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last modified：2025/04/02
Professor YOSHIDA Shigeto
Faculty, Affiliation
Faculty of Pharmacy, Institute of Medical, Pharmaceutical and Health Sciences
Professor
College and School Educational Field
Division of Frontier Engineering, Graduate School of Natural Science and Technology
Division of Pharmaceutical Sciences, Graduate School of Medical Science
School of Pharmaceutical Sciences, College of Medical, Pharmaceutical and Health Sciences
School of Pharmacy, College of Medical, Pharmaceutical and Health Sciences
Laboratory
Laboratory of Vaccinology and Applied Immunology TEL:076-234-4463 FAX:076-234-4464
Academic Background
【Academic background(Bachelor's Degree)】
Hokkaido University
【Degree】
doctor of science
doctor of medicine
Career
Jichi Medical University School of Medicine Research Associate(1997/01/01-)
Jichi Medical University School of Medicine Associate Professor(2000/04/01-)
Kanazawa University(2010/10/01-)
Year & Month of Birth
1961/04
Academic Society
Award
Specialities
Parasitology (including sanitary zoology)、Virology、Immunology、Hematology
Speciality Keywords
Infection and Immunology,Vaccinology,Malaria
Research Themes
Development of a novel malaria vaccine based on a non-infectious viral vector
Elucidation of mechanism of malaria vaccine frailness caused by parasite-mediated immune disturbance
Elucidation of malaria parasite-mosquito salivary gland interaction using transgenic mosquitoes
Development of an anti-platelet drug targeted to collagen using a novel anti-platelet aggregation protein derived from mosquito saliva.
Books
Papers
- Comparison between neutralization capacity of antibodies elicited by COVID-19 natural infection and vaccination in Indonesia: A prospective cohort Nurisyah S, Iyori M, Hasyim AA, Sakamoto A, Hashimoto H, Yamagata K, Yamauchi S, Amru K, Zainal KH, Idris I, Yoshida S, Djaharuddin I, Syafruddin D, Bukhari A, Asih PBS, Yusuf Y Antibodies 2023/09/21
- Characterization of the Gene Expression Patterns in the Murine Liver Following Intramuscular Administration of Baculovirus Mitsuhiro Iyori, Ryohei Ogawa, Talha Bin Emran, Shuta Tanbo, Shigeto Yoshida Gene Expression 2020/10/28
- Liver-Directed AAV8 Booster Vaccine Expressing Plasmodium falciparum Antigen Following Adenovirus Vaccine Priming Elicits Sterile Protection in a Murine Model Mohammad Shahnaij, Mitsuhiro Iyori, Hiroaki Mizukami, Mayu Kajino, Iroha Yamagoshi, Intan Syafira, Yenni Yusuf, Ken Fujiwara4, Daisuke S. Yamamoto, Hirotomo Kato, Nobuhiko Ohno, Shigeto Yoshida Front Immunol. 2021/06/23
- A Viral-Vectored Multi-Stage Malaria Vaccine Regimen With Protective and Transmission-Blocking Efficacies. Yusuf Y, Yoshii T, Iyori M, Mizukami H, Fukumoto S, Yamamoto DS, Emran TB, Amelia F, Islam A, Syafira I, Yoshida S. Front Immunol 10 2412 2019/10/15
- Adeno-associated virus as an effective malaria booster vaccine following adenovirus priming. Yusuf Y, Yoshii T, Iyori M, Yoshida M, Mizukami H, Fukumoto S, Yamamoto DS, Alam A, Emran TB, Amelia F, Islam A, Otsuka H, Takashima E, Tsuboi T, Yoshida S. Front Immunol (in press) 2019/04/09
- Down-selecting circumsporozoite protein-based malaria vaccine: a comparison of malaria sporozoite challenge model Amelia F, Iyori M, Emran TB, Yamamoto DS, Genshi K, Otsuka H, Onoue Y, Yusuf Y, Islam A, Yoshida S. Parasite Immunol 2019/03/08
- Anopheline antiplatelet protein from mosquito saliva regulates blood feeding behavior. Islam A, Emran TB, Yamamoto DS, Iyori M, Amelia F, Yusuf Y, Yamaguchi R, Alam MS, Silveira H, Yoshida S. Sci Rep 2019/02/01
- Baculovirus-Induced Fast-Acting Innate Immunity Kills Liver-Stage Plasmodium Emran, T. B. Iyori, M. Ono, Y. Amelia, F. Yusuf, Y. Islam, A. Alam, A. Tamura, M. Ogawa, R. Matsuoka, H. Yamamoto, D. S. Yoshida, S. J Immunol 201 8 2441-2451 2018
- Adenovirus-prime and baculovirus-boost heterologous immunization achieves sterile protection against malaria sporozoite challenge in a murine model Yoshida, Kunitaka Iyori, Mitsuhiro Blagborough, Andrew M. Salman, Ahmed M. Dulal, Pawan Sala, Katarzyna A. Yamamoto, Daisuke S. Khan, Shahid M. Janse, Chris J. Biswas, Sumi Yoshii, Tatsuya Yusuf, Yenni Tokoro, Masaharu Hill, Adrian V. S. Yoshida, Shigeto Scientific Reports 8 1 3896 2018
- Protective efficacy of an IL-12-expressing baculoviral malaria vaccine Iyori M., Blagborough A. M., Sala K. A., Nishiura H., Takagi K., Yoshida S. Parasite Immunol 39 12 2017
- DAF-shielded baculovirus-vectored vaccine enhances protection against malaria sporozoite challenge in mice Iyori, M. Yamamoto, D. S. Sakaguchi, M. Mizutani, M. Ogata, S. Nishiura, H. Tamura, T. Matsuoka, H. Yoshida, S. Malar J 16 1 390 2017
- Plasmodium berghei circumvents immune responses induced by merozoite surface protein 1- and apical membrane antigen 1-based vaccines Yoshida S, Nagumo H, Yokomine T, Araki H, Suzuki A, Matsuoka H PLoS ONE 5 e13727 2010/06
- Functional immunogenicity of baculovirus expressing Pfs25, a human malaria transmission blocking vaccine candidate antigen Mlambo G, Kumar N, Yoshida S Vaccine 28 7025-9 2010/03
- Intranasal and intramuscular immunization with Baculovirus Dual Expression System-based Pvs25 vaccine substantially blocks Plasmodium vivax transmission Blagborough AM, Yoshida S, Sattabongkot J, Tsuboi T, Sinden RE Vaccine 28 6014-20 2010/02
- Baculovirus-based nasal drop vaccine confers complete protection against malaria by natural boosting of vaccine-induced antibodies in mice Yoshida S, Araki H, Yokomine T Infect Immun 78 595-602 2010/01
- Flying Vaccinator; a transgenic mosquito delivers a Leishmania vaccine via blood feeding Yamamoto DS, Nagumo H, Yoshida S Insect Mol Biol 2010/01
- Baculovirus Dual Expression System-based malaria vaccine induces strong protection against Plasmodium berghei sporozoite challenge in mice Yoshida S, Kawasaki M, Hariguchi N, Hirota K, Matsumoto M Infect Immun 77 1782-9 2009/04
- Inhibition of collagen-induced platelet aggregation by anopheline anti-platelet protein, a saliva protein from a malaria vector mosquito Yoshida S, Sudo T, Niimi M, Tao L, Sun B, Kambayashi J, Watanabe H, Enjou L, Matsuoka H Blood 111 2007-14 2008/02
- Hemolytic C-type lectin CEL-III from sea cucumber expressed in transgenic mosquitoes impairs malaria parasite development Yoshida S, Shimada Y, Kondoh D, Kouzuma Y, Ghosh AK, Jacobs-Lorena M, Sinden RE PLoS Pathog 3 e192 2007/08
- Robust salivary gland-specific transgene expression in Anopheles stephensi mosquito Yoshida S, Watanabe H Insect Mol Biol 15 403-10 2006/12
- T cell activation and cytokine production via a bispecific single-chain antibody fragment targeted to blood-stage malaria parasites Yoshida S, Kobayshi T, Matsuoka H, Seki C, Gosnell WL, Chang SP, Ishii A Blood 101 2300-6 2003/05
- Baculovirus virions displaying Plasmodium berghei circumsporozoite protein protects mice against malaria sporozoite infection Yoshida S, Kondoh D, Arai E, Matsuoka H, Seki C, Tanaka T, Okada M, Ishii A Virology 316 161-70 2003/04
- Direct immunization into the liver by gene gun protects against lethal challenge of Plasmodium berghei sporozoite Yoshida S, Kashiwamura S, Hosoya Y, Luo E, Matsuoka H, Ishii A, Fujimura A, Kobayashi E Biochem Biophys Res Commun 271 107-5 2000/04
- A single-chain antibody fragment specific for the Plasmodium berghei ookinete protein Pbs21 confers transmission blockade in the mosquito midgut Yoshida S, Matsuoka H, Luo E, Iwai K, Arai M, Sinden, RE, Ishii A Mol Biochem Parasitol 104 195-204 1999/04
- Development of therapeutic and prophylactic vaccine against Tuberculosis using monkey and transgenic mice models Kita Y, Okada M, Nakajima T, Kanamaru N, Hashimoto S, Nagasawa T, Kaneda Y, Yoshida S, Nishida Y, Nakatani H, Takao K, Kishigami C, Kishigami C, Nishimatsu S, Sekine Y, Takamori Y, McMurray DN, Cruz EC, Tan EV, Abalos RM, Burgos JA, Saunderson P, Sakatani M Hum Vaccin 7 108-114 2011
- DAF-shielded baculovirus-vectored vaccine enhances protection against malaria sporozoite challenge in mice Iyori M., Yamamoto D. S., Sakaguchi M., Mizutani M., Ogata S., Nishiura H., Tamura T., Matsuoka H., Yoshida S. Malaria J 16 1 390 2017
- Novel therapeutic vaccine: granulysin and new DNA vaccine against Tuberculosis. Okada M, Kita Y, Nakajima T, Kanamaru N, Hashimoto S, Nagasawa T, Kaneda Y, Yoshida S, Nishida Y, Nakatani H, Takao K, Kishigami C, Nishimatsu S, Sekine Y, Inoue Y, Matsumoto M, McMurray DN, De la Cruz EC, Tan EV, Abalos RM, Burgos JA, Saunderson P, Sakatani M Hum Vaccin 7 Suppl 6-7
- Novel prophylactic vaccine using a prime-boost method and hemagglutinating virus of Japan-envelope against tuberculosis. Okada M, Kita Y, Nakajima T, Kanamaru N, Hashimoto S, Nagasawa T, Kaneda Y, Yoshida S, Nishida Y, Nakatani H, Takao K, Kishigami C, Nishimatsu S, Sekine Y, Inoue Y, McMurray DN, Sakatani M Clin Dev Immunol 2011 1-11 2011
- Identification of the active region responsible for the anti-thrombotic activity of anopheline anti-platelet protein from a malaria vector mosquito Hayashi H, Kyushiki H, Nagano K, Sudo T, Iyori M, Matsuoka H, Yoshida S Platelets 24 324-332 2013
- Protective efficacy of baculovirus dual expression system vaccine expressing Plasmodium falciparum circumsporozoite protein. Iyori M,Nakaya H, Inagaki K, Pichyangkul S, Yamamoto D, Kawasak M,Kwak K, Mizukoshi M, Goto Y, Matsuoka H, Matsumot M, Yoshida S PLoS ONE 8 e 70819 2013
- The crystal structure of the active domain of Anopheles anti-platelet protein, a powerful anti-coagulant, in complex with an antibody. J Sugiyama K, Iyori M, Sawaguchi A, Akashi S, Tame JR, Park SY, Yoshida S J Biol Chem 289 16303-16312 2014
- Baculovirus-vectored multistage Plasmodium vivax vaccine induces both protective and transmission-blocking immunities against transgenic rodent malaria parasites. Mizutani M, Iyori1, Blagborough AM, Fukumoto S, Funatsu T, Sinden RE, Yoshida S Infect Immun 82 4348-4357 2014
- Reduction of malaria transmission by transgenic mosquitoes expressing an anti-sporozoite antibody in their salivary glands Sumitani M, Kasashima K, Yamamoto DS, Yagi K, Yuda M, Matsuoka H, Yoshida S Insect Mol Biol 22 41-51 2013
- Visualization and live imaging analysis of a mosquito saliva protein in host animal skin using a transgenic mosquito with a secreted luciferase reporter system. Yamamoto D, Yokomine T, Sumitani M, Yagi K, Matsuoka H, Yoshida S Insect Mol Biol. 22 685-693 2013
- The Plasmodium berghei sexual stage antigen PSOP12 induces anti-malarial transmission blocking immunity both in vivo and in vitro Sala1 KA, Nishiura H, Upton LM, Zakutansky SE, Delves MJ, Iyori M, Mizutani M, Sinden RE, Yoshida S, Blagborough AM Vaccine 33 437-445 2015
- Malaria sporozoite protein expression enhances baculovirus-mediated gene transfer to hepatocytes. Tamura T, Kawabata C, Matsushita S, Sakaguchi M, Yoshida S. J Gene Med 18 75-85 2016
- Induction of antisporozite antibodies by biting of transgenic Anopheles stephensi delivering malarial antigen via blood feeding. Yamamoto DS, Sumitani M, Nagumo H, Yoshida S, Matsuoka H. Insect Mol Biol. 21 223-233 2012
- Development of a Plasmodium berghei transgenic parasite expressing the full-length Plasmodium vivax circumsporozoite VK247 protein for testing vaccine efficacy in a murine model. Mizutani M, Fukumoto S, Soubeiga AP, Soga A, Iyori M, Yoshida S Malaria J. 15:251-8 2016
Conference Presentations
- P. falciparum – P.vivax bivalent vaccine development using LC16m8Δ / AAV viral vectors platform achieves sterile protection and transmission blocking(conference：ASTMH Conference)(2023/10/18)
- Humoral immune responses to the central repeat region of PfCSP induced by a vial-vectored plasmodium falciparum vaccine play critical roles in protection in a murine model. (conference：ASTMH Conference)(2023/10/18)
- A Head-to-Head comparative study of the LC16m8Δ/AAV vaccine versus RTS,S/AS01E vaccine in murine models(2023/09/14)
- Conquering Plasmodium vivax: LC16m8Δ/AAV Vaccine Strategy on Protection and Transmission-Blockade(conference：PIM & PRiME 2023)(2023/09/12)
- AAV1 and AAV5 as a malaria booster vaccine following priming with LC16m8Δ(conference：PIM & PRiME 2023)(2023/09/12)
- Evaluation of Multistage Malaria Vaccine Using Transgenic Epitope-Deficient Rodent Malaria Parasite as a Tool for Assessing Mechanism of Protection(conference：PIM & PRiME 2023)(2023/09/12)
- P. falciparum—P. vivax bivalent vaccine development using LC16m8Δ/AAV viral vectors platform(conference：BioMalPar XIX: biology and pathology of the malaria parasite)(2023/05/23)
- Protective efficacy of viral-vectored P. falciparum vaccine against mutated parasites in a murine model(conference：BioMalPar XIX: biology and pathology of the malaria parasite)(2023/05/23)
- The synergy of the multi-stage vaccine against Plasmodium falciparum enhances the reduction of parasite prevalence in a murine model(2023/03/30)
- The effectiveness of a multi-stage vaccine formulation in the malaria vivax transmission-blocking(2023/03/30)
- Plasmodium vivax multistage vaccine based on the heterologous viral-vectored platform achieves sterile protection and transmission blocking(conference：ASTMH 2022 Annual Meeting)(2022/10/30)
- A Novel Malaria Multistage Vaccine Based on Vaccinia virus-Prime/AAV5-Boost Elicits Sterile Protection and Transmission Blocking Efficacy(conference：ASTMH 2022 Annual Meeting)(2022/10/30)
- Pre-erythrocytic and transmission-blocking multi-stage malaria vaccine strategy shows a strong synergic effect to control malaria disease(conference：ASTMH 2022 Annual Meeting)(2022/10/30)
- Optimization of Plasmodium falciparum multistage vaccine based on the heterologous viral-vectored platform in a murine model(conference：ASTMH 2022 Annual Meeting)(2022/10/30)
- Availability of the highly attenuated replication-competent vaccinia virus strain LC16m8Δ as a COVID-19 vaccine platform(2022/09/12)
- A novel COVID-19 vaccine based on a Made-in-Japan live attenuated virus induces neutralizing antibodies against S-pseudotyped virus in a murine model(2021/11/16)
- The mechanism of PfCSP-based malaria vaccines and its application to the viral vectored vaccine platform. (conference：17th International Congress of Immunology.)(2019/10/19)
- A viral-vectored multi-stage malaria vaccine regimen with protective and transmission-blocking efficacies(conference：4th Global Conference and Expo on Vaccines Research & Development)(2020/02/10)
- Transgene mediated inhibition of collagen-induced platelet aggregation by Anopheline Anti-Platelet Protein regulates probing time and blood feeding success in malaria vector mosquito Anopheles stephensi(conference：第73回寄生虫学会西日本支部大会)(2017/10/14)
- Evaluation of the immunogenicity and vaccine efficacy of recombinant truncated protein PfCSP region in a murine malaria model(conference：第73回寄生虫学会西日本支部大会)(2017/10/14)
- Development of viral-vectored Plasmodium vivax circumsporozoite vaccines and its evaluation system in a murine model(2017/10/14)
- マラリア PfCSP タンパクのワクチン抗原としての免疫原性および感染防御効果の検討(conference：日本薬学会第138年会)(2018/03/25)
- コラーゲン誘導性血小板凝集阻害活性を有するハマダラカ唾液タンパク AAPP の機能を喪失すると吸血行動が制限される(conference：日本薬学会第138年会)(2018/03/25)
- アデノウイルスとアデノ随伴ウイルス用いたheterologous prime-boost immunization によるマラリアワクチンの開発研究(2018/03/25)
- ウイルスベクターと組換えタンパクを組み合わせたマラリアワクチンの開発研究(2018/03/25)
- アデノ随伴ウイルスベクターを用いたマラリアワクチン開発研究(2018/03/25)
- 組換えワクシニアウイルス LC16m8Δ のマラリアワクチン効果(2018/03/25)
- ウイルスベクターを基盤としたマラリアワクチンの開発(2018/03/25)
- Development of a novel malaria vaccine based on heterologous prime-boost immunization regimen using adenovirus and adeno-associated virus vectors (conference：14th International congress of parasitology (ICOPA 2018))(2018/08/24)
- Baculovirus-induced innate immunity confers complete protection against Plasmodium pre-erythrocytic stage parasites (conference：14th International congress of parasitology (ICOPA 2018))(2018/08/24)
- 肝臓期マラリア原虫を完全排除可能な非感染性ウイルスベクターに関する研究(conference：第74回日本寄生虫学会西日本支部大会)(2018/10/13)
- 組換えワクシニアウイルスLC16m8Δを基盤としたマラリアワクチンの感染防御効果の検討 (II)(conference：第74回日本寄生虫学会西日本支部大会)(2018/10/13)
- 感染症媒介蚊由来唾液タンパクの種間比較(conference：第74回日本寄生虫学会西日本支部大会)(2018/10/13)
- バキュロウイルス投与による肝臓期マラリア原虫の殺傷メカニズムに関する研究(2018/10/13)
- 組換えワクシニアウイルスLC16m8Δのマラリアワクチンとしての新規アプローチ(2018/10/13)
- 強力な抗肝臓期マラリア原虫効果を発揮する自然免疫応答(conference：第74回日本寄生虫学会西日本支部大会)(2018/10/13)
- Vectored PfCSP Vaccines based on Baculovirus Dual Expression System and AdHu5 induce Strong Protective Efficacy against Transgenic Plasmodium berghei (conference：65th Annual Meeting of American Society of Tropical Medicine & Hygiene)(2016/11/13)
- Potential role of serine protease PfSUB3 for Plasmodium falciparum parasite (2016/03/20)
- Baculovirus-induced innate immunity confers 100% protection against sporozoite challenge in a mouse model (2016/03/20)
- Plasmodium falciparum CSP vaccine based on a heterologous adenoviru-sprime and baculovirus-boost immunization regimen confers sterile protection against transgenic P.berghei sporozoite challenge (conference：The U.S.‐Japan Cooperative Medical Sciences Program Presents the 19th International Conference on Emerging Infectious Diseases (EID))(2017/02/07)
- A hybrid Plasmodium falciparum malaria vaccine based on adenovirus-prime and baculovirus-boost immunization regimen (2016/11/05)
- A hybrid Plasmodium falciparum malaria vaccine based on adenovirus-prime and baculovirus-boost immunization regimen. (2016/09/15)
- Differential antibody response to the Anopheles stephensi AAPP and Plasmodium antigens in individuals naturally exposed to bites of afrotropical malaria vectors (conference：64th Annual Meeting of American Society of Tropical Medicine & Hygiene)(2015/10/25)
- P. falciparum multistage viral-vectored vaccines effective for both sporozoite(conference：第86回日本寄生虫学会)(2017/05/22)
- Protective efficacy of viral vectored Plasmodium falciparum malaria vaccines by the prime-boost immunization regimen(conference：第86回日本寄生虫学会)(2017/05/28)
Others
Arts and Fieldwork
Patent
Theme to the desired joint research
Grant-in-Aid for Scientific Research
○「汎用的次世代ワク チンプラットフォーム -COVID-19への適用-」(2022-2024)
○「感染防御・伝番阻止両機能を発揮する三日熱マラリアワクチン開発研究」(2022-2025)
○「肝臓期マラリア原虫に対するバキュロウイルス自然免疫応答による殺傷メカニズム解析」(2019-2021)
○「プリマキンに替わる抗マラリア自然免疫賦活化薬の開発」(2018-2019)
○「ハマダラカ唾液抗体価を指標とした「マラリア感染危険度」評価法の開発」(2016-2018)
○「多機能性ハイブリッド型ワクチンプラットフォームを基盤としたマラリアワクチンの開発」(2015-2017)
○「ハマダラカ唾液成分ワクチンによるマラリア感染防御効果および吸血行動の解析」(2015-2016)
○「抗蚊唾液抗体価をバイオマーカーとしたマラリアベクターコントロールの評価法開発」(2013-2015)
○「コラーゲンを標的とした新規血小板凝集阻害分子の低分子化と抗血小板薬開発研究」(2012-2013)
○「コラーゲンを標的とした新規血小板凝集阻害分子の低分子化と抗血小板薬開発研究」(2012-2013)
○「トランスジェニック蚊を用いたマラリア原虫—唾液腺の相互作用の解明」(2009-2012)
○「トランスジェニック蚊を用いたハマダラカーマラリア原虫の寄生適応性の解明」(2006-2008)
○「トランスジェニック蚊を用いたハマダラカーマラリア原虫の生物学的適応性の解明」(2003-2005)
○「遺伝子導入によるマラリアを媒介しないハマダラカの作製」(2001-2003)
Competitive research funding,Contribution
Collaborative research,Consignment study
Classes (Bachelors)
○Introduction to Life Science(2017)
○Experiments in Biology 1(2017)
○Host Defense(2017)
○Advanced Molecular Cell Biology(2017)
○Molecular and Cellular Biology 2(2017)
○Medicinal Chemistry(2017)
○Medicinal Chemistry(2016)
○Laboratory Rotation 2(2016)
○Introduction to Life Science(2016)
○Freshman Seminar I(2016)
○Experiments in Biology 1(2016)
○Host Defense(2016)
○Advanced Molecular Cell Biology(2016)
○Molecular and Cellular Biology 2(2016)
Classes (Graduate Schools)
○Control of Immune System(2017)
○Analytical Study on Herbal Medicine(2017)
○Maintenance of Genomic Integrity(2017)
○Advanced Seminar on Biopharmaceutical Sciences(2017)
○Advanced Course of Biopharmaceutical Drug Discovery(2017)
○Topics and Importance of Vaccine Development(2016)
○Introduction of Vaccinology(2016)
International Project
○Japan-UK Research Cooperative Program between JSPS and THe Royal SocietyUniversity of CambridgeUnited Kingdom(2020-2022)United KingdomProject leader
○JSPS-DG-RSTHE JOINT RESEARCH PROJECT Hasanuddin UniversityIndonesia(2017-2019)IndonesiaProject leader