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Researcher Information

last modified:2020/12/02

Professor YOKOYAMA Shigeru


Faculty, Affiliation

Research Center for Child Mental Development
Professor and Director

College and School Educational Field

Division of Medical Sciences, Graduate School of Medical Science
Division of Medical Science, Graduate School of Medical Science
United Graduate School of Child Depelopment
School of Medicine, College of Medical, Pharmaceutical and Health Sciences


Center for Child Mental Development TEL:076-265-2457 FAX:076-234-4213

Academic Background

【Academic background(Doctoral/Master's Degree)】
Kanazawa University Doctor Graduate School, Division of Medicine Immunobiology 198803 Accomplished credits for doctoral program
【Academic background(Bachelor's Degree)】
Kanazawa University 198503


Year & Month of Birth


Academic Society

Japanese Society of Neurochemistry
The Japanese Biochemical Society
Society for Neuroscience
The Moleular Biology Society of Japan
Japanese Society for Biological Sciences in Space
The Physiological Society of Japan


The Physiological Society of Japan
○Grant-in-aid from Japan Heart Foundation(1988/12)
○Grant-in-aid from Saburo Matsubara Memorial Psychiatry Foundation(1990/03)
○Grant-in-aid from Foundation for Geriatric Medicine(1990/04)
○Grant-in aid from Kowa Life Science Foundation(1994/10)


neurochemistry and neuropharmacology、Neurophysiology/General neuroscience

Speciality Keywords

neurochemistry, neurophysiology, and molecular biology

Research Themes


  •   Inflammatory cytokines in degeneration, regeneration, and maintenance of sciatic nerve. Nova Science Publishers 2011/04
  •  12. Higashida, H., Munesue, T., Yokoyama, S., Hashii, M., Koizumi, K., and Matsushima, A. A missense mutation in CD38 associated with autism spectrum disorder in three pedigrees. InTech 2011/04
  •  Higashida, H., Jin, D., Liu, H.-X., Lopatina, O., Yokoyama, S., Koizumi, K., Hashii, M., Islam, S., Md., Hayashi, K., and Munesue, T. Social memory, mental care and oxytocin secretion, but not vasopressin release, require CD38 in mice. In: Neurobiology of the Parental Brain Academic Press 2008
  •  Higashida, H., Zhang, J.-S., Yokoyama, S., Noda, M., Zhong, Z.-G., Mochida, S., and Egorova, A. Sympathetic potentiation of cyclic ADP-ribose formation in rat cardiac myocytes. Kluwer Academic/Plenum Publishers 2002
  •  Chen, X.-L., Zhong, Z.-G., Yokoyama S., Bark, C., Meister, B., Berggen, P.-O., Roder, J., Higashida, H., and Jeromin, A. Overexpression of rat neuronal calcium sensor-1 in rodent NG108-15 cells enhances functional synapse formation and release of acetylcholine. Cambridge University Press 532 Pt3 649-659 2001/05

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  •  Higashida, H., Yokoyama, S., Hashii, M., and Taketo, M. Slow synaptic responses in neuronal tumor cells: dual regulation of ADP-ribosyl cyclase and inhibition of M-current by muscarinic receptor stimulation. Springer 2000
  •  Three types of cerebellar voltage-gated K+ currents expressed in Xenopus oocytes. Springer 2000
  •  Zhong, Z.-G., Yokoyama, S., Noda, M., and Higashida, H. Role of adhesion molecule L1 in neurite outgrowth and functional synapse formation. Springer 2000
  •  Yokoyama, S., Takeda, H., and Higashida, H. B2 bradykinin receptor in neuronal cells: a genetic aspect. Elsevier 2000
  •  Higashida, H., Yokoyama, S., Hoshi, N., Hashii, M., Noda, M., and Zhong, Z.-G. Preparative study on neuro-muscular synapse formation and hormone-induced calcium mobilizing response in space - a proposal for updated experiments - Proceedings of the Twentieth International Symposium on Space Technology and Science Kokusai Bunken Insatsusha Co., Ltd. 1996


  •  Intracellular calcium elevation induced by extracellular cyclic-ADP-ribose or oxytocin is temperature-sensitive in rodent NG108-15 neuronal cells with or without expression of human oxytocin receptors.  14. Amina, S., Hashii, M., Ma, W.-J., Yokoyama, S., Lopatina, O., Liu, H.-X., Islam, M. S., Graeff, R., Lee, H.-C., and Higashiga, H. JOURNAL OF NEUROENDOCRINOLOGY 22 5 460-466 2010/05
  •  Interleukin-6 upregulates the expression of PMP22 in cultured rat Schwann cells via a JAK2-dependent pathway. Ito, T., Ikeda, K., Tomita, K., and Yokoyama, S. NEUROSCIENCE LETTERS 472 2 104-108 2010/03
  •  Two genetic variants in CD38 in subjects with autism spectrum disorders and controls. 13. Munesue, T.,* Yokoyama, S.,* Nakamura, K.,* Anitha, A.,* Yamada, K.,* Hayashi, K., Asaka, T., Liu, H.-X., Jin, D., Koizumi, K., Islam, Md. S., Huang, J.-J., Ma, W.-J., Kim, U.-H., Kim, S.-J., Park, K., Kim, D., Kikuchi, M., Ono. Y., Nakatani, H., Suda, S., Miyachi, T., Hirai, H., Salmina, A., Pichugina, Y. A., Soumarokov, A. A., Takei, N., Mori, N., Tsujii, M., Sugiyama, T., Yagi, K., Yamagishi, M., Sasaki, T., Yamasue, H., Kato, N., Hashimoto, R., Taniike, M., Hayashi, Y., Hamada, J., Suzuki, S., Ooi, A., Noda, M., Kamiyama, Y., Kido, M. A., Lopatina, O., Hashii, M., Amina, S. S., Malvasi, F., Huang, E. J., Zhang, J.-S., Shimizu, N., Yoshikawa, T., Matsushima, A., Minabe, Y., and Higashida, H. NEUROSCIENCE RESEARCH 67 2 181-191 2010/06
  •  Reply to the letter by Peters, A. M. regarding the article "Quantitative regional blood-flow measurements in exercising skeletal muscle based on 99mTc-pertechnetate clearance". Seto, M., Bunko, H., Shuke, N., Takahashi, K., Sakaibori, Y., Terada, H., Imabayashi, E., Kuji, I., Matsuda, H., and Yokoyama, S. NUCLEAR MEDICINE COMMUNICATIONS 30 4 320-321 2009/04
  •  Locomotor activity, ultrasonic vocalization, oxytocin levels in infant CD38 knockout mice. Liu, H.-X., Lopatina, O., Higashida, C., Tsuji, T., Kato, I., Takasawa-S., Okamoto, H., Yokoyama, S., and Higashida, H. NEUROSCIENCE LETTERS 448 1 67-70 2008/12

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  •  Quantitative regional blood-flow measurements in exercising skeletal muscle based on 99mTc-pertechnetate clearance. Seto, M., Bunko, H., Shuke, N., Takahashi, K., Sakaibori, Y., Terada, H., Imabayashi, E., Kuji, I., Matsuda, H., and Yokoyama, S. NUCLEAR MEDICINE COMMUNICATIONS 29 9 770-774 2008/09
  •  Cyclic ADP-ribose as a universal calcium signal molecule in the nervous system. Higashida, H., Salmina, A. B., Olovyannikova, R., Hashii, M., Yokoyama, S., Koizumi, K., Jin, D., Liu, H.-X., Lopatina, O., Amita, S., Islam, S. Md., Jun, H.-J., and Noda, M. NEUROCHEMISTRY INTERNATIONAL 51 2-4 192-199 2007/09
  •  Oxytocin released from mouse hypothalamus and nerve endings by extracellular application of beta-NAD+ and cyclic ADP ribose. Jin D., Liu, X.-X., Lopatina, O., Hashii, M., Yokoyama, S., Koizumi, K., Amina, S., Shuto, S., Shiraishi, Y., Tanaka, S., and Higashida, H. Nature Protocols (Protocols Network) 10.1038 nprot.2007 214 2007/04
  •  CD38 is critical for social behavior by regulating oxytocin secretion. 22. Jin, D., Liu, H.-X., Hirai, H., Torashima, T., Nagai, T., Lopatina, O., Schayder, N., Yamada, K., Noda, M., Seike, T., Fujita, K., Takasawa, S., Yokoyama, S., Koizumi, K., Shiraishi, M., Tanaka, S., Hashii, M., Yoshihara, T., Higashida, K., Islam, Md., Yamada, N., Hayashi, K., Noguchi, N., Kato, I., Okamoto, H., Matsushima, A., Salmina, A., Munesue, T., Shimizu, N., Mochida, S., Asano, M., and Higashida, H NATURE 446 7131 41-45 2007/03
  •  Bradykinin activates ADP-ribosyl cyclase in neuroblastoma cells: intracellular concentration decrease in NAD and increase in cyclic ADP-ribose. Higashida, H., Salmina, A., Hashii, M., Yokoyama, S., Zhang, J.-S., Noda, M., Zhong, Z.-H., and Jin, D. FEBS LETTERS 580 20 4857-4860 2006/09
  •  Overexpression of constitutively active form of human CD38/ADP-ribosyl cyclase enhances acetylcholine-induced M(KCNQ)-current inhibition in rodent NG108-15 neuroblastoma cells. Higashida, H., Bowden, S. E. H., Yokoyama, S., Egorova, A., Hashii, M., Hoshi, N., Zhang, J.-S., Knijnik, R., Noda, M., Zhong, Z.-G., Takeda, H., Akita, T., Kuba, K., Yamagishi, S., Shimizu, N., Takasawa, S., Okamoto, H., and Robbins, J. NEUROSCIENCE RESEARCH 580 20 4857-4860 2007/03
  •  Induction of tumor necrosis factor-alpha in Schwann cells after gradual elongation of rat sciatic nerve. Hagiwara, N., Ikeda, K., Higashida, H., Tomita, K., and Yokoyama, S. J. Orthop. Sci. 10 6 614-621 2005/11
  •  Induction of interleukin-6 in dorsal root ganglion neurons after gradual elongation of rat sciatic nerve. Osamura, N., Ikeda, K., Ito T., Higashida, H., Tomita, K., and Yokoyama, S. EXPERIMENTAL NEUROLOGY 195 1 61-70 2005/09
  •  Cyclin-dependent kinase 5 activators p35 and p39 facilitate formation of functional synapses. Johansson, J. U., Lilja, L., Chen, X.-L., Higashida, H., Meister, B., Noda, M., Zhong, Z.-G., Yokoyama, S., Berggren, P.-O., and Bark, C. MOLECULAR BRAIN RESEARCH 138 2 215-227 2005/08
  •  Protein kinase C bound with A-kinase anchoring protein is involved in muscarinic receptor-activated modulation of M-type KCNQ potassium channels. Higashida, H., Hoshi, N., Zhang, J.-S., Yokoyama, S., Hashii, M., Duo, J., Noda, M., and Robbins, J. NEUROSCIENCE RESEARCH 51 3 231-234 2005/03
  •  AKAP150 signaling complex promotes suppression of the M-current by muscarinic agonists. Hoshi, N., Zhang, J.-S., Omaki, M., Takeuchi, T., Yokoyama, S., Wanaverbecq, N., Langeberg, L., Yoneda, Y., Scott, J. D., Brown, D. A., and Higashida, H. NATURE NEUROSCIENCE 6 6 564-571 2003/06
  •  Subtype-specific coupling with ADP-ribosyl cyclase of metabotropic glutamate receptors in retina, cervical ganglion and NG108-15 cells. Higashida, H., Mochida, S., Chen, X.-L., Shin, Y., Zhang, J.-S., Noda, M., Hossain, K. Z., Hoshi, N., Hashii, M., Shigemoto, R., Nakanishi, S., Fukuda, Y., and Yokoyama, S. JOURNAL OF NEUROCHEMISTRY 23 4 1148-1158 2003/06
  •  Modulation of the Kv3.1b potassium channel isoform adjusts the fidelity of the firing pattern of auditory neurons. Journal of Neuroscience 23 4 1133-1141 2003/02
  •  Downregulation of vascular endothelial growth factor and its receptors in the kidney in rats with puromycin aminonucleoside nephrosis. Fan, L., Wakayama, T., Yokoyama, S., Amano, O., and Iseki, S. NEPHRON 90 95-102 2002
  •  Enhanced invasiveness of pancreatic adenocarcinoma cells transfected with cationic trypsinogen cDNA. Tajima, S., Ohta, T., Elnemr, A., Yasui, T., Kitagawa, H., Fushida, S., Kayahara, M., Miwa, K., Wakayama, T., Iseki, S., and Yokoyama, S. INTERNATIONAL JOURNAL OF CANCER 95 5 699-704 2001/12
  •  Sympathetic potentiation of cyclic ADP-ribose formation in rat cardiac myocytes. Higashida, H., Egorova, A., Higashida, C., Zhong, Z.-G., Yokoyama, S., Noda, M., and Zhang, J.-S. JOURNAL OF BIOLOGICAL CHEMISTRY 274 47 33348-33354 1999/11
  •  Localization of B2 bradykinin receptor mRNA in the rat retina and sclerocornea. Takeda, H., Kimura, Y., Higashida, H., and Yokoyama, S. IMMUNOPHARMACOLOGY 45 1-3 51-55 1999/12
  •  Expression of Kv1.2 potassium channels in rat sensory ganglia: an immunohistochemical study. ANNALS OF THE NEW YORK ACADEMY OF SCIENCES 868 454-457 1999/04
  •  KCR1, a membrane protein that facilitates functional expression of non-inactivating K+ currents associates with rat EAG voltage-dependent K+ channels. Hoshi, N., Takahashi, H., Shahidullah, M., Yokoyama, S., and Higashida, H. J. Biol. Chem. 273 36 23080-23085 1998/09
  •  Muscarinic acetylcholine receptor subtypes in the human iris. Ishizaka, N., Noda, M., Yokoyama, S., Kawasaki, K., Yamamoto, M., and Higashida, H. BRAIN RESEARCH 787 2 344-347 1998/03
  •  Muscarinic receptor-mediated dual regulation of ADP-ribosyl cyclase in NG108-15 neuronal cell membranes. JOURNAL OF BIOLOGICAL CHEMISTRY 272 50 31272-31277 1997/12
  •  Inositol-1,3,4,5-tetrakisphosphate binding sites in control and ras-transformed NIH/3T3 fibroblasts. Biochem. Biophys. Res. Commun. 239 1 349-352 1997/10
  •  Overexpression of adhesion molecule L1 in NG108-15 neuroblastoma x glioma hybrid cells enhances dibutyryl cAMP-induced neurite outgrowth and functional synapse formation in myotubes. Zhong, Z.-G., Yokoyama, S., Noda, M., and Higashida, H. JOURNAL OF NEUROCHEMISTRY 68 6 2291-2299 1997/06
  •  Ca2+ release and Ca2+ influx in Chinese hamster ovary cells expressing the cloned mouse bradykinin B2 receptor: tyrosine kinase inhibition-sensitive and -insensitive processes. Taketo, M., Yokoyama, S., Kimura, Y., and Higashida, H. BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS 1355 1 89-98 1997/01
  •  Sodium channel alpha-subunit mRNAs I, II, III, NaG, Na6 and hNE (PN1): Different expression pattern in developing rat nervous system. Felts, P. A., Yokoyama, S., Dib-Hajj, S., Black, J. A., and Waxman, S. G. MOLECULAR BRAIN RESEARCH 45 71-82 1997
  •  Bradykinin B2 receptor-induced and inositol tetrakisphosphate-evoked Ca2+ entry is sensitive to a protein tyrosine phosphorylation inhibitor in ras-transformed NIH/3T3 fibroblasts. Hashii, M., Nakashima, S., Yokoyama, S., Enomoto, K-i., Minabe, Y., Nozawa, Y., and Higashida, H. BIOCHEMICAL JOURNAL 319 Pt2 649-656 1996/10
  •  Slow inactivation conserved in heteromultimeric voltage-gated K+ channels between Shaker (Kv1) and Shaw (Kv3) subfamilies. Shahidullah, M., Hoshi, N., Yokoyama, S., Kawamura, T., and Higashida, H. FEBS LETTERS 371 3 301-310 1995/09
  •  Microheterogeneity in heteromultimeric assemblies formed by Shaker (Kv1) and Shaw (Kv3) subfamilies of voltage-gated K+ channels. Shahidullah, M., Hoshi, N., Yokoyama, S., and Higashida, H. PROCEEDINGS OF THE ROYAL SOCIETY OF LONDON SERIES B-BIOLOGICAL SCIENCES 261 1362 309-317 1995/09
  •  Whole-cell analysis of NGK2 (mKv3.1a) K+ channels stably expressed in mouse fibroblast cells. Kawamura, T., Enomoto, K-i., Yokoyama, S., Hoshi, N., Yamashita, J., and Higashida, H. NEUROSCIENCE LETTERS 197 2 164-166 1995/09
  •  Overexpression of choline acetyltransferase reconstitutes discrete acetylcholine release in synapse formation-defective neuroblastoma cells. Zhong, Z.-G., Misawa, H., Furuya, S., Kimura, Y., Noda, M., Yokoyama, S., and Higashida, H. JOURNAL OF PHYSIOLOGY-PARIS 89 3 137-145 1995
  •  Mouse B2 bradykinin receptor gene maps to distal chromosome 12. Taketo, M., Yokoyama, S., Rochelle, J., Kimura, Y., Higashida, H., Taketo, M., and Seldin, M. F. GENOMICS 27 1 222-223 1995/05
  •  B2 bradykinin receptors in NG108-15 cells: cDNA cloning and functional expression. Yokoyama, S., Kimura, Y., Taketo, M., Black, J. A., Ransom, B. R., and Higashida, H. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 200 1 634-641 1994/04
  •  Sodium channel mRNAs in cultured spinal cord astrocytes: in situ hybridization in identified cell types. Black, J. A., Yokoyama, S., Waxman, S. G., Oh, Y., Zur, K. B., Sontheimer, H., Higashida, H., and Ransom, B. R. MOLECULAR BRAIN RESEARCH 23 3 235-245 1994/05
  •  Sodium channel mRNA I, II and III in the CNS: Cell-specific expression. Black, J. A., Yokoyama, S., Higashida, H., Ransom, B. R., and Waxman, S. G. MOLECULAR BRAIN RESEARCH 22 1-4 275-289 1994/04
  •  Coupling of m2 and m4 muscarinic acetylcholine receptor subtypes to Ca2+ -dependent K+ channels in transformed NL308 neuroblastoma x fibroblast hybrid cells. Noda, M., Katayama, M., Brown, D. A., Robbins, J., Marsh, S. J., Ishizaka, N., Fukuda, K., Hoshi, N., Yokoyama, S., and Higashida, H. PROCEEDINGS OF THE ROYAL SOCIETY OF LONDON SERIES B-BIOLOGICAL SCIENCES 251 1332 215-224 1993/03
  •  Charybdotoxin, dendrotoxin and mast cell degranulating peptide block the voltage-activated K+ current of fibroblast cells stably transfected with NGK1 (Kv1.2) K+ channel complementary DNA. Werkman, T. R., Kawamura, T., Yokoyama, S., Higashida, H., and Rogawski, M. A. NEUROSCIENCE 50 4 935-946 1992/10
  •  Potassium channels cloned from neuroblastoma cells display slowly inactivating outward currents in Xenopus oocytes. Ito, Y., Yokoyama, S., and Higashida, H. PROCEEDINGS OF THE ROYAL SOCIETY OF LONDON SERIES B-BIOLOGICAL SCIENCES 248 95-101 1992/05
  •  Phosphoinositides and synaptic function in NG108-15 neuroblastoma x glioma hybrid cells. Higashida, H., Yokoyama, S., Hoshi, N., Myojo, Y., Kawamura, T., Ito, Y., Hashii, M., Sagara, J., and Furuya, K. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY 1991
  •  Post-transcriptional regulation of complement C4 in low C4-producing strain of mouse. Nakayama, K., Pattanakitsakul, S-N., Yokoyama, S., Kimura, H., Nonaka, M., and Takahashi, M. IMMUNOGENETICS 31 5-6 361-367 1990
  •  Bradykinin induces inositol 1,4,5-trisphosphate-dependent hyperpolarization in K+ M-current deficient hybrid NL308 cells: Comparison with NG108-15 neuroblastoma x glioma hybrid cells. Higashida, H., Okano, Y., Hoshi, N., Yada, Y., Yokoyama, S., Asaga, T., Fu, T., and Nozawa, Y. GLIA 3 1 1-12 1990
  •  Potassium channels from NG108-15 neuroblastoma-glioma hybrid cells: Primary structure and functional expression from cDNAs. Yokoyama, S., Imoto, K., Kawamura, T., Higashida, H., Iwabe, N., Miyata, T., and Numa, S. FEBS LETTERS 259 1 37-42 1989/12
  •  Retroviral expression of the human IL2 gene in a murine T cell line results in cell growth autonomy and tumorigenicity. Yamada, G., Kitamura, Y., Sonoda, H., Harada, H., Taki, S., Mulligan, R. C., Osawa, H., Diamantstein, T., Yokoyama, S., and Taniguchi, T. EMBO JOURNAL 6 6 2705-2709 1987/09
  •  Recombination of two homologous MHC class III genes of the mouse (C4 and Slp) that accounts for the loss of testosterone dependence of sex-limited protein expression. Nakayama, K., Nonaka, M., Yokoyama, S., Yu, D. Y., Pattanakitsakul, S-N., and Takahashi, M. JOURNAL OF IMMUNOLOGY 138 2 620-627 1987/01
  •  Identification of 5'-flanking regulatory region responsible for the difference in transcriptional control between mouse C4 and Slp genes. Nonaka, M., Kimura, H., Yu, D. Y., Yokoyama, S., Nakayama, K., and Takahashi, M. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AME 83 7883-7887 1986/10

Conference Presentations


  •  Expression of interleukin-6 mRNA at the dorsal root ganglia -gradual nerve elongation model-. Osamura, N., Ikeda, K., Hagiwara, N., Hinoki, S., Tomita, K., Yokoyama, S., and Higashida, H. pp  59-62 2003
  •  Cell membrane blebbing is related to the CD38 redistribution in NG108-15 hybrid cells. Egorova, A. B., Yokoyama, S., Fukami, H., and Higashida, H. Plenum Publishing 32 3 275-276 2000/06

Arts and Fieldwork


Theme to the desired joint research

Grant-in-Aid for Scientific Research


Competitive research funding,Contribution

Collaborative research,Consignment study

Classes (Bachelors)

Classes (Graduate Schools)

International Project

International Students

Lecture themes

Others (Social Activities)

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