Andres Oberhauser, ed) 　Springer　2013

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  • 　Noriyuki Kodera, Toshio Ando　Springer series Methods in Molecular Biology, vol. 897, part 1 "Experimental Tools for the Intrinsically Disordered Protein Analysis　Springer　2012/01
  • 　Techniques for developing high-speed AFM　Springer　2011/07
  • 　T. Ando　Single Molecule Dynamics in Life Science　Wiley-VCH　2008/08
  • 　T. Ando, T. Uchihasi　High-speed atomic force microscopy　Springer-Verlarg 　2009/09
  • 　T. Ando　Roadmap of Scanning Probe Microscopy　Springer-Verlag 　2006/06
  • 　T. Uchihashi, T. Ando　High-speed atomic force microscopy for dynamic biological imaging　Pan Stanford Publishing Pte. Ltd.　2011/07
  • 　Single-molecule Biology (Yoshie Harada & Shin'ichi Ishiwata Ed)　2014/09

Papers

  • 　Structural and dynamics analysis of intrinsically disordered proteins by high-speed atomic force microscopy　Noriyuki Kodera,Daisuke Noshiro,Sujit K. Dora,Tetsuya Mori,Johnny Habchi,David Blocquel,Antoine Gruet,Marion Dosnon,Edoardo Salladini,Christophe Bignon,Yuko Fujioka,Takashi Oda,Nobuo N. Noda,Mamoru Sato,Marina Lotti,Mineyuki Mizuguchi,Sonia Longhi,Toshio Ando　Nature Nanotechnology　16　2　181　2021/02　
  • 　Geometrical Characterization of Glass Nanopipettes with Sub-10 nm Pore Diameter by Transmission Electron Microscopy　Kazuki Shigyou,Linhao Sun,Riku Yajima,Shohei Takigaura,Masashi Tajima,Hirotoshi Furusho,Yousuke Kikuchi,Keisuke Miyazawa,Takeshi Fukuma,Azuma Taoka,Toshio Ando,Shinji Watanabe　Analytical Chemistry　92　23　15388　2020/12/01　
  • 　High-Speed Atomic Force Microscopy Reveals Factors Affecting the Processivity of Chitinases during Interfacial Enzymatic Hydrolysis of Crystalline Chitin　Mingbo Qu,Takahiro Watanabe-Nakayama,Shaopeng Sun,Kenichi Umeda,Xiaoxi Guo,Yuansheng Liu,Toshio Ando,Qing Yang　ACS Catalysis　10　22　13606　2020/11/20　
  • 　Two-State Exchange Dynamics in Membrane-Embedded Oligosaccharyltransferase Observed in Real-Time by High-Speed AFM　Yuki Kawasaki,Hirotaka Ariyama,Hajime Motomura,Daisuke Fujinami,Daisuke Noshiro,Toshio Ando,Daisuke Kohda　Journal of Molecular Biology　432　22　5951　2020/11
  • 　Spatiotemporally tracking of nano-biofilaments inside the nuclear pore complex core　Mahmoud Shaaban Mohamed,Masaharu Hazawa,Akiko Kobayashi,Laurent Guillaud,Takahiro Watanabe-Nakayama,Mizuho Nakayama,Hanbo Wang,Noriyuki Kodera,Masanobu Oshima,Toshio Ando,Richard W. Wong　Biomaterials　256　120198　2020/10

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  • 　High-Speed AFM Reveals Molecular Dynamics of Human Influenza A Hemagglutinin and Its Interaction with Exosomes　Keesiang Lim,Noriyuki Kodera,Hanbo Wang,Mahmoud Shaaban Mohamed,Masaharu Hazawa,Akiko Kobayashi,Takeshi Yoshida,Rikinari Hanayama,Seiji Yano,Toshio Ando,Richard W. Wong　Nano Letters　20　9　6320　2020/09/09　
  • 　Self- and Cross-Seeding on α-Synuclein Fibril Growth Kinetics and Structure Observed by High-Speed Atomic Force Microscopy.　Takahiro Watanabe-Nakayama,Maika Nawa,Hiroki Konno,Noriyuki Kodera,Toshio Ando,David B Teplow,Kenjiro Ono　ACS nano　14　8　9979　2020/08/25
  • 　Studies on the impellers generating force in muscle　Toshio Ando　Biophysical Reviews　12　4　767　2020/08　
  • 　Diversity of physical properties of bacterial extracellular membrane vesicles revealed through atomic force microscopy phase imaging.　Yousuke Kikuchi,Nozomu Obana,Masanori Toyofuku,Noriyuki Kodera,Takamitsu Soma,Toshio Ando,Yoshihiro Fukumori,Nobuhiko Nomura,Azuma Taoka　Nanoscale　12　14　7950　2020/04/14
  • 　Millisecond Conformational Dynamics of Skeletal Myosin II Power Stroke Studied by High-Speed Atomic Force Microscopy　Oleg S. Matusovsky,Noriyuki Kodera,Caitlin MacEachen,Toshio Ando,Yu-Shu Cheng,Dilson E. Rassier　ACS Nano　15　2　2229　2021/02/23　
  • 　Capturing transient antibody conformations with DNA origami epitopes　Ping Zhang,Xiaoguo Liu,Pi Liu,Fei Wang,Hirotaka Ariyama,Toshio Ando,Jianping Lin,Lihua Wang,Jun Hu,Bin Li,Chunhai Fan　Nature Communications　11　1　2020/12　
  • 　Biophysics in Kanazawa University　Toshio Ando　Biophysical Reviews　2020/02
  • 　High-speed near-field fluorescence microscopy combined with high-speed atomic force microscopy for biological studies.　Takayuki Umakoshi,Shingo Fukuda,Ryota Iino,Takayuki Uchihashi,Toshio Ando　Biochimica et biophysica acta. General subjects　1864　2　129325-1　2020/02
  • 　Phase separation organizes the site of autophagosome formation　Yuko Fujioka,Jahangir Md. Alam,Daisuke Noshiro,Kazunari Mouri,Toshio Ando,Yasushi Okada,Alexander I. May,Roland L. Knorr,Kuninori Suzuki,Yoshinori Ohsumi,Nobuo N. Noda　Nature　578　7794　301　2020/02　
  • 　Development of high-speed ion conductance microscopy　Shinji Watanabe,Satoko Kitazawa,Linhao Sun,Noriyuki Kodera,Toshio Ando　Review of Scientific Instruments　90　12　123704　2019/12/01　
  • 　Structure of the mitochondrial import gate reveals distinct preprotein paths　Y. Araiso,A. Tsutsumi,J. Qiu,K. Imai,T. Shiota,J. Song,C. Lindau,L.-S. Wenz,H. Sakaue,K. Yunoki,S. Kawano,J. Suzuki,M. Wischnewski,C. Schütze,H. Ariyama,T. Ando,T. Becker,T. Lithgow,N. Wiedemann,N. Pfanner,M. Kikkawa,T. Endo　Nature　575　395　2019/10
  • 　Thermally driven approach to fill sub-10-nm pipettes with batch production　Linhao Sun,Kazuki Shigyou,Toshio Ando,Shinji Watanabe　Anal. Chem.　91　14080　2019/10
  • 　Gliding machinery of Mycoplasma mobile visualized by High-speed AFM　Kohei Kobayashi,Noriyuki Kodera,Yuhei Tahara,Takuma Toyonaga,Taishi Kasai,Toshio Ando,Makoto Miyata　Microscopy　54　2　67　2019/08　
  • 　High-speed atomic force microscopy　T. Ando　Curr. Opin. Chem. Biol. 51, 105-112　51　105　2019/08
  • 　High-speed atomic force microscopy and its future prospects　Toshio Ando　Biophysical Reviews　10　2　285　2018/04/01
  • 　Structural insights into the substrate specificity switch mechanism of the type III protein export apparatus　Y. Inoue,Y. Ogawa,M. Kinoshita,N. Terahara,M. Shimada,N. Kodera,T. Ando,K. Namba,A. Kitao,K. Imada,T. Minamino　Structure　27　6　965　2019/06
  • 　Inner lumen proteins stabilize doublet microtubules in cilia and flagella.　Mikito Owa,Takayuki Uchihashi,Haru-Aki Yanagisawa,Takashi Yamano,Hiro Iguchi,Hideya Fukuzawa,Ken-Ichi Wakabayashi,Toshio Ando,Masahide Kikkawa　Nature communications　10　1　1143　2019/03/08
  • 　Structure of the UHRF1 Tandem Tudor Domain bound to a methylated non-histone protein, LIG1, reveals rules for binding and regulation　S. Kori,L. Ferry,S. Matano,T. Jimenji,N. Kodera,T. Tsusaka,R. Matsumura,T. Oda,M. Sato,N. Dohmae N,T. Ando,Y. Shinkai,P. A. Defossez,K. Arita　Structure　27　3　485　2019/03
  • 　Cationic polymethacrylate-copolymer acts as an agonist for β-amyloid and antagonist for amylin fibrillation　B. R. Sahoo,T. Genjo,T. Watanabe-Nakayama,A. K. Stoddard,T. Ando,K. Yasuhara,C. A. Fierke,A. Ramamoorthy　Chem. Sci.　10　3976　2019/02
  • 　The clustering-induction of RANKL molecules could stimulate early osteroblast differentiation　E. Sone,D. Noshiro,Y. Ikebuchi,M. Nakagawa,M. Khan,Y. Tamura,M. Ikeda,M. Oki,R. Murali,T. Fujimori,T. Yoda,M. Honma,H. Suzuki,T. Ando,K. Aoki　Biophys. Biochem. Res. Commun.　509　2　435　2019/02
  • 　Metastable asymmetrical structure of a shaftless V1 motor　Shintaro Maruyama,Kano Suzuki,Motonori Imamura,Hikaru Sasaki,Hideyuki Matsunami,Kenji Mizutani,Yasuko Saito,Fabiana L. Imai,Yoshiko Ishizuka-Katsura,Tomomi Kimura-Someya,Mikako Shirouzu,Takayuki Uchihashi,Toshio Ando,Ichiro Yamato,Takeshi Murata　Science Advances　5　1　eaau8149　2019/01　
  • 　Single-unit imaging of membrane protein-embedded nanodiscs from two oriented sides by high-speed atomic force microscopy　T. Haruyama,Y. Sugano,N. Kodera,T. Uchihashi,T. Ando,Y. Tanaka,H. Konno,T. Tsukazaki　Structure　27　152　2019/01
  • 　Free energy landscape and dynamics of supercoiled DNA by high-speed tomic force microscopy　T. Brouns,H. De. Keersmaecker,S. Konrad,N. Kodera,T. Ando,J. Lipfert,S. De Feyter,W. Vanderlinden　ACS Nano　12　12　11907　2018/10
  • 　Revealing circadian mechanisms of integration and resilience by visualizing clock proteins working in real time　T. Mori,S. Sugiyama,M. Byme,C. H. Johnson,T. Uchihashi,T. Ando　Nature Communications　9　3245-1　2018/08
  • 　Topical Review: The 2018 correlative microscopy techniques roadmap　T. Ando,authors　J. Phys. D: Appl. Phys.　51　44　443001-1　2018/08
  • 　Quantum-dot antibody conjugation visualized at the single-molecule scale with high-speed atomic force microscopy　Takayuki Umakoshi,Hikari Udaka,Takayuki Uchihashi,Toshio Ando,Miho Suzuki,Takeshi Fukuda　Colloids and Surfaces B: Biointerfaces　167　267　2018/07/01
  • 　Dynamic structural states of CIpB involved in its disaggregation function　Takayuki Uchihashi,Yo-hei Watanabe,Yosuke Nakazaki,Takashi Yamasaki,Hiroki Watanabe,Takahiro Maruno,Kentaro Ishii,Susumu Uchiyama,Chihong Song,Kazuyoshi Murata,Ryota Iino,Toshio Ando　NATURE COMMUNICATIONS　9　2147-1　2018/06
  • 　Substrate protein dependence of GroEL-GroES interaction cycle revealed by high-speed AFM imaging　D. Noshiro,T. Ando　Philosophical Transactions Royal Society B　373　1749　20170180-1　2018/05
  • 　Insight into structural remodeling of the FlhA ring responsible for bacterial flagellar type III protein export　Naoya Terahara,Yumi Inoue,Noriyuki Kodera,Yusuke V. Morimoto,Takayuki Uchihashi,Katsumi Imada,Toshio Ando,Keiichi Namba,Tohru Minamino　Science Advances　4　4　eaao7054-1　2018/04/25
  • 　Chaperonin GroEL-GroES functions as both alternating and non-alternating engines　D. Yamamoto and T. Ando　J. Mol. Biol.　428　3090-3101 　2016
  • 　High-speed atomic force microscopy reveals strongly polarized movement of clostridial collagenase along collagen fibrils　T. Watanabe-Nakayama, M. Itami, N. Kodera, T. Ando and H. Konno　Scientific Reports　6　28975　2016
  • 　The Intrinsically Disordered Protein Atg13 Mediates Supramolecular Assembly of Autophagy Initiation Complexes　Hayashi Yamamoto, Yuko Fujioka, Sho W. Suzuki, Daisuke Noshiro, Hironori Suzuki, Chika Kondo-Kakuta, Yayoi Kimura, Hisashi Hirano, Toshio Ando, Nobuo N. Noda, Yoshinori Ohsumi　Dev. Cell 　38　86-99　2016
  • 　Functional extension of high-speed atomic force microscopy　T. Uchihashi, H. Watanabe, S. Fukuda, M. Shibata and T. Ando　Ultramicroscopy　160　182-196 　2016
  • 　Two-ball structure of the flagellar hook-length control protein FliK as revealed by high-speed atomic force microscopy　N. Kodera, K. Uchida, T. Ando, and S. Aizawa　J. Mol. Biol. 　427　406-414 　2015
  • 　Method of mechanical holding of cantilever chip for tip-scan high-speed atomic force microscopy　Shingo Fukuda, Takayuki Uchihashi, Toshio Ando　Rev. Sci. Instrum. 　86　063703　2015
  • 　Cofilin-induced unidirectional cooperative conformational changes in actin filaments revealed by high-speed AFM　Kien Xuan Ngo, Noriyuki Kodera, Eisaku Katayama, Toshio Ando and Taro Q.P. Uyeda　e-Life 　4　e04806　2015
  • 　Potential pre-pore trimer formation by the Bacillus thuringiensis mosquito-specific toxin: Molecular insights into a critical prerequisite of membrane-bound monomers　Wilaiwan Sriwimol, Aratee Aroonkesorn, Somsri Sakdee, Chalermpol Kanchanawarin, Takayuki Uchihashi, Toshio Ando and Chanan Angsuthanasombat 　J. Biol. Chem.　290　34　20793-20803 　2015
  • 　CYK4 Promotes Antiparallel Microtubule Bundling by Optimizing MKLP1 Neck Conformation　T. Davies, N. Kodera, G. S. Kaminski Schierle, E. Rees, M. Erdelyi, C. F. Kaminski, T. Ando, M. Mishima 　PloS Biol.　13　4　e1002121 (26 pp)　2015
  • 　Long-tip high-speed atomic force microscopy for nanometer-scale imaging in live cells　M. Shibata, T. Uchihashi, T. Ando and R. Yasuda　Scientific Reports 　5　8724 (7 pp)　2015
  • 　Probing structural dynamics of an artificial protein cage using high-speed atomic force microscopy　M. Imamura, T. Uchihashi, T. Ando, A. Leifert, U. Simon, A. D. Malay and J. G. Heddle　Nano Lett. 　15　2　1331-1335　2015
  • 　IgGs are made for walking on bacterial and viral surfaces　J. Preiner, N. Kodera, J. Tang, A. Ebner, M. Brameshuber, D. Blaas, N. Gelbmann, H. Gruber, T. Ando, and P. Hinterdorfer　Nature Communications 　5　4394 (8 pp)　2014
  • 　Multiple Interactions of the Intrinsically Disordered Region between the N-terminal Helicase and C-terminal Nuclease Domains of the Archaeal Hef Protein 　S. Ishino, T. Yamagami, M. Kitamura, N. Kodera, T. Mori, S. Sugiyama, T. Ando, N. Goda, T. Tenno, H. Hiroaki, and Y. Ishino　J. Biol. Chem.　289　21627-21639 　2014
  • 　High-speed AFM imaging　Toshio Ando　Curr. Opin. Struct. Biol. 　28　63-68　2014
  • 　The path to visualization of walking myosin V by high-speed atomic force microscopy　N. Kodera and T. Ando　Biophys. Rev.　6　237-260 　2014
  • 　Single-molecule imaging analysis of elementary reaction steps of Trichoderma Reesei cellobiohydrolase I (Cel7A) hydrolyzing crystalline cell　Y. Shibafuji, A. Nakamura, T. Uchihashi, N. Sugimoto, S. Fukuda, H. Watanabe, M. Samejima, T. Ando, H. Noji, A. Koivula, K. Igarashi, and R. Iino　J. Biol. Chem.　289　14056-14065 　2014
  • 　Filming biomolecular processes by high-speed atomic force microscopy　T. Ando, T. Uchihashi, and S. Scheuring　Chem. Rev.　114　6　3120-3188 　2014
  • 　Trade-off between processivity and hydrolytic velocity of cellobiohydrolases at the surface of crystalline cellulose　A. Nakamura, H. Watanabe, T. Ishida, T. Uchihashi, M. Wada, T. Ando, K. Igarashi, and M. Samejima　J. Am. Chem. Soc. 　136　4584-4592　2014
  • 　A dynamic PID controller for high-speed atomic force microscopy　N. Kodera, M. Sakashita, and T. Ando　REVIEW OF SCIENTIFIC INSTRUMENTS　77　8　083704-7　2006
  • 　Two-way traffic of glycoside hydrolase family 18 processive chitinases on crystalline chitin　K. Igarashi, T. Uchihashi, T. Uchiyama, H. Sugimoto, M. Wada, K. Suzuki, S. Sakuda, T. Ando, T. Watanabe, and M. Samejima　Nature Communications　5　3975　2014
  • 　Identification of the specific IQ motif of myosin V from which calmodulin dissociates in the presence of Ca2+.　H. Koide, T. Kinoshita, Y. Tanaka, S. Tanaka, N. Nagura, G. Meyer zu Hoerste, A. Miyagi　BIOCHEMISTRY　45　38　11598-11604　2006
  • 　High-speed atomic force microscopic observation of ATP-dependent rotation of the AAA+ chaperone p97　Kentaro Noi, Daisuke Yamamoto, Shingo Nishikori, Ken-ichi Arita-Morioka, Toshio Ando and Teru Ogura　Structure　21　1992-2002　2013
  • 　Fast phase imaging in liquids using a rapid scan atomic force microscope　T. Uchihashi, H. Yamashita, T. Ando　APPLIED PHYSICS LETTERS　89　21　213112-3　2006
  • 　High-speed atomic force microscopy of protein dynamics: myosin on actin and rotary enzyme F1-ATPase　Toshio Ando　Microscopy & Analysis 　27　3　10-13　2013
  • 　Feed-forward compensation for high-speed atomic force microscopy imaging of biomolecules.　T. Uchihashi, N. KOdera, H. Ito, H. Yamashita, T. Ando　JAPANESE JOURNAL OF APPLIED PHYSICS PART 1-REGULAR PAPERS SHORT NOTES & REV　45　3B　1904-1908　2006
  • 　Real-Time Visualization of Assembling of a Sphingomyelin-Specific Toxin　Neval Yilmaz, Taro Yamada, Peter Greimel, Takayuki Uchihashi, Toshio Ando, and Toshihide Kobayashi　Biophys. J. 　105　1397-1405　2013
  • 　Fast-scaaning atomic foece microscopy reveals the ATP/ADP-dependent conformational changes of GroEL.　M. Yokokawa, S.H. Yoshimura, Y. Naito, T. Ando, A. Yagi, S. Sakai, K. Takeyasu　EMBO JOURNAL　25　x　4567-4576　2006
  • 　High-speed atomic force microscopy for studying dynamic behavior of protein molecules at work.　T. Ando, T. Uchihashi, N. Kodera, A. Miyagi, R. Nakakita, H. Yamashita, M. Sakashita　JAPANESE JOURNAL OF APPLIED PHYSICS PART 1-REGULAR PAPERS SHORT NOTES & REV　45　3B　1897-1903　2006
  • 　High-speed AFM and applications to biomolecular systems　T. Ando, T. Uchihashi, and N. Kodera　Annual Review of Biophysics　42　393-414 　2013
  • 　Role of trimer-trimer interaction of bacteriorhodopsin studied by optical spectroscopy and high-speed atomic force microscopy　H. Yamashita, K. Inoue, M. Shibata, T. Uchihashi, J. Sasaki, H. Kandori, and T. Ando　J. Struct. Biol. 　184　2-11　2013
  • 　Fast-scanning atomic force microscopy reveals the molecular mechanism of DNA cleavage by ApaI endonuclease.　M. Yokokawa, S.H. Yoshimura, Y. Naito, T. Ando, A. Yagi, N. Sakai, K. Takeyasu　IEE Proc. Nanobiotechnology　153　4　60-66　2006
  • 　Active damping of the scanner for high-speed atomic force microscopy.　REVIEW OF SCIENTIFIC INSTRUMENTS　76　5　053708　2005
  • 　Wide-area scanner for high-speed atomic force microscopy　H. Watanabe, T. Uchihashi, T. Kobashi, M. Shibata, J. Nishiyama, R. Yasuda, and T. Ando　Review of Scientific Instruments　84　5　053702　2013
  • 　High-speed AFM for studying the dynamic behavior of protein molecules at work　T. Ando, T. Uchihashi, N. Kodera, A. Miyagi, R. Nakakita, H. Yamashita, K. Matada　e-J. Surf. Sci. Nanotechnol. 　3　384-392　2005
  • 　Molecular Machines directly observed by high-speed atomic force microscopy　FEBS LETTERS　587　8　997-1007　2013
  • 　High-speed atomic force microscopy　Microscopy　62　1　81-93 　2013
  • 　Polarized Actin Bundles Formed by Human fascin-1: Their Sliding and Disassembly on Myosin II and Myosin V in vitro.　R. Ishikawa, T. Sakamoto, T. Ando　JOURNAL OF NEUROCHEMISTRY　87　676-685　2003
  • 　Phosphorylation-Coupled Intramolecular Dynamics of Unstructured Regions in Chromatin Remodeler FACT　M. Hashimoto, N. Kodera, Y. Tsunaka, M. Oda, M. Tanimoto, T. Ando, K. Morikawa, and S. Tate　BIOPHYSICAL JOURNAL　104　10　2222-2234 　2013
  • 　High-resolution Imaging of Myosin Motor in Action by a High-speed Atomic Force Microscope.　N. Kodera, T. Ando　ADVANCES IN PROTEIN CHEMISTRY　538　119-127　2003
  • 　A high-speed Atomic force microscope for studying biological macromolecules in action.　CHEMPHYSCHEM　4　1196-1202　2003
  • 　High-speed atomic force microscope combined with single-molecule fluorescence microscope　S. Fukuda, T. Uchihashi, R. Iino, Y. Okazaki, M. Yoshida, K. Igarashi　REVIEW OF SCIENTIFIC INSTRUMENTS　84　7　073706 　2013
  • 　Guide to video recording of structure dynamics and dynamic processes of proteins by high-speed atomic force microscopy　T. Uchihashi, N. Kodera, and T. Ando　Nature Protocols　7　6　1193-1206 　2012
  • 　A high-speed atomic force microscope for studying biological macromolecules in action　JAPANESE JOURNAL OF APPLIED PHYSICS PART 1-REGULAR PAPERS SHORT NOTES & REV　41　7B　4851-4856　2002
  • 　Single molecule imaging on living bacterial cell surface by high-speed AFM　H. Yamashita, A. Taoka, T. Uchihashi, T. Asano, T. Ando, and Y. Fukumori　JOURNAL OF MOLECULAR BIOLOGY　422　2　300-309　2012
  • 　A high-speed atomic force microscope for studying biological macromolecules　PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AME　98　22　12468-12472　2001
  • 　High-speed atomic force microscopy　T. Ando, T. Uchihashi, and N. Kodera　JAPANESE JOURNAL OF APPLIED PHYSICS　51　08KA02　2012
  • 　Link between the enzymatic and mechanical behavior in an actomyosin motor　I. Amitani, T. Sakamoto, T. Ando　Biophys. J.　80　379-397 　2001
  • 　High-speed atomic force microscopy coming of age　NANOTECHNOLOGY　23　6　062001　2012
  • 　Visualization of cellobiohydrolase I from Trichoderma reesei moving on crystalline cellulose using high-speed atomic force microscopy　K. Igarashi, T. Uchihashi, A. Koivula, M. Wada, S. Kimura, M. Penttilä, T. Ando, and M. Samejima　METHODS IN ENZYMOLOGY　510　169-182 　2012
  • 　Direct observation of processive movement by individual myosin V molecules　Biochem. Biophys. Res. Commun.　272　586-590　2000
  • 　High-speed atomic force microscopy reveals rotary catalysis of rotorless F1-ATPase　T. Uchihashi, R. Iino, T. Ando, and H. Noji　SCIENCE　333　6043　755-758　2011
  • 　Single-fluorophore imaging with an unmodified epifluorescence microscope and conventional video camera　J. Microscopy　195　178-182　1999
  • 　Dynamics of nucleosomes assessed with time-lapse high speed atomic force microscopy　A. Miyagi, T. Ando and Y. L. Lyubchenko　Biochemistry　50　7901-7908 　2011
  • 　Scanning force microscopy of the interaction events between a single molecule of heavy meromyosin and actin　Biochem. Biophys. Res. Commun.　234　178-182　1997
  • 　Self-assembly properties and dynamic of synthetic proteo-nucleic building blocks in solution and on surfaces　 A. Laisne, M. Ewald, T. Ando, E. Lesniewska, D. Pompon　Bioconjugate Chem. 　22　1824-1834 　2011
  • 　High-speed atomic force microscopy and biomolecular processes　T. Uchihashi and T. Ando　Methods Mol. Biol.　736　285-300　2011
  • 　Structural changes in bacteriorhodopsin in response to alternate illumination observed by high-speed atomic force microscopy　M. Shibata, T. Uchihashi, H. Yamashita, H. Kandori, and T. Ando　ANGEWANDTE CHEMIE-INTERNATIONAL EDITION　50　4410 –4413 　2011
  • 　Traffic jams reduce hydrolytic efficiency of cellulase on cellulose surface　K. Igarashi, T. Uchihashi, A. Koivula, M. Wada, S. Kimura, T. Okamoto, M. Penttilä, T. Ando, and M. Samejima　SCIENCE　333　1279-1282 　2011
  • 　Imaging of nucleic acids with atomic force microscopy　Y. L. Lyubchenko, L. S. Shlyakhtenko, and T. Ando　METHODS　54　274-283 　2011
  • 　High-speed Atomic Force Microscopy for Directly Visualizing Dynamic Biomolecular Processes　45　1　22-30　2010
  • 　Direct Observation of Surfactant Aggregate Behavior on a Mica Surface using High-Speed Atomic Force Microscopy　S. Inoue, T. Uchihashi, D. Yamamoto, and T. Ando　CHEMICAL COMMUNICATIONS　47　4974–4976 　2011
  • 　Video imaging of walking myosin V by high-speed atomic force microscopy　N. Kodera, D. Yamamoto, R. Ishikawa, and T. Ando　NATURE　 468　7320　72-76 　2010
  • 　High-speed atomic force microscopy shows dynamic molecular processes in photo-activated bacteriorhodopsin　M. Shibata, H. Yamashita, T. Uchihashi, H. Kandori, and T. Ando　Nature Nanotechnology　5　208-212 　2010
  • 　Deciphering the structure, growth and assembly of amyloid-like fibrils using high-speed atomic force microscopy　P.-E. Milhiet, D. Yamamoto, O. Berthoumieu, P. Dosset, Ch. Le Grimellec, J.-M. Verdier, S. Marchal, and T. Ando　PLos One　5　11　e13240 (8 pp)　2010
  • 　High-speed atomic force microscopy techniques for observing dynamic biomolecular processes　D. Yamamoto, T. Uchihashi, N. Kodera, H. Yamashita, S. Nishikori, T. Ogura, M. Shibata, and T. Ando　METHODS IN ENZYMOLOGY　475　Part B　541-56　2010
  • 　Visualization and structural analysis of the bacterial magnetic organelle magnetosome using atomic force microscopy　D. Yamamoto, A. Taoka, T. Uchihashi, H. Sasaki, H. Watanabe, T. Ando, and Y. Fukumori　PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AME　107　20　9382-9387 　2010
  • 　AAA+ chaperone ClpX regurates dynamics of prokaryotic cytoskeletal protein FtsZ　S. Sugimoto, K. Yamanaka, S. Nishikori, A. Miyagi, T. Ando, and T. Ogura　JOURNAL OF BIOLOGICAL CHEMISTRY　285　9　6648-6657　2010
  • 　Surface topography of membrane domains　M.-C. Giocondi, D. Yamamoto, E. Lesniewska, P.-E. Milhiet, T. Ando, and Ch. Le Grimellec　BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES　1978　703-718 　2010
  • 　High-resolution high-speed contact mode atomic force microscopy movies of purple membrane　I. Casuso, N. Kodera, C. Le Grimellec, T. Ando, and S. Scheuring　BIOPHYSICAL JOURNAL　97　5　1354-1361 　2009
  • 　Dynamics of bacteriorhodopsin 2D crystal observed by high-speed atomic force microscopy.　H. Yamashita, K. Voitchovsky, T. Uchihashi, S. Antoranz Contera, J. F. Ryan, and T. Ando　JOURNAL OF STRUCTURAL BIOLOGY　167　2　153-158　2009
  • 　Single-molecule imaging of a micro-Brownian motion of a chiral helical π-conjugated polymer as a molecular spring driven by thermal fluctuations　K. Shinohara, N. Kodera, and T. Ando　CHEMISTRY LETTERS　38　7　690-691 　2009
  • 　Streptavidin 2D crystal substrates for visualizing biomolecular processes by atomic force microscopy　D. Yamamoto, N. Nagura, S. Omote, M. Taniguchi, and T. Ando　BIOPHYSICAL JOURNAL　97　8　2358–2367 　2009
  • 　High-speed atomic force microscopy for nano-visualization of dynamic biomolecular processes　T. Ando, T. Uchihashi, and T. Fukuma　PROGRESS IN SURFACE SCIENCE　83　337-437 　2008
  • 　Visualization of intrinsically disordered regions of proteins by high-speed atomic force microscopy.　A. Miyagi, Y. Tsunaka, T. Uchihashi, K. Mayanagi, S. Hirose, K. Morikawa, T. Ando　CHEMPHYSCHEM　9　13　1859-1866　2008
  • 　Anisotropic diffusion of point defects in two-dimensional crystal of streptavidin observed by high-speed atomic force microscopy.　D. Yamamoto, T. Uchihashi, N. Kodera, T. Ando　NANOTECHNOLOGY　19　384009-384017　2008
  • 　High-speed AFM and nano-visualization of biomolecular processes　T. Ando, T. Uchihashi, N. Kodera, D. Yamamoto, M. Taniguchi, A. Miyagi, and H. Yamashita　PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY　456　211-225 　2008
  • 　High resonance frequency force microscope scanner using inertia balance support.　T. Fukuma, Y. Okazaki, N. Kodera, T. Uchihahsi, T. Ando　APPLIED PHYSICS LETTERS　92　 ,　243119-243121　2008
  • 　Tip-sample distance control using photo-thermal actuation of a small cantilever for high-speed atomic force microscopy.　H. Yamashita, T. Uchihashi, N. Kodera, A. Miyagi, D. Yamamoto, T. Ando　REVIEW OF SCIENTIFIC INSTRUMENTS　78　 -　83702-83706　2007
  • 　Fabrication of 3D micro-cantilevers based on MBE-grown strained semiconductor layers　H. Iwase, H. Choi, M. Akabori, T. Suzuki, S. Yamada, D. Yamamoto, T. Ando　Physica E: Low-dimensional Systems and Nanostructures 　40　6　2210-2213 　2008
  • 　Control techniques in high-speed atomic force microscopy　Toshio Ando　Proceedings of the American Control Conference　art. no. 4586984　3194-3200 　2008
  • 　High-speed atomic force microscopy for observing dynamic biomolecular processes　T. Ando, T. Uchihashi, N. Kodera, D. Yamamoto, M. Taniguchi, A. Miyagi, H. Yamashita　J. Mol. Recognit.　20　448-458 　2007
  • 　Tip-sample distance control using photo-thermal actuation of a small cantilever for high-speed atomic force microscopy　H. Yamashita, T. Uchihashi, N. Kodera, A. Miyagi, D. Yamamoto, T. Ando　Rev. Sci. Instrum.　78　083702 (5 pp) 　2007
  • 　Single Molecular Imaging of a micro-Brownian Motion and a Bond Scission of a Supramolecular Chiral pai-conjugated.　K. Shinohara, N. Kodera, T. Ando　CHEMISTRY LETTERS　36　1378-1379　2007
  • 　Japan AFM roadmap 2006　S. Morira, H. Yamada, T. Ando　NANOTECHNOLOGY　18　x　084001-084010　2007

Conference Presentations

  • Go to the lab website: http://www.s.kanazawa-u.ac.jp/phys/biophys/invited_talks.htm

Others

  • 　High-speed AFM for studying the dynamic behavior of protein molecules at work.　T. Ando, T. Uchihashi, N. Kodera, A. Miyagi, R. Nakakita, H. Yamashita, M. Matada　3　x　384-392　2005/12
  • 　Review: High-speed atomic force microscopy for observing dynamic biomolecular processes.　T. Ando, T. Uchihashi, N. Kodera, D. Yamamoto, M. Taniguchi, A. Miyagi, H. Yamashita　20　448-458　2007
  • 　Invited Review, high-speed AFM and nanovisualization of biomolecular processes.　T. Ando, T. Uchihashi, N. Kodera, D. Yamamoto, M. Taniguchi, A. Miyagi, and H. Yamashita　456　211-225　2008

Arts and Fieldwork

Patent

○Scan type probe microscope and active damping drive control device(publish date：2008/03)(country：Almost all countries)(No：WO 2008/029561 A1)
○Atomic Force Microscope(publish date：2008/03/01)(country：Almost all countries)(No：WO 2008/029562 A1)
○Scanning probe microscope and molecular structure change observation method.(publish date：2009/07/01)(country：USA)(No：US 7,556,968 B2)
○Scanning unit and scanning microscope having the same(publish date：2004/10/01)(country：USA)(No：US 6,809,306 B2)
○Scanning unit and scanning microscope having the same(publish date：2003/09/01)(country：USA)(No：US 6,617,761 B2)

Theme to the desired joint research

○Development of high-speed scanning probe microscopies

Grant-in-Aid for Scientific Research

○「高速AFMの高度化技術の開発とタンパク質の動作機序解析」(2014-2017)　
○「高速バイオAFMが拓く新構造生物学」(2012-2016)　
○「天然変性蛋白質」(2009-2013)　
○「生命現象の解明に資する革新的高速ＡＦＭの開発」(2008-2012)　
○「最高速AFMが解き明かす生物分子モーターのナノ構造ダイナミクス」(2003-2007)　
○「アクチンに形成される場で無生物は走るか?」(2004-2005)　

Competitive research funding,Contribution

Collaborative research,Consignment study

○Development of a high-speed atomic force microscope(2002-2003)
○Development of 3D high-resolution dynamic imaging instrument(2004-2009)
○Dynamics observation of vesicle adsorption by high-speed AFM(2008-2010)

Classes (Bachelors)

Classes (Graduate Schools)

International Project

International Students

Lecture themes

Others (Social Activities)

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