last modified：2026/03/10
Assistant Professor MURAI, Kazuhisa
Faculty, Affiliation
Faculty of Health Sciences, Institute of Medical, Pharmaceutical and Health Sciences
College and School Educational Field
Division of Health Sciences, Graduate School of Medical Science
Department of Laboratory Sciences，School of Health Sciences, College of Medical, Pharmaceutical and Health Sciences
Laboratory
Academic Background
Career
Kanazawa University
Kanazawa University
Kanazawa University(2019/09/01-)
Year & Month of Birth
Academic Society
THE JAPAN SOCIETY OF HEPATOLOGY
Award
Specialities
Tumor biology、Virology、Immunology、Molecular biology、Gastroenterology
Speciality Keywords
Research Themes
Construction of a Monocyte Atlas for Patient Stratification and Therapeutic Implementation in Hepatocellular Carcinoma
・ Elucidation of the underlying mechanism of liver cancer immunosuppression based on the understanding of cancer cell metabolism
・ Development of anti-HBV drugs targeting host factors for cccDNA eradication
・ Elucidation of infection immune control mechanism by lipase
Books
Papers
- CEP290 is associated with chromatin accessibility of hepatitis B virus cccDNA. Sadahiro Iwabuchi,Yiang-Yi Li,Takeharu Sakamoto,Tadashi Imafuku,Kazuhisa Murai,Atsushi Tajima,Masao Honda,Shinichi Hashimoto Journal of molecular biology 169700 2026/02/10
- Netrin-1 inhibits the attachment and internalization of Hepatitis B virus for hepatocyte infection. Ying Wang,Kazuhisa Murai,Atsuya Ishida,Narumi Kawasaki,Kazuyuki Kuroki,Ying-Yi Li,Yuga Sato,Yutaro Miura,Kureha Takara,Lianghao Kong,Tetsuro Shimakami,Kouki Nio,Yuichiro Higuchi,Hiroshi Suemizu,Satoru Ito,Hiroshi Yanagawa,Shuichi Kaneko,Taro Yamashita,Masao Honda PLoS pathogens 21 12 e1013776 2025/12
- Wnt/β-Catenin Signaling Regulates Hepatitis B Virus cccDNA Levels. Atsuya Ishida,Sadahiro Iwabuchi,Ying-Yi Li,Kazuhisa Murai,Takayoshi Shirasaki,Kazuyuki Kuroki,Tetsuro Shimakami,Koki Nio,Kazunori Kawaguchi,Tadashi Imafuku,Satoru Ito,Taro Yamashita,Shuichi Kaneko,Hiroshi Yanagawa,Kouji Matsushima,Masao Honda,Shinichi Hashimoto International journal of molecular sciences 26 14 2025/07/19
- Endothelial lipase-binding peptides similar to netrin-1 inhibit hepatitis B virus infection. Mayuko Ide,Noriko Tabata,Kazuhisa Murai,Yuko Yonemura,Ying Wang,Atsuya Ishida,Takayoshi Shirasaki,Shuichi Kaneko,Satoru Ito,Masao Honda,Hiroshi Yanagawa FEBS letters 2025/01/25
- High-Throughput Screening of Antiviral Compounds Using a Recombinant Hepatitis B Virus and Identification of a Possible Infection Inhibitor, Skimmianine Mika Yoshita,Masaya Funaki,Tetsuro Shimakami,Masaki Kakuya,Kazuhisa Murai,Saiho Sugimoto,Shotaro Kawase,Koji Matsumori,Taro Kawane,Tomoki Nishikawa,Asuka Nakamura,Reo Suzuki,Atsuya Ishida,Narumi Kawasaki,Yuga Sato,Ying-Yi Li,Ariunaa Sumiyadorj,Kouki Nio,Hajime Takatori,Kazunori Kawaguchi,Kazuyuki Kuroki,Takanobu Kato,Masao Honda,Taro Yamashita Viruses 16 8 1346 2024/08/22
- Roles Played by DOCK11, a Guanine Nucleotide Exchange Factor, in HBV Entry and Persistence in Hepatocytes. Ying-Yi Li,Kazuhisa Murai,Junyan Lyu,Masao Honda Viruses 16 5 2024/05/08
- Potential utility of l-carnitine for preventing liver tumors derived from metabolic dysfunction-associated steatohepatitis. Junyan Lyu,Hikari Okada,Hajime Sunagozaka,Kazunori Kawaguchi,Tetsuro Shimakami,Kouki Nio,Kazuhisa Murai,Takayoshi Shirasaki,Mika Yoshida,Kuniaki Arai,Tatsuya Yamashita,Takuji Tanaka,Kenichi Harada,Toshinari Takamura,Shuichi Kaneko,Taro Yamashita,Masao Honda Hepatology communications 8 5 2024/05/01
- Hepatitis B virus evades the immune system by suppressing the NF-κB signaling pathway with DENND2A. Mayuko Ide,Noriko Tabata,Yuko Yonemura,Kazuhisa Murai,Ying Wang,Atsuya Ishida,Masao Honda,Shuichi Kaneko,Satoru Ito,Hiroshi Yanagawa Microbiology spectrum 12 3 e0378523 2024/03/05
- Nucleos(t)ide analogs for hepatitis B virus infection differentially regulate the growth factor signaling in hepatocytes Ryogo Shimizu,Kazuhisa Murai,Kensuke Tanaka,Yuga Sato,Naho Takeda,Saki Nakasyo,Takayoshi Shirasaki,Kazunori Kawaguchi,Tetsuro Shimakami,Kouki Nio,Yuki Nakaya,Harumi Kagiwada,Katsuhisa Horimoto,Masashi Mizokami,Shuichi Kaneko,Kazumoto Murata,Taro Yamashita,Masao Honda Hepatology Communications 8 1 2024/01
- Functional involvement of endothelial lipase in hepatitis B virus infection. Takayoshi Shirasaki,Kazuhisa Murai,Atsuya Ishida,Kazuyuki Kuroki,Kazunori Kawaguchi,Ying Wang,Souma Yamanaka,Rio Yasukawa,Narumi Kawasaki,Ying-Yi Li,Tetsuro Shimakami,Ariunaa Sumiyadorj,Kouki Nio,Saiho Sugimoto,Noriaki Orita,Hideo Takayama,Hikari Okada,Phuong Doan Thi Bich,Sadahiro Iwabuchi,Shinichi Hashimoto,Mayuko Ide,Noriko Tabata,Satoru Ito,Kouji Matsushima,Hiroshi Yanagawa,Taro Yamashita,Shuichi Kaneko,Masao Honda Hepatology communications 7 9 2023/09/01
- Hepatitis B Virus Utilizes a Retrograde Trafficking Route via the Trans-Golgi Network to Avoid Lysosomal Degradation. Ying-Yi Li,Kazuyuki Kuroki,Tetsuro Shimakami,Kazuhisa Murai,Kazunori Kawaguchi,Takayoshi Shirasaki,Kouki Nio,Saiho Sugimoto,Tomoki Nishikawa,Hikari Okada,Noriaki Orita,Hideo Takayama,Ying Wang,Phuong Doan Thi Bich,Astuya Ishida,Sadahiro Iwabuchi,Shinichi Hashimoto,Takeshi Shimaoka,Noriko Tabata,Miho Watanabe-Takahashi,Kiyotaka Nishikawa,Hiroshi Yanagawa,Motoharu Seiki,Kouji Matsushima,Taro Yamashita,Shuichi Kaneko,Masao Honda Cellular and molecular gastroenterology and hepatology 15 3 533 2023
- A single hepatitis B virus genome with a reporter allows the entire viral life cycle to be monitored in primary human hepatocytes. Ariunaa Sumiyadorj,Kazuhisa Murai,Tetsuro Shimakami,Kazuyuki Kuroki,Tomoki Nishikawa,Masaki Kakuya,Atsumu Yamada,Ying Wang,Atsuya Ishida,Takayoshi Shirasaki,Shotaro Kawase,Ying-Yi Li,Hikari Okada,Kouki Nio,Kazunori Kawaguchi,Taro Yamashita,Yoshio Sakai,Davaadorj Duger,Eishiro Mizukoshi,Masao Honda,Shuichi Kaneko Hepatology communications 2022/06/12
- Leukocyte cell-derived chemotaxin 2 is an antiviral regulator acting through the proto-oncogene MET. Takayoshi Shirasaki,Satoshi Yamagoe,Tetsuro Shimakami,Kazuhisa Murai,Ryu Imamura,Kiyo-Aki Ishii,Hiroaki Takayama,Yukako Matsumoto,Natsumi Tajima-Shirasaki,Naoto Nagata,Ryogo Shimizu,Souma Yamanaka,Atsushi Abe,Hitoshi Omura,Kazunori Kawaguchi,Hikari Okada,Taro Yamashita,Tomoki Yoshikawa,Kazuhiro Takimoto,Motoko Taharaguchi,Shogo Takatsuka,Yoshitsugu Miyazaki,Toshikatsu Tamai,Yamato Tanabe,Makoto Kurachi,Yasuhiko Yamamoto,Shuichi Kaneko,Kunio Matsumoto,Toshinari Takamura,Masao Honda Nature communications 13 1 3176 2022/06/08
- Guanine nucleotide exchange factor DOCK11-binding peptide fused with a single chain antibody inhibits Hepatitis B Virus infection and replication. Mayuko Ide,Noriko Tabata,Yuko Yonemura,Takayoshi Shirasaki,Kazuhisa Murai,Ying Wang,Atsuya Ishida,Hikari Okada,Masao Honda,Shuichi Kaneko,Nobuhide Doi,Satoru Ito,Hiroshi Yanagawa The Journal of biological chemistry 298 7 102097 2022/06/02
- Establishment of liver tumor cell lines from atherogenic and high fat diet fed hepatitis C virus transgenic mice. Takayoshi Shirasaki,Kazuhisa Murai,Masao Honda,Hikari Okada,Yuika Innami,Atsumu Yamada,Tetsuro Shimakami,Kazunori Kawaguchi,Taro Yamashita,Yoshio Sakai,Shuichi Kaneko Scientific reports 11 1 13021 2021/06/22
- Extended interaction networks with HCV protease NS3-4A substrates explain the lack of adaptive capability against protease inhibitors. Georg Dultz,Tetsuro Shimakami,Markus Schneider,Kazuhisa Murai,Daisuke Yamane,Antoine Marion,Tobias M Zeitler,Claudia Stross,Christian Grimm,Rebecca M Richter,Katrin Bäumer,MinKyung Yi,Ricardo M Biondi,Stefan Zeuzem,Robert Tampé,Iris Antes,Christian M Lange,Christoph Welsch The Journal of biological chemistry 295 40 13862 2020/10/02
- Upregulation of the Long Non-Coding RNA HULC by Hepatitis C Virus and its Regulation of Viral Replication. Juria Kitabayashi,Takayoshi Shirasaki,Tetsuro Shimakami,Tomoaki Nishiyama,Christoph Welsch,Masaya Funaki,Kazuhisa Murai,Ariunaa Sumiyadorj,Hajime Takatori,Kazuya Kitamura,Kazunori Kawaguchi,Kuniaki Arai,Taro Yamashita,Yoshio Sakai,Tatsuya Yamashita,Eishiro Mizukoshi,Masao Honda,Shuichi Kaneko The Journal of infectious diseases 226 3 407 2020/06/09
- MicroRNA-10a Impairs Liver Metabolism in Hepatitis C Virus-Related Cirrhosis Through Deregulation of the Circadian Clock Gene Brain and Muscle Aryl Hydrocarbon Receptor Nuclear Translocator-Like 1. Rika Horii,Masao Honda,Takayoshi Shirasaki,Tetsuro Shimakami,Ryogo Shimizu,Souma Yamanaka,Kazuhisa Murai,Kazunori Kawaguchi,Kuniaki Arai,Tatsuya Yamashita,Yoshio Sakai,Taro Yamashita,Hikari Okada,Mikiko Nakamura,Eishiro Mizukoshi,Shuichi Kaneko Hepatology communications 3 12 1687 2019/12
- Establishment and Characterization of a New Cell Line Permissive for Hepatitis C Virus Infection. Hitoshi Omura,Fanwei Liu,Tetsuro Shimakami,Kazuhisa Murai,Takayoshi Shirasaki,Juria Kitabayashi,Masaya Funaki,Tomoki Nishikawa,Ryotaro Nakai,Ariunaa Sumiyadorj,Takehiro Hayashi,Taro Yamashita,Masao Honda,Shuichi Kaneko Scientific reports 9 1 7943 2019/05/28
- Induction of Selenoprotein P mRNA during Hepatitis C Virus Infection Inhibits RIG-I-Mediated Antiviral Immunity. Kazuhisa Murai,Masao Honda,Takayoshi Shirasaki,Tetsuro Shimakami,Hitoshi Omura,Hirofumi Misu,Yuki Kita,Yumie Takeshita,Kiyo-Aki Ishii,Toshinari Takamura,Takeshi Urabe,Ryogo Shimizu,Hikari Okada,Taro Yamashita,Yoshio Sakai,Shuichi Kaneko Cell host & microbe 25 4 588 2019/04/10
- The osteopontin-CD44 axis in hepatic cancer stem cells regulates IFN signaling and HCV replication. Takayoshi Shirasaki,Masao Honda,Taro Yamashita,Kouki Nio,Tetsuro Shimakami,Ryougo Shimizu,Saki Nakasyo,Kazuhisa Murai,Natsumi Shirasaki,Hikari Okada,Yoshio Sakai,Tokiharu Sato,Tetsuro Suzuki,Katsuji Yoshioka,Shuichi Kaneko Scientific reports 8 1 13143 2018/09/03
- Unexpected Replication Boost by Simeprevir for Simeprevir-Resistant Variants in Genotype 1a Hepatitis C Virus. Kazuhisa Murai,Tetsuro Shimakami,Christoph Welsch,Takayoshi Shirasaki,Fanwei Liu,Juria Kitabayashi,Shiho Tanaka,Masaya Funaki,Hitoshi Omura,Tomoki Nishikawa,Ariunaa Sumiyadorj,Masao Honda,Shuichi Kaneko Antimicrobial agents and chemotherapy 62 7 2018/07
- Peretinoin, an Acyclic Retinoid, Inhibits Hepatitis B Virus Replication by Suppressing Sphingosine Metabolic Pathway In Vitro. Kazuhisa Murai,Takayoshi Shirasaki,Masao Honda,Ryogo Shimizu,Tetsuro Shimakami,Saki Nakasho,Natsumi Shirasaki,Hikari Okada,Yoshio Sakai,Taro Yamashita,Shuichi Kaneko International journal of molecular sciences 19 2 2018/01/23
- Peretinoin, an acyclic retinoid, inhibits hepatocarcinogenesis by suppressing sphingosine kinase 1 expression in vitro and in vivo. Masaya Funaki,Juria Kitabayashi,Tetsuro Shimakami,Naoto Nagata,Yuriko Sakai,Kai Takegoshi,Hikari Okada,Kazuhisa Murai,Takayoshi Shirasaki,Takeru Oyama,Taro Yamashita,Tsuguhito Ota,Yoh Takuwa,Masao Honda,Shuichi Kaneko Scientific reports 7 1 16978 2017/12/05
- Efficient Suppression of Hepatitis C Virus Replication by Combination Treatment with miR-122 Antagonism and Direct-acting Antivirals in Cell Culture Systems. Fanwei Liu,Tetsuro Shimakami,Kazuhisa Murai,Takayoshi Shirasaki,Masaya Funaki,Masao Honda,Seishi Murakami,Minkyung Yi,Hong Tang,Shuichi Kaneko Scientific reports 6 30939 2016/08/03
- Impaired interferon signaling in chronic hepatitis C patients with advanced fibrosis via the transforming growth factor beta signaling pathway. Takayoshi Shirasaki,Masao Honda,Tetsuro Shimakami,Kazuhisa Murai,Takayuki Shiomoto,Hikari Okada,Riuta Takabatake,Akihiro Tokumaru,Yoshio Sakai,Taro Yamashita,Stanley M Lemon,Seishi Murakami,Shuichi Kaneko Hepatology (Baltimore, Md.) 60 5 1519 2014/11
- The acyclic retinoid Peretinoin inhibits hepatitis C virus replication and infectious virus release in vitro. Tetsuro Shimakami,Masao Honda,Takayoshi Shirasaki,Riuta Takabatake,Fanwei Liu,Kazuhisa Murai,Takayuki Shiomoto,Masaya Funaki,Daisuke Yamane,Seishi Murakami,Stanley M Lemon,Shuichi Kaneko Scientific reports 4 4688 2014/04/15
- Hepatic interferon-stimulated genes are differentially regulated in the liver of chronic hepatitis C patients with different interleukin-28B genotypes. Masao Honda,Takayoshi Shirasaki,Tetsuro Shimakami,Akito Sakai,Rika Horii,Kuniaki Arai,Tatsuya Yamashita,Yoshio Sakai,Taro Yamashita,Hikari Okada,Kazuhisa Murai,Mikiko Nakamura,Eishiro Mizukoshi,Shuichi Kaneko Hepatology (Baltimore, Md.) 59 3 828 2014/03
Conference Presentations
- Induction of Selenoprotein P mRNA During Hepatitis C Virus Infection Inhibits RIG-I-Mediated Antiviral Immunity (conference：AASLD)(2019/11/10)
Others
Arts and Fieldwork
Patent
Theme to the desired joint research
Grant-in-Aid for Scientific Research
○「B型肝炎ウイルスRNAを制御する宿主因子の機能解析」(2024-2026)
○「新規モニタリング法の確立によるB型肝炎ウイルスを完全駆除する薬物の探索と同定」(2023-2025)
○「シングルセルマルチオミックス解析による肝がん微小環境の解明」(2022-2023)
○「B型・D型肝炎ウイルス共感染が引き起こす肝疾患の分子生物学的機序の解明」(2020-2023)
○「RIP-Seq法を用いたRIG-IのRNA decoy探索と核酸医薬への応用」(2019-2021)
Competitive research funding,Contribution
Collaborative research,Consignment study
Classes (Bachelors)
○Clinial Practice of Microbiology(2025)
○Molecular Biology(2025)
○Molecular Biology Practice(2025)
○Clinical Microbiology 2(2025)
○Parasitology(2025)
○Clinial Practice of Pathologic Microbiology(2024)
○Molecular Biology(2024)
○Molecular Biology Practice(2024)
○Clinical Microbiology 2(2024)
○Parasitology(2024)
○Gene Diagnostics(2023)
○Clinial Practice of Pathologic Microbiology(2023)
○Lecture and Practice of Virology(2023)
○Molecular Biology(2023)
○Presentation and Debate(2023)
○Pathogenic Microbiology 2(2023)
○Laboratory of Serology and Immunology(2023)
○Parasitology(2023)
○Academic Skill(2023)
○Clinial Practice of Pathologic Microbiology(2022)
○Lecture and Practice of Virology(2022)
○Exercise on Recombinant DNA Technology(2022)
○Parasitology(2022)
○Laboratory of Serology and Immunology(2022)
○Pathogenic Microbiology 2(2022)
○Freshman Seminar I(2021)
○Presentation and Debate (Freshman Seminar II)(2021)
○Introduction to Region-studies(2021)
○Exercise on Recombinant DNA Technology(2021)
○Parasitology(2021)
○Laboratory of Serology and Immunology(2021)
○Lecture and Practice of Virology(2021)
○Clinial Practice of Pathologic Microbiology(2021)
○Pathogenic Microbiology 2(2021)
○Lecture and Practice of Virology(2020)
○Exercise on Recombinant DNA Technology(2020)
○Clinial Practice of Pathologic Microbiology(2020)
○Lecture and Practice of Virology(2019)
○Exercise on Recombinant DNA Technology(2019)
○Clinial Practice of Pathologic Microbiology(2019)
Classes (Graduate Schools)
○An Introduction to Medical Laboratory Sciences:Lec(2025)
○Laboratory Rotation Ⅱ(2025)
○Molecular Biology of Tumor: Lecture(2025)
○Laboratory Rotation I(2025)
○An Introduction to Medical Laboratory Sciences:Lec(2024)
○Laboratory Rotation Ⅱ(2024)
○Molecular Biology of Tumor: Lecture(2024)
○Laboratory Rotation I(2024)
○Molecular Biology of Tumor: Lecture(2023)
○Laboratory Rotation Ｉ(2023)
○Laboratory Rotation Ⅱ(2023)
○An Introduction to Medical Laboratory Sciences:Lec(2022)
○Molecular Biology of Tumor: Lecture(2022)