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Researcher Information

last modified:2017/05/23

Assistant Professor SEO Daisuke

Mail

Faculty, Affiliation

Faculty of Chemistry, Institute of Science and Engineering

College and School Educational Field

Division of Material Chemistry, Graduate School of Natural Science and Technology

Laboratory

Biological Chemistry TEL:076-264-5683 FAX:076-264-5742

Academic Background

【Academic background(Doctoral/Master's Degree)】
Waseda University Doctor 200203 Completed
Waseda University Master 199903 Completed
【Academic background(Bachelor's Degree)】
Waseda University 199703

Career

(2001/04/01-2003/11/30)
Kanazawa University(2003/12/01-)

Year & Month of Birth

1973/05

Academic Society




International Society on Photosyntesis

Award

Specialities

Photoreaction center Electron transfer、Flavoprotein Iron sulfur protein Cytochrome、Bio-related chemistry、Functional biochemistry、Biophysics

Speciality Keywords

phototrophic bacteria, electron transfer, kinetics

Research Themes

Reaction mechanism of ferredoxin-NADPH oxidoreductase from gram positive bacteria

Reaction mechanism of ferredoxin-NADPH oxidoreductase from gram positive bacteria Ferredoxin-NADPH oxidoreductase(FNR) catalyzes a redox reaction between a small iron sulfur protein, ferredoxin(Fd) and NAD(P)H via a noncovalently-bound FAD prosthetic grope. Recently, we have purified a novel type of FNR with high homology to thioredoxin reductases from gram-positive bacteria and green sulfur bacteria. We investigate structure-function relationships of the enzyme with kinetic and spectroscopic measurements and genetic engineering works.

Iron sulfur type photosynthetic reaction center from photosyntetic bacteria

Iron sulfur type photosynthetic reaction center from photosyntetic bacteria Green sulfur bacteria is an anaerobic photosynthetic bacteria containg a type-I(iron-sulfur type) photosynthetic reaction center (RC). We investigate the electron transfer pathways inside RC and peripheric ones involving inorganic sulfur oxidizing proteins, ferredoxin and FNRs.

Investigation of Fd-dependent oxidoreductases in bacteria

Books

Papers

  •  Electron transfer kinetics in purified reaction centers from the green sulfur bacterium Chlorobium tepidum studied by multiple flash excitation BIOCHEMISTRY 38 12124-12137 1999
  •  Purification of ferredoxins and their reaction with purified reaction center complex from the green sulfur bacterium Chlorobium tepidum BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS 1503 377-384 2001
  •  Photoreduction and reoxidation of the three iron-sulfur clusters of reaction centers of green sulfur bacteria BIOPHYSICAL JOURNAL 81 1208-1219 2001
  •  Kinetics of electron transfer between soluble cytochrome c-554 and purified reaction center complex from the green sulfur bacterium Chlorobium tepidum PHOTOSYNTHESIS RESEARCH 71 121-125 2002
  •  Purification and characterization of ferredoxin-NAD(P)+ reductase from the green sulfur bacterium Chlorobium tepidum BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY 1597 123-132 2002

show all

  •  Time-resolved step-scan FTIR investigation on the primary donor of the reaction center from the green sulfur bacterium Chlorobium tepidum PHOTOSYNTHESIS RESEARCH 75 161-169 2003
  •  Purification and characterization of ferredoxin-NADP(+) reductase encoded by Bacillus subtilis yumC ARCHIVES OF MICROBIOLOGY 182 1 80-89 2004/09
  •  Crystallization and preliminary X-ray studies of ferredoxin-NAD(P)+ reductase from Chlorobium tepidum. Muraki N, Seo D, Shiba T, Sakurai T, Kurisu G. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 64 3 186-189 2008/03
  •  Structure and function of the engineered multicopper oxidase CueO from Escherichia coli--deletion of the methionine-rich helical region covering the substrate-binding site. JOURNAL OF MOLECULAR BIOLOGY 373 1 141-152 2007/10
  •  SoxAX binding protein, a novel component of the thiosulfate oxidizing multi-enzyme system in the green sulfur bacterium Chlorobium tepidum. 190 18 6097-6110 2008/09
  •  Studies of interaction of homo-dimeric ferredoxin-NAD(P)+ oxidoreductases of Bacillus subtilis and Rhodopseudomonas palustris, that are closely related to thioredoxin reductases in amino acid sequence, with ferredoxins and pyridine nucleotide coenzymes. Seo D, Okabe S, Yanase M, Kataoka K, Sakurai T. BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY 1794 4  594-601 2009/04
  •  Four-electron reduction of dioxygen by a multicopper oxidase, CueO, and roles of Asp112 and Glu506 located adjacent to the trinuclear copper center. Kataoka K, Sugiyama R, Hirota S, Inoue M, Urata K, Minagawa Y, Seo D, Sakurai T. JOURNAL OF BIOLOGICAL CHEMISTRY 284 21  14405-14413 2009/03
  •  Crystallization and preliminary X-ray studies of ferredoxin-NADP+ oxidoreductase encoded by Bacillus subtilis yumC. Komori H, Seo D, Sakurai T, Higuchi Y. 2010/04
  •  Biochemical studies of a soxF-encoded monomeric flavoprotein purified from the green sulfur bacterium Chlorobaculum tepidum that stimulates in vitro thiosulfate oxidation. Ogawa T, Furusawa T, Shiga M, Seo D, Sakurai H, Inoue K. BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY 2010/04
  •  Asymmetric dimeric structure of ferredoxin-NAD(P)+ oxidoreductase from the green sulfur bacterium Chlorobaculum tepidum: implications for binding ferredoxin and NADP+. Muraki N, Seo D, Shiba T, Sakurai T, Kurisu G. JOURNAL OF MOLECULAR BIOLOGY 2010/09
  •  Crystal structure analysis of Bacillus subtilis ferredoxin-NADP+ oxidoreductase and the structural basis for its substrate selectivity. Komori H, Seo D, Sakurai T, Higuchi Y PROTEIN SCIENCE 19 2279-2290 2010/12
  •   Document Role of the C-terminal extension stacked on the re-face of the isoalloxazine ring moiety of the flavin adenine dinucleotide prosthetic group in ferredoxin-NADP+ oxidoreductase from Bacillus subtilis Seo, D., Asano, T., Komori, H., Sakurai, T.  Plant Physiology and Biochemistry  81 143-148 2014/08
  •  Replacement of Tyr50 stacked on the si-face of the isoalloxazine ring of the flavin adenine dinucleotide prosthetic group modulates Bacillus subtilis ferredoxin-NADP+ oxidoreductase activity toward NADPH  Seo, D., Naito, H., Nishimura, E., Sakurai, T.  Photosynthesis Research  125 1-2 321-328 2015/02
  •  Pre-steady-state kinetic studies of redox reactions catalysed by Bacillus subtilis ferredoxin-NADP+ oxidoreductase with NADP+/NADPH and ferredoxin  Seo, D., Soeta, T., Sakurai, H., Sétif, P., Sakurai, T.  Biochimica et Biophysica Acta - Bioenergetics  1857 6 678-687 2016/06
  •  Kinetics of NADP+/NADPH reduction–oxidation catalyzed by the ferredoxin-NAD(P)+ reductase from the green sulfur bacterium Chlorobaculum tepidum  Seo, D., Kitashima, M., Sakurai, T., Inoue, K.  Photosynthesis Research  2016/07

Conference Presentations

Arts and Fieldwork

Patent

Theme to the desired joint research

Grant-in-Aid for Scientific Research

Classes (Bachelors)

○Introduction to Information Technology(2017)
○Experiments in Basic Chemistry C(2017)
○Experiments in Basic Chemistry C(2016)
○Introduction to Information Technology(2016)

Classes (Graduate Schools)

○Seminar in Material Creation(2017)
○Seminar in Material Creation(2017)
○Seminar in Material Creation(2016)
○Seminar in Material Creation(2016)

International Project

International Students

Lecture themes

Others (Social Activities)

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