last modified：2026/09/10
Associate Professor SHIMI, Takeshi
Faculty, Affiliation
WPI Nano Life Science Institute
College and School Educational Field
Laboratory
Nano LSI 306 TEL:076-234-4540
Academic Background
Career
Kanazawa University Nano Life Science Institute, World Premier International Research Center Initiative (WPI) Specially Appointed Associate Professor(2023/07-)
Tokyo Institute of Technology Cell Biology Center, Institute of Innovative Research Specially Appointed Associate Professor(2018/04-2023/05)
Northwestern University, Feinberg School of Medicine Department of Cell and Molecular Biology Research Assistant Professor(2017/08-2019/03)
Northwestern University, Feinberg School of Medicine Department of Biochemistry and Molecular Genetics Research Assistant Professor(2016/05-2017/07)
University of Chicago Department of Human Genetics Staff Scientist(2015/06-2016/04)
Northwestern University, Feinberg School of Medicine Department of Cell and Molecular Biology Research Assistant Professor(2012/02-2015/05)
Northwestern University, Feinberg School of Medicine Department of Cell and Molecular Biology Postdoctoral fellow(2006/04-2012/01)
National Institute of Information and Communications Technology Advanced ICT Research Institute Postdoctoral fellow(2005/04-2006/03)
Year & Month of Birth
Academic Society
Award
Specialities
Cell biology
Speciality Keywords
Nuclear structure and functions,Fluorescence Microscopy,Cell Biology
Research Themes
Books
Papers
- A water-soluble poly(diphenylacetylene)-based photoredox system for visible-light-driven DNA fragmentation Haruka Sakumoto,Yohei Kono,Tsuyoshi Taniguchi,Katsuhiro Maeda,Tatsuya Nishimura,Takeshi Shimi Research Square 2026/09/01
- Nuclear wrinkles result from geometric adaptations to cellular and nuclear morphological changes in epithelial cells Khoa Minh Le,Yohei Kono,Takeshi Shimi,Hiroshi Kimura bioRxiv 2026/08/27
- OptoChaperone─A Biohybrid Tool for Regulating Protein Condensates in Cells and In Vitro Do Thanh Tuan,Motonori Matsusaki,Honoka Ota,Soichiro Kawagoe,Hettimudalige Dilini Nisansala,Munehiro Kumashiro,Noriyoshi Isozumi,Hiroyuki Kumeta,Yohei Kono,Takeshi Shimi,Koichiro Ishimori,Eiichiro Mori,Satoshi Arai,Tomohide Saio Journal of the American Chemical Society 148 16 17429 2026/04/19
- Probing Nanomechanics by Direct Indentation Using Nanoendoscopy-AFM Reveals the Nuclear Elasticity Transition in Cancer Cells Ichikawa, T.,Kono, Y.,Kudo, M.,Shimi, T.,Miyashita, N.,Maesaka, T.,Ishibashi, K.,Sivashanmugan, K.,Yoshida, T.,Miyazawa, K.,Hanayama, R.,Hirata, E.,Miyata, K.,Kimura, H.,Fukuma, T. ACS Applied Nano Materials 8 42 20239 2025/10/12
- Crosstalk between mitotic reassembly and repair of the nuclear envelope Kono, Y.,Shimi, T. Nucleus 15 1 2024/12/31
- Roles of the lamin A-specific tail region in the localization to sites of nuclear envelope rupture Kono, Y.,Pack, C.-G.,Ichikawa, T.,Komatsubara, A.,Adam, S.A.,Miyazawa, K.,Rolas, L.,Nourshargh, S.,Medalia, O.,Goldman, R.D.,Fukuma, T.,Kimura, H.,Shimi, T. Pnas Nexus 3 12 2024/11/21
- Defective nuclear envelope repair in laminopathies Yohei Kono,Takeshi Shimi Journal of Japanese Biochemical Society 96 1 101 2024/02/25
- Epigenetic plasticity safeguards heterochromatin configuration in mammals Kei Fukuda,Takeshi Shimi,Chikako Shimura,Takao Ono,Takehiro Suzuki,Kenta Onoue,Satoko Okayama,Hisashi Miura,Ichiro Hiratani,Kazuho Ikeda,Yasushi Okada,Naoshi Dohmae,Shigenobu Yonemura,Azusa Inoue,Hiroshi Kimura,Yoichi Shinkai Nucleic Acids Research 51 12 6190 2023/05/13
- Nucleoplasmic lamin C rapidly accumulates at sites of nuclear envelope rupture with BAF and cGAS Yohei Kono,Stephen A. Adam,Yuko Sato,Karen L. Reddy,Yixian Zheng,Ohad Medalia,Robert D. Goldman,Hiroshi Kimura,Takeshi Shimi Journal of Cell Biology 221 12 e202201024 2022/10/27
- Superfluid helium nanoscope insert with millimeter working range Naoki Kamiya,Kazuki Kuramoto,Kento Takishima,Tatsuya Yumoto,Haruka Oda,Takeshi Shimi,Hiroshi Kimura,Michio Matsushita,Satoru Fujiyoshi Review of Scientific Instruments 93 10 103703 2022/10/01
- RNA polymerase II condensate formation and association with Cajal and histone locus bodies in living human cells Takashi Imada,Takeshi Shimi,Ai Kaiho,Yasushi Saeki,Hiroshi Kimura Genes to Cells 26 5 298 2021/03/19
- Computational analyses reveal spatial relationships between nuclear pore complexes and specific lamins Mark Kittisopikul,Takeshi Shimi,Meltem Tatli,Joseph Riley Tran,Yixian Zheng,Ohad Medalia,Khuloud Jaqaman,Stephen A. Adam,Robert D. Goldman Journal of Cell Biology 220 4 e202007082 2021/02/11
- Variable immersion microscopy with a high numerical aperture Keita Ishida,Kanta Naruse,Yuta Mizouchi,Yoshihiro Ogawa,Michio Matsushita,Takeshi Shimi,Hiroshi Kimura,Satoru Fujiyoshi Optics Letters 46 4 856 2021/02/10
- Adaptive multiorientation resolution analysis of complex filamentous network images Mark Kittisopikul,Amir Vahabikashi,Takeshi Shimi,Robert D Goldman,Khuloud Jaqaman Bioinformatics 36 20 5093 2020/07/11
- A mosaic of old and young nucleoporins Takeshi Shimi,Hiroshi Kimura Journal of Cell Biology 218 2 385 2019/01/15
- The molecular architecture of lamins in somatic cells Yagmur Turgay,Matthias Eibauer,Anne E. Goldman,Takeshi Shimi,Maayan Khayat,Kfir Ben-Harush,Anna Dubrovsky-Gaupp,K. Tanuj Sapra,Robert D. Goldman,Ohad Medalia Nature 543 7644 261 2017/03/01
- Analyses of the Dynamic Properties of Nuclear Lamins by Fluorescence Recovery After Photobleaching (FRAP) and Fluorescence Correlation Spectroscopy (FCS) Shimi Takeshi,Chan-Gi Pack,Robert D. Goldman Methods in Molecular Biology 99 2016/05/05
- Deleterious assembly of mutant p.S143P lamin A/C causes ER stress in familial dilated cardiomyopathy Gun West,Josef Gullmets,Laura Virtanen,Song Ping Li,Anni Keinänen,Takeshi Shimi,Monika Mauermann,Tiina Heliö,Maija Kaartinen,Laura Ollila,Johanna Kuusisto,John E. Eriksson,Robert D. Goldman,Harald Herrmann,Pekka Taimen Journal of Cell Science 129 14 2732 2016/01/01
- Structural organization of nuclear lamins A, C, B1, and B2 revealed by superresolution microscopy Takeshi Shimi,Mark Kittisopikul,Joseph Tran,Anne E. Goldman,Stephen A. Adam,Yixian Zheng,Khuloud Jaqaman,Robert D. Goldman Molecular Biology of the Cell 26 22 4075 2015/11/05
- Autophagy mediates degradation of nuclear lamina Zhixun Dou,Caiyue Xu,Greg Donahue,Takeshi Shimi,Ji-An Pan,Jiajun Zhu,Andrejs Ivanov,Brian C. Capell,Adam M. Drake,Parisha P. Shah,Joseph M. Catanzaro,M. Daniel Ricketts,Trond Lamark,Stephen A. Adam,Ronen Marmorstein,Wei-Xing Zong,Terje Johansen,Robert D. Goldman,Peter D. Adams,Shelley L. Berger Nature 527 7576 105 2015/10/28
- Gene-rich chromosomal regions are preferentially localized in the lamin B deficient nuclear blebs of atypical progeria cells Katrin Bercht Pfleghaar,Pekka Taimen,Veronika Butin-Israeli,Takeshi Shimi,Sabine Langer-Freitag,Yolanda Markaki,Anne E Goldman,Manfred Wehnert,Robert D Goldman Nucleus 6 1 66 2015/01/02
- TRF2 and lamin A/C interact to facilitate the functional organization of chromosome ends Ashley M. Wood,Jannie M. Rendtlew Danielsen,Catherine A. Lucas,Ellen L. Rice,David Scalzo,Takeshi Shimi,Robert D. Goldman,Erica D. Smith,Michelle M. Le Beau,Steven T. Kosak Nature Communications 5 1 5467 2014/11/17
- Concentration-dependent lamin assembly and its roles in the localization of other nuclear proteins Yuxuan Guo,Youngjo Kim,Takeshi Shimi,Robert D. Goldman,Yixian Zheng Molecular Biology of the Cell 25 8 1287 2014/04/15
- Nuclear Lamins and Oxidative Stress in Cell Proliferation and Longevity Takeshi Shimi,Robert D. Goldman Cancer Biology and the Nuclear Envelope 773 415 2014/01/24
- Interphase phosphorylation of lamin A Vitaly Kochin,Takeshi Shimi,Elin Torvaldson,Stephen A. Adam,Anne Goldman,Chan-Gi Pack,Johanna Melo-Cardenas,Susumu Y. Imanishi,Robert D. Goldman,John E. Eriksson Journal of Cell Science 127 12 2683 2014/01/01
- Meeting report: mitosis and nuclear structure John C. Meadows,Katja Graumann,Melpi Platani,Nina Schweizer,Takeshi Shimi,Paola Vagnarelli,Jesse C. Gatlin Journal of Cell Science 126 22 5087 2013/11/15
- Disruption of lamin B1 and lamin B2 processing and localization by farnesyltransferase inhibitors Stephen A. Adam,Veronika Butin-Israeli,Megan M. Cleland,Takeshi Shimi,Robert D. Goldman Nucleus 4 2 142 2013/03/01
- Mechanical model of blebbing in nuclear lamin meshworks Chloe M. Funkhouser,Rastko Sknepnek,Takeshi Shimi,Anne E. Goldman,Robert D. Goldman,Monica Olvera de la Cruz Proceedings of the National Academy of Sciences 110 9 3248 2013/02/11
- The functions of the nuclear envelope in mediating the molecular crosstalk between the nucleus and the cytoplasm Takeshi Shimi,Veronika Butin-Israeli,Robert D Goldman Current Opinion in Cell Biology 24 1 71 2012/02
- Chromosomal regions associated with prostate cancer risk localize to lamin B‐deficient microdomains and exhibit reduced gene transcription Brian T Helfand,Yuanyuan Wang,Katrin Pfleghaar,Takeshi Shimi,Pekka Taimen,Dale K Shumaker The Journal of Pathology 226 5 735 2012/01/17
- The role of nuclear lamin B1 in cell proliferation and senescence Takeshi Shimi,Veronika Butin-Israeli,Stephen A. Adam,Robert B. Hamanaka,Anne E. Goldman,Catherine A. Lucas,Dale K. Shumaker,Steven T. Kosak,Navdeep S. Chandel,Robert D. Goldman Genes & Development 25 24 2579 2011/12/08
- Simian virus 40 induces lamin A/C fluctuations and nuclear envelope deformation during cell entry Veronika Butin-Israeli,Orly Ben-nun-Shaul,Idit Kopatz,Stephen A. Adam,Takeshi Shimi,Robert D. Goldman,Ariella Oppenheim Nucleus 2 4 320 2011/07
- Nuclear Lamins in Cell Regulation and Disease T. Shimi,V. Butin-Israeli,S. A. Adam,R. D. Goldman Cold Spring Harbor Symposia on Quantitative Biology 75 525 2011/04/05
- Nuclear Lamins T. Dechat,S. A. Adam,P. Taimen,T. Shimi,R. D. Goldman Cold Spring Harbor Perspectives in Biology 2 11 a000547 2010/09/08
- The nucleoporin Nup153 affects spindle checkpoint activity due to an association with Mad1 Yvonne C. Lussi,Dale K. Shumaker,Takeshi Shimi,Birthe Fahrenkrog Nucleus 1 1 71 2010/01
- A progeria mutation reveals functions for lamin A in nuclear assembly, architecture, and chromosome organization Pekka Taimen,Katrin Pfleghaar,Takeshi Shimi,Dorothee Möller,Kfir Ben-Harush,Michael R. Erdos,Stephen A. Adam,Harald Herrmann,Ohad Medalia,Francis S. Collins,Anne E. Goldman,Robert D. Goldman Proceedings of the National Academy of Sciences 106 49 20788 2009/12/08
- The A- and B-type nuclear lamin networks: microdomains involved in chromatin organization and transcription Takeshi Shimi,Katrin Pfleghaar,Shin-ichiro Kojima,Chan-Gi Pack,Irina Solovei,Anne E. Goldman,Stephen A. Adam,Dale K. Shumaker,Masataka Kinjo,Thomas Cremer,Robert D. Goldman Genes & Development 22 24 3409 2008/12/15
- Live cell imaging and electron microscopy reveal dynamic processes of BAF-directed nuclear envelope assembly Tokuko Haraguchi,Tomoko Kojidani,Takako Koujin,Takeshi Shimi,Hiroko Osakada,Chie Mori,Akitsugu Yamamoto,Yasushi Hiraoka Journal of Cell Science 121 15 2540 2008/08/01
- The highly conserved nuclear lamin Ig-fold binds to PCNA: its role in DNA replication Dale K. Shumaker,Liliana Solimando,Kaushik Sengupta,Takeshi Shimi,Stephen A. Adam,Antje Grunwald,Sergei V. Strelkov,Ueli Aebi,M. Cristina Cardoso,Robert D. Goldman The Journal of Cell Biology 181 2 269 2008/04/21
- Nuclear lamins: major factors in the structural organization and function of the nucleus and chromatin Thomas Dechat,Katrin Pfleghaar,Kaushik Sengupta,Takeshi Shimi,Dale K. Shumaker,Liliana Solimando,Robert D. Goldman Genes & Development 22 7 832 2008/04/01
- Alterations in mitosis and cell cycle progression caused by a mutant lamin A known to accelerate human aging Thomas Dechat,Takeshi Shimi,Stephen A. Adam,Antonio E. Rusinol,Douglas A. Andres,H. Peter Spielmann,Michael S. Sinensky,Robert D. Goldman Proceedings of the National Academy of Sciences 104 12 4955 2007/03/20
- Dynamic interaction between BAF and emerin revealed by FRAP, FLIP, and FRET analyses in living HeLa cells Takeshi Shimi,Takako Koujin,Miriam Segura-Totten,Katherine L. Wilson,Tokuko Haraguchi,Yasushi Hiraoka Journal of Structural Biology 147 1 31 2004/07
- Heat‐shock induced nuclear retention and recycling inhibition of importin α Maiko Furuta,Shingo Kose,Makiko Koike,Takeshi Shimi,Yasushi Hiraoka,Yoshihiro Yoneda,Tokuko Haraguchi,Naoko Imamoto Genes to Cells 9 5 429 2004/05/14
- Spectral imaging fluorescence microscopy Tokuko Haraguchi,Takeshi Shimi,Takako Koujin,Noriyo Hashiguchi,Yasushi Hiraoka Genes to Cells 7 9 881 2002/09/10
- Multispectral Imaging Fluorescence Microscopy for Living Cells. Yasushi Hiraoka,Takeshi Shimi,Tokuko Haraguchi Cell Structure and Function 27 5 367 2002
Conference Presentations
- The Role of A-Type Lamins in Repair of Nuclear Envelope Ruptures(conference：The 6th NanoLSI Symposium)(2022/11)
Others
- Intracellular nanoscale observation of E-cadherin mediated cell-cell junctions by nanoendoscopy-AFM Ren Takemura,Yohei Kono,Shuto Nishio,Takeshi Shimi,Satoshi Toda,Takeshi Fukuma The 87th Japan Society of Applied Physics (JSAP) Autumn Meeting 2026 87th 11a-B31-3 2026/09
- Substrate-Modulated Nanoendoscopy-AFM of Nuclear Mechanotransduction in Contractile Cells ALEXIS BOROWIAK,TAKEHIKO ICHIKAWA,YOHEI KONO,TAKESHI SHIMI,MASAMICHI ITO,RYO NITTA,TAKESHI FUKUMA The 87th Japan Society of Applied Physics (JSAP) Autumn Meeting 2026 87th 11a-B31-5 2026/09
- Machine Learning Reveals Polarization-Driven Biomechanical Signatures at Ferroelectric Cell Interfaces ALEXIS BOROWIAK,YOHEI KONO,TAKESHI SHIMI,TAKESHI FUKUMA The 87th Japan Society of Applied Physics (JSAP) Autumn Meeting 2026 87th 10a-C213-1 2026/09
- Domain Wall Engineering: Simulating Nanoscale Electric Field Delivery to Living Cells at Ferroelectric Interfaces ALEXIS BOROWIAK,YOHEI KONO,TAKESHI SHIMI,TAKESHI FUKUMA The 87th Japan Society of Applied Physics (JSAP) Autumn Meeting 2026 87th 10a-B31-5 2026/09
- Visualization of nuclear microviscosity using a fluorescent molecular rotor Yuya Matsuda,Yohei Kono,H.D. Nisansala,Takeshi Shimi,Satoshi Arai The 20th Symposium on Biorelevant Chemistry 2026/09
- Machine Learning-Assisted Analysis of Ferroelectric Surface Effects on Cellular Electromechanics Alexis Borowiak,Yohei Kono,Takeshi Shimi,Takeshi Fukuma IEEE International Symposium on Applications of Ferroelectrics (ISAF) & Piezoresponse Force Microscopy (PFM) (IEEE ISAF-PFM 2026) Paper ID 2201 2026/07
- Decoding Electric Field Effects on Living Cells via Ferroelectric Surfaces—A Machine Learning Approach Alexis Borowiak,Yohei Kono,Takeshi Shimi,Takeshi Fukuma The 2026 Materials Research Society (MRS) Spring Meeting & Exhibit EL10.04.02 2026/04
- Nanoendoscopy-AFM—A Novel Method for In Situ Nanomechanical Characterization of the Cell Nucleus Takehiko Ichikawa,Yohei Kono,Makiko Kudo,Takeshi Shimi,Takeshi Fukuma The 2026 Materials Research Society (MRS) Spring Meeting & Exhibit CH09.02.02 2026/04
- Applying Electrical Fields to Living Cells via the Interface of 180° Ferroelectric Domain Walls—A Nanoscopic Approach Alexis Borowiak,Yohei Kono,Takeshi Shimi,Takeshi Fukuma The 2026 Materials Research Society (MRS) Spring Meeting & Exhibit CH09.01.02 2026/04
- Heterochromatin Contiguous to the Lamina Promotes Nuclear Mechanics Masaki Kawase,Yohei Kono,Takehiko Ichikawa,Hiroshi Kimura,Takeshi Fukuma,Yoichi Shinkai,Takeshi Shimi The 5th Meeting of Cross-Scale Biology at Fukuoka P6 2026/02
- Development of a Genomic DNA Isolation Method by Nanoendoscopy-AFM Takeshi Shimi,Takehiko Ichikawa,Cong Quang Vu,Yohei Kono,Keisuke Miyazawa,Tatsuya Nishimura,Satoshi Arai,Takeshi Fukuma The 5th Meeting of Cross-Scale Biology at Fukuoka P5 2026/02
- Distinct Roles of Lamin A and C in Regulating Nuclear Envelope Repair and Chromatin Organization Yohei Kono,Masaki Kawase,Yoshimasa Takizawa,Miyuki Shimazu,Takiko Daikoku,Yusuke Miyanari,Takeshi Fukuma,Hitoshi Kurumizaka,Yoichi Shinkai,Hiroshi Kimura,Takeshi Shimi 日本分子生物学会年会プログラム・要旨集(Web) 48th 3PS-18 2025/12
- Resistance and Response of Chromatin to Mechanical Stress on the Nucleus Masaki Kawase,Yohei Kono,Takehiko Ichikawa,Hiroshi Kimura,Takeshi Fukuma,Yoichi Shinkai,Takeshi Shimi 日本分子生物学会年会プログラム・要旨集(Web) 48th 3AS-15 2025/12
- Development of chromatin collection method by nanoendoscopy-AFM Takeshi Shimi,Takehiko Ichikawa,Cong Quang Vu,Yohei Kono,Keisuke Miyazawa,Tatsuya Nisimura,Satoshi Arai,Takeshi Fukuma 日本分子生物学会年会プログラム・要旨集(Web) 48th 1MS-17 2025/12
- The Heterochromatin Associated with The Nuclear Lamina Regulates Nuclear Mechanics Masaki Kawase,Yohei Kono,Takehiko Ichikawa,Miyata Kazuki,Hiroshi Kimura,Takeshi Fukuma,Yoichi Shinkai,Takeshi Shimi The 18th Annual Meeting of the Japanese Society for Epigenetics P-68 2025/06
- Development of Genomic DNA Manipulation Method Using Nanoendoscopy-AFM Takeshi Shimi,Takehiko Ichikawa,Cong Quang Vu,Yohei Kono,Keisuke Miyazawa,Tatsuya Nishimura,Satoshi Arai,Takeshi Fukuma The 9th NanoLSI Symposium in conjunction with 19th International Symposium on Macrocyclic and Supramolecular Chemistry 2025 (ISMSC2025) PNA_14 2025/05
- Establishment and analysis of immortalized mouse embryonic fibroblasts lacking all lamins Yohei Kono,Tatsuya Niwa,Yixian Zheng,Stephen A. Adam,Robert D. Goldman,Hiroshi Kimura,Takeshi Shimi 日本細胞生物学会大会講演要旨集 76回 3P20 2024/07
- Comprehensive analysis of A-type lamin tail region for repairing the nuclear envelope 河野洋平,PACK Chan-Gi,市川壮彦,福間剛士,木村宏,志見剛 日本分子生物学会年会プログラム・要旨集(Web) 46th 2023/12
Arts and Fieldwork
Patent
Theme to the desired joint research
Grant-in-Aid for Scientific Research
○「核ラミナとヘテロクロマチンの構造が相互依存する分子メカニズムの解明」(2024-2026)
○「細胞シート工学を用いたラミノパチー疾患モデル構築と核ラミナ修復機構の解明」(2020-2024)
Competitive research funding,Contribution
Collaborative research,Consignment study
Classes (Graduate Schools)
Classes (Bachelors)
○Nano Life Science A(2025)
○Nano Life Science B(2025)
○Nano Life Science B(2024)
○Nano Life Science A(2024)