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Researcher Information

last modified：2024/11/23

Associate Professor　Kobayashi, Isao

Mail

Faculty, Affiliation

Faculty of Biological Science and Technology,Institute of Science and Engineering

College and School Educational Field

Division of Natural Sciences, Graduate School of Natural Science and Technology
Course in Aquatic Biology and Environmental Science, School of Biological Science and Technology, College of Science and Engineering

Laboratory

Laboratory of Stem Cell Development 　TEL:076-264-6232　FAX:076-264-6232

Academic Background

【Academic background(Doctoral/Master's Degree)】
Nihon University　Doctor　Graduate School of Veterinary Medicine　2009　Completed
【Academic background(Bachelor's Degree)】
Nihon University　Department of Veterinary Medicine　2006
【Degree】
Ph.D.

Career

Kanazawa University(2020/11-)
Kanazawa University(2018/04-2020/10)
Kanazawa University(2015/11-2018/03)
University of California, San Diego　Department of Molecular Medicine(2015/04-2015/10)
University of California, San Diego　Department of Molecular Medicine(2013/04-2015/03)
University of California, San Diego　Department of Molecular Medicine(2011/10-2013/03)
Keio University(2009/04-2011/09)
Nihon University　Graduate School of Veterinary Medicine(2008/04-2009/03)

Year & Month of Birth

1980/08

Academic Society

International Society for Experimental Hematology (ISEH)

Award

○Christa Muller-Sieburg Award(2013/08)

Specialities

Stem Cell Biology、

Speciality Keywords

Hematopoietic stem cell,Zebrafish,Stem cell niche,Angioblast,Osteoclast

Research Themes

Identification and Characterization of Hematopoietic Stem Cell Niche in The Fish Kidney

Identification and Characterization of Hematopoietic Stem Cell Niche in The Fish Kidney The homeostasis of the blood system is maintained by rare populations of multipotent hematopoietic stem cells (HSCs). HSCs are by far the most widely utilized human stem cells in the clinic in order to treat blood-related disorders, such as leukemia. HSCs are maintained by interactions with cells comprising their microenvironmental niche, which is established in the bone marrow in mammals. Despite the similarity of blood cell subsets, HSCs in teleost fish reside in the urinary organ, kidney, representing a very different environment relative to mammals. We will utilize comparative approaches for the study of HSC niches between zebrafish and mammals. Our ultimate goal is to define the required core components that regulate HSC dormancy, proliferation, and differentiation.

Elucidation of Regulatory Mechanisms Underlying Hematopoietic Stem Cell Development

Elucidation of Regulatory Mechanisms Underlying Hematopoietic Stem Cell Development A major goal of regenerative medicine is to instruct tissue-specific stem cells from induced pluripotent stem cells (iPSCs). Despite decades of efforts, however, generation of HSCs from iPSCs in vitro is not currently possible, indicating that an improved understanding of the native mechanisms of embryonic HSC specification is required. The zebrafish is a powerful genetic model for the study of HSCs. Its transparent embryos allow us to visualize the dynamics of developing HSCs using cell-specific transgenic animals. The movie shown here represents migration of angioblasts (green) along the ventral surface of somites (red). Utilizing “live-imaging” approaches in the zebrafish embryo, we will investigate when, where, and how HSC precursors are regulated by their environmental cells to establish HSC fate during embryogenesis.

Books

  • 　Hirata, Hiromi,Iida, Atsuo　Zebrafish, Medaka, and other small fishes : new model animals in biology, medicine, and beyond　Springer Nature Singapore　2018

Papers

  • 　Extremely low-frequency electromagnetic fields facilitate both osteoblast and osteoclast activity through Wnt/β-catenin signaling in the zebrafish scale　Jingjing Kobayashi-Sun,Isao Kobayashi,Makoto Kashima,Jun Hirayama,Makiko Kakikawa,Sotoshi Yamada,Nobuo Suzuki　Frontiers in Cell and Developmental Biology　12　2024/02/07　
  • 　Super-enhancer trapping by the nuclear pore via intrinsically disordered regions of proteins in squamous cell carcinoma cells.　Masaharu Hazawa,Dini Kurnia Ikliptikawati,Yuki Iwashima,De-Chen Lin,Yuan Jiang,Yujia Qiu,Kei Makiyama,Koki Matsumoto,Akiko Kobayashi,Goro Nishide,Lim Keesiang,Hironori Yoshino,Toshinari Minamoto,Takeshi Suzuki,Isao Kobayashi,Makiko Meguro-Horike,Yan-Yi Jiang,Takumi Nishiuchi,Hiroki Konno,H Phillip Koeffler,Kazuyoshi Hosomichi,Atsushi Tajima,Shin-Ichi Horike,Richard W Wong　Cell Chemical Biology　in press　2023/11
  • 　LSD1 promotes the egress of hematopoietic stem and progenitor cells into the bloodstream during the endothelial-to-hematopoietic transition　Junya Tamaoki,Hiroki Maeda,Isao Kobayashi,Miki Takeuchi,Ken Ohashi,Aniket Gore,Florian Bonkhofer,Roger Patient,Brant M. Weinstein,Makoto Kobayashi　Developmental Biology　501　92　2023/09
  • 　The utility of zebrafish cardiac arrhythmia model to predict the pathogenicity of KCNQ1 variants　Shihe Cui,Kenshi Hayashi,Isao Kobayashi,Kazuyoshi Hosomichi,Akihiro Nomura,Ryota Teramoto,Keisuke Usuda,Hirofumi Okada,Yaowen Deng,Jingjing Kobayashi-Sun,Tetsuo Nishikawa,Hiroshi Furusho,Takekatsu Saito,Hiroaki Hirase,Kunio Ohta,Manabu Fujimoto,Yuki Horita,Takashi Kusayama,Toyonobu Tsuda,Hayato Tada,Takeshi Kato,Soichiro Usui,Kenji Sakata,Noboru Fujino,Atsushi Tajima,Masakazu Yamagishi,Masayuki Takamura　Journal of Molecular and Cellular Cardiology　177　50　2023/04
  • 　The sinusoidal hematopoietic niche is formed by Jam1a via Notch signaling in the zebrafish kidney　Mao Kondo,Koki Kimura,Jingjing Kobayashi-Sun,Shiori Yamamori,Makoto Taniguchi,David Traver,Isao Kobayashi　iScience　26　4　106508　2023/03

show all

  • 　Inhibition of Canonical Wnt Signaling Promotes Ex Vivo Maintenance and Proliferation of Hematopoietic Stem Cells in Zebrafish.　Koki Kimura,Shiori Yamamori,Masaharu Hazawa,Jingjing Kobayashi-Sun,Mao Kondo,Richard W Wong,Isao Kobayashi　Stem cells (Dayton, Ohio)　40　9　831　2022/09/26
  • 　Activation of RANKL-producing cells under simulated microgravity with a three-dimensional clinostat in regenerating goldfish scales　Tatsuki Yamamoto,Mika Ikegame,Kohei Kuroda,Jingjing Kobayashi-Sun,Jun Hirayama,Isao Kobayashi,Ryoya Kawamura,Masato Endo,Yoshiaki Tabuchi,Yukihiro Furusawa,Koji Yachiguchi,Toshio Sekiguchi,Hajime Matsubara,Sachiko Yano,Atsuhiko Hattori,Nobuo Suzuki　Biological Sciences in Space　36　9　2022/07　
  • 　Hematopoietic Cell Isolation by Antibody-Free Flow Cytometry in the Zebrafish Embryo.　Katsuhiro Konno,Jingjing Kobayashi-Sun,Fumio Arai,Isao Kobayashi,Daisuke Sugiyama　Methods in molecular biology (Clifton, N.J.)　2520　171　2022/05/17
  • 　Jagged 2b induces intercellular signaling within somites to establish hematopoietic stem cell fate in zebrafish.　Yukino Wada,Hikaru Tsukatani,Chihiro Kuroda,Yurika Miyazaki,Miku Otoshi,Isao Kobayashi　Development (Cambridge, England)　149　7　2022/04/01
  • 　Non-aqueous, zwitterionic solvent as an alternative for dimethyl sulfoxide in the life sciences　Kosuke Kuroda,Tetsuo Komori,Kojiro Ishibashi,Takuya Uto,Isao Kobayashi,Riki Kadokawa,Yui Kato,Kazuaki Ninomiya,Kenji Takahashi,Eishu Hirata　Communications Chemistry　3　1　2020/11/11　
  • 　Impact of functional studies on exome sequence variant interpretation in early-onset cardiac conduction system diseases.　Kenshi Hayashi,Ryota Teramoto,Akihiro Nomura,Yoshihiro Asano,Manu Beerens,Yasutaka Kurata,Isao Kobayashi,Noboru Fujino,Hiroshi Furusho,Kenji Sakata,Kenji Onoue,David Y Chiang,Tuomas O Kiviniemi,Eva Buys,Patrick Sips,Micah L Burch,Yanbin Zhao,Amy E Kelly,Masanobu Namura,Yoshihito Kita,Taketsugu Tsuchiya,Bunji Kaku,Kotaro Oe,Yuko Takeda,Tetsuo Konno,Masaru Inoue,Takashi Fujita,Takeshi Kato,Akira Funada,Hayato Tada,Akihiko Hodatsu,Chiaki Nakanishi,Yuichiro Sakamoto,Toyonobu Tsuda,Yoji Nagata,Yoshihiro Tanaka,Hirofumi Okada,Keisuke Usuda,Shihe Cui,Yoshihiko Saito,Calum A MacRae,Seiji Takashima,Masakazu Yamagishi,Masa-Aki Kawashiri,Masayuki Takamura　Cardiovascular research　116　13　2116　2020/11/01
  • 　Melatonin suppresses both osteoblast and osteoclast differentiation through repression of epidermal Erk signaling in the zebrafish scale.　Jingjing Kobayashi-Sun,Nobuo Suzuki,Atsuhiko Hattori,Masaaki Yamaguchi,Isao Kobayashi　Biochemical and biophysical research communications　530　4　644　2020/10/01
  • 　Uptake of osteoblast-derived extracellular vesicles promotes the differentiation of osteoclasts in the zebrafish scale　Jingjing Kobayashi-Sun,Shiori Yamamori,Mao Kondo,Junpei Kuroda,Mika Ikegame,Nobuo Suzuki,Kei ichiro Kitamura,Atsuhiko Hattori,Masaaki Yamaguchi,Isao Kobayashi　Communications Biology　3　1　2020/04/23
  • 　Dual role of Jam3b in early hematopoietic and vascular development　Isao Kobayashi,Jingjing Kobayashi-Sun,Yuto Hirakawa,Madoka Ouchi,Koyuki Yasuda,Hiroyasu Kamei,Shigetomo Fukuhara,Masaaki Yamaguchi　Development (Cambridge)　147　1　2020/01/08
  • 　Enrichment of hematopoietic stem/progenitor cells in the zebrafish kidney.　Isao Kobayashi,Mao Kondo,Shiori Yamamori,Jingjing Kobayashi-Sun,Makoto Taniguchi,Kaori Kanemaru,Fumihiko Katakura,David Traver　Scientific reports　9　1　14205　2019/10/02
  • 　Rap1b Promotes Notch-Signal-Mediated Hematopoietic Stem Cell Development by Enhancing Integrin-Mediated Cell Adhesion.　Seung-Sik Rho,Isao Kobayashi,Eri Oguri-Nakamura,Koji Ando,Masakazu Fujiwara,Naomi Kamimura,Hiromi Hirata,Atsuo Iida,Yoshiko Iwai,Naoki Mochizuki,Shigetomo Fukuhara　Developmental cell　49　5　681　2019/06/03
  • 　Anteroposterior molecular registries in ectoderm of the echinus rudiment　Shinya Adachi,Iyo Niimi,Yui Sakai,Fuminori Sato,Takuya Minokawa,Makoto Urata,Atsuko Sehara-Fujisawa,Isao Kobayashi,Masaaki Yamaguchi　Developmental Dynamics　247　12　1297　2018/11/05　
  • 　RANKL, Ephrin-Eph and Wnt10b are key intercellular communication molecules regulating bone remodeling in autologous transplanted goldfish scales　Yuya Tazaki,Kayo Sugitani,Kazuhiro Ogai,Isao Kobayashi,Haruki Kawasaki,Takafumi Aoyama,Nobuo Suzuki,Yoshiaki Tabuchi,Atsuhiko Hattori,Kei ichiro Kitamura　Comparative Biochemistry and Physiology -Part A : Molecular and Integrative Physiology　225　46　2018/06/15
  • 　Effects of low-intensity pulsed ultrasound on osteoclasts: Analysis with gold-fish scales as a model of bone　Taizo Hanmoto,Yoshiaki Tabuchi,Mika Ikegame,Takashi Kondo,Kei-ichiro Kitamura,Masato Endo,Isao Kobayashi,Hiroyuki Mishima,Toshio Sekiguchi,Makoto Urata,Azusa Seki,Sachiko Yano,Atsuhiko Hattori,Nobuo Suzuki　BIOMEDICAL RESEARCH-TOKYO　38　1　71　2017/02/01
  • 　Effects of hyperglycemia on bone metabolism and bone matrix in goldfish scales　Kei-ichiro Kitamura,Tadashi Andoh,Wakana Okesaku,Yuya Tazaki,Kazuhiro Ogai,Kayo Sugitani,Isao Kobayashi,Nobuo Suzuki,Wenxi Chen,Mika Ikegame,Atsuhiko Hattori　COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY　203　152　2017/01
  • 　Isolation and characterization of hematopoietic stem cells in teleost fish　Isao Kobayashi,Fumihiko Katakura,Tadaaki Moritomo　DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY　58　86　2016/05
  • 　Gata2b is a restricted early regulator of hemogenic endothelium in the zebrafish embryo　Emerald Butko,Martin Distel,Claire Pouget,Bart Weijts,Isao Kobayashi,Kevin Ng,Christian Mosimann,Fabienne E. Poulain,Adam McPherson,Chih-Wen Ni,David L. Stachura,Natasha Del Cid,Raquel Espin-Palazon,Nathan D. Lawson,Richard Dorsky,Wilson K. Clements,David Traver　DEVELOPMENT　142　6　1050　2015/03
  • 　Jam1a-Jam2a interactions regulate haematopoietic stem cell fate through Notch signalling　Isao Kobayashi,Jingjing Kobayashi-Sun,Albert D. Kim,Claire Pouget,Naonobu Fujita,Toshio Suda,David Traver　NATURE　512　7514　319　2014/08/21
  • 　Kinetics of CD4(+) and CD8 alpha(+) T-cell subsets in graft-versus-host reaction (GVHR) in ginbuna crucian carp Carassius auratus langsdorfii　Yasuhiro Shibasaki,Flideaki Toda,Isao Kobayashi,Tadaaki Moritomo,Teruyuki Nakanishi　DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY　34　10　1075　2010/10
  • 　Isolation and characterization of endosteal niche cell populations that regulate hematopoietic stem cells　Yuka Nakamura,Fumio Arai,Hiroko Iwasaki,Kentaro Hosokawa,Isao Kobayashi,Yumiko Gomei,Yoshiko Matsumoto,Hiroki Yoshihara,Toshio Suda　BLOOD　116　9　1422　2010/09/02
  • 　Comparative gene expression analysis of zebrafish and mammals identifies common regulators in hematopoietic stem cells.　Kobayashi I,Ono H,Moritomo T,Kano K,Nakanishi T,Suda T　Blood　115　2　e1　2010/01/14　
  • 　In vivo characterization of primitive hematopoietic cells in clonal ginbuna crucian carp (Carassius auratus langsdorfii)　Isao Kobayashi,Hiroko Kusakabe,Hideaki Toda,Tadaaki Moritomo,Tomoko Takahashi,Teruyuki Nakanishi　VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY　126　1-2　74　2008/11/15
  • 　Characterization of teleost phagocyte NADPH oxidase: Molecular cloning and expression analysis of carp (Cyprinus carpio) phagocyte NADPH oxidase　Masayuki Mayumi,Yoko Takeda,Masataka Hoshiko,Ken Serada,Michihiro Murata,Tadaaki Moritomo,Furnio Takizawa,Isao Kobayashi,Kyosuke Araki,Teruyuki Nakanishi,Hideki Sumimoto　MOLECULAR IMMUNOLOGY　45　6　1720　2008/03
  • 　Long-term hematopoietic reconstitution by transplantation of kidney hematopoietic stem cells in lethally irradiated clonal ginbuna crucian carp (Carassius auratus langsdorfii)　Isao Kobayashi,Sawako Kuniyoshi,Kazuyuki Saito,Tadaaki Moritomo,Tomoko Takahashi,Teruyuki Nakanishi　DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY　32　8　957　2008/02/15
  • 　Characterization and localization of side population (SP) cells in zebrafish kidney hematopoietic tissue　Isao Kobayashi,Kazuyuki Saito,Tadaaki Moritomo,Kyosuke Araki,Fumio Takizawa,Teruyuki Nakanishi　BLOOD　111　3　1131　2008/02/01
  • 　Molecular cloning and expression analysis of T-bet in ginbuna crucian carp (Carassius auratus langsdorfii)　Fumio Takizawa,Kyosuke Araki,Isao Kobayashi,Tadaaki Moritomo,Mitsuru Ototake,Teruyuki Nakanishi　MOLECULAR IMMUNOLOGY　45　1　127　2008/01
  • 　Isolation of side population cells from ginbuna carp (Carassius auratus langsdorfii) kidney hematopoietic tissues　Isao Kobayashi,Tadaaki Moritomo,Mituru Ototake,Teruyuki Nakanishi　DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY　31　7　696　2007/11/13
  • 　Demonstration of hematopoietic stem cells in ginbuna carp (Carassius auratus langsdorfii) kidney　Isao Kobayashi,Mayuko Sekiya,Tadaaki Moritomo,Mituru Ototake,Teruyuki Nakanishi　DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY　30　11　1034　2006/02/20

Conference Presentations

  • Developing hematopoietic stem cells acquire adaptability to the final hematopoietic niche(2022/06/01)
  • 母性由来Abcg2aは背側化を抑制して造血・血管発生を促進する(2021/12/02)
  • Jagged-2bは体節内細胞間シグナル伝達を介して造血幹細胞の発生を制御する(2021/12/01)
  • ゼブラフィッシュにおいて古典的Wntシグナルの阻害は造血幹細胞を維持する(2021/12/01)
  • Inhibition of canonical Wnt signaling maintains hematopoietic stem cells in zebrafish(2021/09/16)

show all

  • Jagged-2b triggers intercellular signaling in the somite to establish the hematopoietic stem cell fate in zebrafish(conference：The 50th Annual Scientific Meeting of the ISEH-Society for Hematology and Stem Cells)(2021/08/25)
  • Sinusoidal endothelium is a key component of hematopoietic niches in the zebrafish kidney(2020/12/02)
  • Developing hematopoietic stem cells acquire adaptability to the final hematopoietic niche(2020/11/20)
  • Identification of hematopoietic stem cell niches in the zebrafish kidney(2020/02/14)
  • ゼブラフィッシュ鱗における骨芽細胞由来の細胞外小胞を取り込みは破骨細胞の分化を促進する(2019/12/08)
  • Jag2bは体節においてEphrin A1b – Wnt16を介して造血幹細胞の発生を制御する(2019/12/07)
  • ゼブラフィッシュにおいてIntegrinはlrrc15を介して造血幹細胞発生を制御する(2019/12/07)
  • ゼブラフィッシュ腎臓における造血ニッチの同定(2019/12/07)
  • 発生期の造血幹細胞は最終的な造血環境への適応能を獲得する(2019/12/06)
  • ゼブラフィッシュにおいてIntegrinはlrrc15を介して造血幹細胞の発生を制御する(2019/12/06)
  • Uptake of osteoblast-derived extracellular vesicles facilitates the differentiation of osteoclasts in the zebrafish scale(2019/12/05)
  • Uptake of osteoblast-derived extracellular vesicles facilitates the differentiation of osteoclasts in the zebrafish scale(2019/09/05)
  • The Sinusoidal Endothelium Functions as a Hematopoietic Niche in the Zebrafish Kidney(conference：The 48th Annual Scientific Meeting of the ISEH-Society for Hematology and Stem Cells)(2019/08/23)
  • 血液・血管系の発生機構の解明を目指して(2019/05/14)
  • ゼブラフィッシュ鱗を用いた骨折修復過程におけるライブイメージング解析(2018/11/29)
  • jam1a変異ゼブラフィッシュを用いた造血幹細胞の維持・増殖機構の解明(2018/11/28)
  • ゼブラフィッシュ鱗を用いた骨折修復過程におけるライブイメージング解析(2018/09/14)
  • jam1a変異ゼブラフィッシュを用いた造血幹細胞の維持・増殖機構の解明(2018/09/13)
  • Jam3b regulates hemato-vascular development through the repression of the Erk signaling pathway(conference：The 47th Annual Scientific Meeting of the ISEH-Society for Hematology and Stem Cells)(2018/08/25)
  • 小型魚類の造血システムに学ぶ幹細胞研究の最前線(2017/09/22)
  • Identification of Novel Regulatory Molecules for Hemato-vascular Development in Zebrafish(2017/08/30)
  • Jam1aおよび Jam2aによる接着は造血幹細胞の発生に必須な Notchのシグナル伝達を促進する(2016/12/01)
  • The Somitic Notch Signaling Regulates Hematopoietic Stem Cell Specification(2016/10/06)
  • A Direct Interaction with The Somite Regulates Hematopoietic Stem Cell Fate(2016/08/21)
  • Cell-to-cell interaction between endothelial-hematopoietic precursors and somites regulates developmental specification of hematopoietic stem cells through Notch signaling(conference：The 42nd Annual Scientific Meeting of the ISEH-Society for Hematology and Stem Cells)(2013/08/24)

Others

Arts and Fieldwork

Patent

Theme to the desired joint research

Grant-in-Aid for Scientific Research

○「造血幹細胞の形成過程におけるインテグリン由来シグナル伝達経路の役割解明」(2019-2021)　
○「ウロコ骨折モデルによる破骨細胞産生新規カップリング因子の同定と分泌時相の解明」(2018-2020)　
○「背側大動脈における造血幹細胞の発生動態の解析」(2017-2018)　
○「栄養環境センサーを分子基軸とした脊椎側弯症に対する発症・進行予測技術の開発」(2017-2019)　
○2022「骨芽細胞で作られるホルモンによる破骨細胞の調節機構：骨モデル（ウロコ）による解析」(2020-2022)　
○「異種ドナー生殖細胞の生着・分化をサポートする新たな生殖細胞移植技術の開発」(2021-2023)　

Competitive research funding,Contribution

Collaborative research,Consignment study

Classes (Bachelors)

○Developmental Biology B(2022)
○Developmental Biology C(2022)
○Developmental Biology Lab(2022)
○Experiments in Biology(2022)
○Biology for Education(2022)
○Basic Biology Lab 2(2022)
○Guide to Biological Science B(2022)

Classes (Graduate Schools)

○Developmental Genetics(2022)
○Genomic Systems Biology A(2022)

International Project

International Students

Lecture themes

Others (Social Activities)

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