last modified：2026/04/29
Assistant Professor ICHIKAWA, Takehiko
Faculty, Affiliation
Cancer Research Institute
College and School Educational Field
Division of Nano Life Science, Graduate School of Frontier Science Initiative
Laboratory
Academic Background
【Academic background(Doctoral/Master's Degree)】
Osaka University Doctor Graduate School of Engineering Science Department of Mechanical Science and Bioengineering 200603 Completed
Osaka University Master Graduate School of Engineering Department of Applied Biological Engineering 200303 Completed
【Academic background(Bachelor's Degree)】
Osaka University Division of Applied Science 200103
【Degree】
Doctor of engineering
Career
Kanazawa University Nano Life Science Institute Assistant Professor(2018/07/01-)
Kanazawa Medical University Medical school Assistant professor(2016/09/01-2018/06/30)
RIKEN Researcher(2016/02/01-2016/08/31)
University of Pittsburgh Department of Bioengineering Postdoctoral Fellow(2012/10/01-2015/12/31)
Japan Society for the Promotion of Science Research Fellow (PD)(2010/04/01-2012/12/31)
National Institutes of Natural Sciences National Institute for Basic Biology Postdoctoral researcher(2006/04/01-2010/03/31)
Year & Month of Birth
Academic Society
The Biophysical Society of Japan
Award
○Best Scientific Image/Movie(2011/06/17)
Specialities
Biophysics
Speciality Keywords
Atomic Force Microscope, Light-sheet Fluorescence Microscope, Cell migration, Bioimaging
Research Themes
Development of fusion technology of Atomic Force Microscope and Light Microscope and its application
Development of super-resolution technology using Atomic Force Microscope
Observation of intracellular dynamic structures using nanoendoscopy-AFM
Books
Papers
- Live imaging and quantitative analysis of gastrulation in mouse embryos using light-sheet microscopy and 3D tracking tools Takehiko Ichikawa,Kenichi Nakazato,Philipp J. Keller,Hiroko Kajiura-Kobayashi,Ernst H. K. Stelzer,Atsushi Mochizuki,Shigenori Nonaka Nature Protocols 9 10 2513 2014/10
- Immobilizing single lipid and channel molecules in artificial lipid bilayers with annexin A5 Takehiko Ichikawa,Takaaki Aoki,Yuko Takeuchi,Toshio Yanagida,Toru Ide Langmuir 22 14 6302 2006/07
- Live Imaging of Whole Mouse Embryos during Gastrulation: Migration Analyses of Epiblast and Mesodermal Cells Takehiko Ichikawa,Kenichi Nakazato,Philipp J. Keller,Hiroko Kajiura-Kobayashi,Ernst H. K. Stelzer,Atsushi Mochizuki,Shigenori Nonaka PLoS One 8 7 e64506 2013/07
- A novel method for artificial lipid-bilayer formation Toru Ide,Takehiko Ichikawa Biosensors & Bioelectronics 21 4 672 2005/10
- Immobilizing channel molecules in artificial lipid bilayers for simultaneous electrical and optical single channel recordings Toru Ide,Minako Hirano,Takehiko Ichikawa Cell Signaling Reactions: Single-Molecular Kinetic Analysis 107 2011
- Active K-RAS induces the coherent rotation of epithelial cells: A model for collective cell invasion in vitro. Eishu Hirata,Takehiko Ichikawa(co-first),Shin‐ichi Horike,Etsuko Kiyokawa Cancer Science 109 12 4045 2018/10
- Annexin 5 decreases the diffusion of lipid and channel molecules in an artificial lipid bilayer Takehiko Ichikawa,Yuko Takeuchi,Takaaki Aoki,Toru Ide e-Journal of Surface Science and Nanotechnology 3 213 2005/06/18
- Functional Q beta replicase genetically fusing essential subunits EF-Ts and EF-Tu with beta-subunit Hiroshi Kita,Junghwa Cho,Tomoaki Matsuura,Tomoyuki Nakaishi,Ichiro Taniguchi,Takehiko Ichikawa,Yasuhumi Shima,Itaru Urabe,Tetsuya Yomo JOURNAL OF BIOSCIENCE AND BIOENGINEERING 101 5 421 2006/05
- Epithelium Stratifies via Nucleation and Growth Induced by Foam-Geometric Instability Shuya Fukamachi,Razib Datta,Duligengaowa Wuergezhen,Takehiko Ichikawa,Rei Yagasaki,Shuhei A. Horiguchi,Phoebe Leeaw,Kohei Omachi,Aiko Sada,Itaru Imayoshi,Kentaro Kajiwara,Tetsuya Hiraiwa,Masanobu Oshima,Takeshi Fukuma,Hironobu Fujiwara,Satoru Okuda BioRxiv 2024
- Protocol for live imaging of intracellular nanoscale structures using atomic force microscopy with nanoneedle probes. Takehiko Ichikawa,Mohammad Shahidul Alam,Marcos Penedo,Kyosuke Matsumoto,Sou Fujita,Keisuke Miyazawa,Hirotoshi Furusho,Kazuki Miyata,Chikashi Nakamura,Takeshi Fukuma STAR protocols 4 3 102468 2023/07/22
- Cell penetration efficiency analysis of different atomic force microscopy nanoneedles into living cells. Marcos Penedo,Tetsuya Shirokawa,Mohammad Shahidul Alam,Keisuke Miyazawa,Takehiko Ichikawa,Naoko Okano,Hirotoshi Furusho,Chikashi Nakamura,Takeshi Fukuma Scientific Reports 11 1 7756 2021/04/08
- Chemical fixation creates nanoscale clusters on the cell surface by aggregating membrane proteins. Takehiko Ichikawa,Dong Wang,Keisuke Miyazawa,Kazuki Miyata,Masanobu Oshima,Takeshi Fukuma Communications Biology 5 1 487 2022/05/20
- Probing Nanomechanics by Direct Indentation Using Nanoendoscopy-AFM Reveals the Nuclear Elasticity Transition in Cancer Cells Takehiko Ichikawa,Yohei Kono,Makiko Kudo,Takeshi Shimi,Naoyuki Miyashita,Tomohiro Maesaka,Kojiro Ishibashi,Kundan Sivashanmugan,Takeshi Yoshida,Keisuke Miyazawa,Rikinari Hanayama,Eishu Hirata,Kazuki Miyata,Hiroshi Kimura,Takeshi Fukuma ACS Applied Nano Materials 2025/10/24
- Nanoendoscopy-AFM for Visualizing Intracellular Nanostructures of Living Cells Keisuke Miyazawa,Marcos Penedo,Hirotoshi Furusho,Takehiko Ichikawa,Mohammad Shahidul Alam,Kazuki Miyata,Chikashi Nakamura,Takeshi Fukuma Microscopy and Microanalysis 29 Supplement_1 782 2023/07/22
- Visualizing intracellular nanostructures of living cells by nanoendoscopy-AFM Marcos Penedo,Keisuke Miyazawa,Naoko Okano,Hirotoshi Furusho,Takehiko Ichikawa,Mohammad Shahidul Alam,Kazuki Miyata,Chikashi Nakamura,Takeshi Fukuma Science Advances 7 52 eabj4990 2021/12/24
- The increase in cell volume and nuclear number of the koji-fungus Aspergillus oryzae contributes to its high enzyme productivity Ayaka Itani,Haruto Motomura,Ken Oda,Hideyuki Yamashita,Kanae Sakai,Ken-ichi Kusumoto,Shinsuke Shigeto,Takehiko Ichikawa,Hosain Mohammad Mubarak,Takeshi Fukuma,Takuya Katayama,Jun-ichi Maruyama,Shunsuke Masuo,Naoki Takaya,Norio Takeshita 14 RP107043 2025/09/23
- Roles of the lamin A-specific tail region in the localization to sites of nuclear envelope rupture Yohei Kono,Chan-Gi Pack,Takehiko Ichikawa,Arata Komatsubara,Stephen A Adam,Keisuke Miyazawa,Loïc Rolas,Sussan Nourshargh,Ohad Medalia,Robert D Goldman,Takeshi Fukuma,Hiroshi Kimura,Takeshi Shimi PNAS Nexus 3 12 527 2024/11/21
- Unraveling the host-selective toxic interaction of cassiicolin with lipid membranes and its cytotoxicity. Kien Xuan Ngo,Phuong Doan N Nguyen,Hirotoshi Furusho,Makoto Miyata,Tomomi Shimonaka,Nguyen Ngoc Bao Chau,Nguyen Phuong Vinh,Nguyen Anh Nghia,Tareg Omer Mohammed,Takehiko Ichikawa,Noriyuki Kodera,Hiroki Konno,Takeshi Fukuma,Nguyen Bao Quoc Phytopathology 112 7 1524 2022/05/05
- Condensation-dependent interactome of a chromatin remodeler underlies tumor suppressor activities Yasuhiro Tsukamoto,Atsuki Kawamura,Ayhan Yurtsever,Hidefumi Suzuki,Nichole Marcela Rojas-Chaverra,Hiroki Sato,Daisuke Ino,Takehiko Ichikawa,Weilin Wei,Shojiro Haji,Dominic Chih-Cheng Voon,Akinobu Matsumoto,Kunio Matsumoto,Hidehisa Takahashi,Noriyuki Kodera,Takeshi Fukuma,Yoshihiro Ogawa,Masaaki Nishiyama,Katsuya Sakai Nature Communications 16 9599 2025/10/30
- Influence of nanoendoscopy AFM imaging of intracellular structures on cell proliferation and stress response Mohammad Mubarak Hosain,Mohammad Shahidul Alam,Takehiko Ichikawa,Keisuke Miyazawa,Kazuki Miyata,Takeshi Fukuma Nanoscale 17 39 22958 2025/10/09
- Significant reduction of cell invasiveness in nanoneedle insertion into a living cell with an electron-beam-deposited probe: impacts of probe geometry, speed and vibration Mohammad Shahidul Alam,Marcos Penedo,Takehiko Ichikawa,Mohammad Mubarak Hosain,Kyosuke Matsumoto,Keisuke Miyazawa,Takeshi Fukuma Nanoscale 17 12 7342 2025/03/24
- Chemical Fixation Creates Nanoscale Clusters on the Cell Surface by Aggregating Membrane Proteins Takehiko Ichikawa,Dong Wang,Keisuke Miyazawa,Kazuki Miyata,Masanobu Oshima,Takeshi Fukuma Communications Biology 5 1 487 2021/10/11
- Delayed localization of A-type lamins to the rupture sites in Hutchinson–Gilford progeria syndrome Yohei Kono,Chan-Gi Pack,Takehiko Ichikawa,Arata Komatsubara,Stephen A. Adam,Loïc Rolas,Sussan Nourshargh,Ohad Medalia,Robert D. Goldman,Takeshi Fukuma,Hiroshi Kimura,Takeshi Shimi bioRxiv 2023/09/02
Conference Presentations
- Nanoendoscopy-AFM measurement of the intact intracellular mechanics revealed increased nuclear stiffness in higher metastatic cells(conference：The 61st Annual Meeting of the Biophysics Society of Japan)(2023/11/14)
- Nanoendoscopy-AFM measurement of the intact intracellular mechanics revealed increased nuclear stiffness in higher metastatic cells(2023/09/07)
- Development of the method for observing nanoscale structures outside and inside living cells using atomic force microscopy(2022/09/28)
- Chemical fixation creates nanoscale clusters on the cell surface by aggregating membrane proteins(conference：AFM BioMed Conference 2022)(2022/08/31)
- Chemical fixation creates nanoscale clusters on the cell surface by aggregating membrane proteins(2021/11/25)
- Development of high-resolution observation method of living cell surface in atomic force microscope using porous silicon nitride membrane(conference：The 58h Annual Meeting of the Biophysical Society of Japan)(2020/09/16)
- Development of a method for observing c-Met receptor molecules on lung cancer cell surface with Atomic Force Microscope(conference：The 72nd Annual Meeting of the Japan Society for Cell Biology)(2020/06/10)
- Development of one cell tracking method in MDCK cyst(conference：The 1st Research Conference of Research Society for Cancer 3-D Culture)(2017/12/11)
- Light-sheet fluorescence microscopy (LSFM) and live imaging of early mouse embryo using LSFM(2018/05/18)
- Chemical fixation creates nanoscale clusters on the cell surface by aggregating membrane proteins(2021/12/13)
Others
Arts and Fieldwork
Patent
Theme to the desired joint research
Grant-in-Aid for Scientific Research
○「ナノ内視鏡法を用いた細胞内焦点接着斑の動的微細構造及び形成過程の解明 」(2023-2026)
○「原子間力顕微鏡と化学発光タンパク質を用いたATP超解像イメージング法の開発」(2019-2021)
○「類器官とハイスループットスクリーニングシステムを用いたがん浸潤寄与因子の探索」(2017-2018)
○「MDCKシスト回転運動メカニズムの解明と癌転移への寄与」(2017-2017)
○「中内胚葉の力発生による生体発生への影響」(2013-2013)
○「新規光シート顕微鏡を用いたマウス初期胚における核のエレベーター運動解析」(2010-2012)
Competitive research funding,Contribution
Collaborative research,Consignment study
Classes (Bachelors)
Classes (Graduate Schools)
○Basic Nano Life Sciences(2025)
○Basic Nano Life Sciences(2025)
○Basic Nano Life Sciences(2024)
○Basic Nano Life Sciences(2024)
○Introduction to Nano Science(2024)
○Introduction to Nano Science(2024)
○Basic Nano Life Sciences(2023)
○Basic Nano Life Sciences(2023)
○Introduction to Nano Science(2023)
○Introduction to Nano Science(2023)
○Basic Nano Life Sciences(2022)
○Basic Nano Life Sciences(2022)
○Basic Nano Life Sciences(2021)
○Introduction to Nano Science(2020)
○Introduction to Nano Science(2020)
○Introduction to Nano Science(2020)
○Introduction to Nano Science(2020)
○Basic Nano Life Sciences(2020)
○Basic Nano Life Sciences(2020)
○Basic Nano Life Science(2020)