Professor MATSUNAGA Tsukasa
Faculty, Affiliation
Faculty of Pharmacy, Institute of Medical, Pharmaceutical and Health Sciences
College and School Educational Field
Division of Frontier Engineering, Graduate School of Natural Science and Technology
Division of Natural System, Graduate School of Natural Science and Technology
School of Pharmaceutical Sciences, College of Medical, Pharmaceutical and Health Sciences
School of Pharmacy, College of Medical, Pharmaceutical and Health Sciences
Laboratory
Human Molecular Genetics TEL:076-234-4487 FAX:076-234-4427
Academic Background
【Academic background(Doctoral/Master's Degree)】
Kanazawa University Master Graduate School, Division of Pharmaceutical Sciences 198603 Completed
Kanazawa University Doctor Graduate School, Division of Pharmaceutical Sciences 198609 Unfinished course
【Academic background(Bachelor's Degree)】
Kanazawa University 1983
Career
Kanazawa University Faculty of Pharmaceutical Sciences(1986/10/01-1990/11/30)
Kanazawa University Faculty of Pharmaceutical Sciences(1990/12/01-1996/06/30)
Kanazawa University Faculty of Pharmaceutical Sciences(1996/07/01-1997/04/30)
Kanazawa University Faculty of Pharmaceutical Sciences(1997/05/01-2002/03/31)
Kanazawa University Faculty of Pharmaceutical Sciences(2002/04/01-)
Year & Month of Birth
1961/02
Academic Society
Japanese Cancer Association
The Pharmaceutical Society of Japan
Award
Specialities
Molecular biology Functional biochemistry Cell biology、Cell Biology Molecular biology Functional biochemistry、、Risk sciences of radiation and chemicals、Biological pharmacy
Speciality Keywords
DNA repair Hereditary disorder Cancer Signal transduction Cellular response Ultraviolet light
Research Themes
Molecular analysis of cellular responses to DNA damage
Functional analysis of DDB complex in cellular responses to DNA damage
Establishment and application of detection systems for UV-induced DNA damage
Books
Papers
- Topoisomerase I-driven repair of UV-induced damage in NER deficient cells. Saha, L. K., Wakasugi, M., Akter, S., Prasad, R., Wilson, S. H., Shimizu, N., Sasanuma, H., Huang, S. -Y. N., Agama, K., Pommier, Y., Matsunaga, T., Hirota, K., Iwai, S., Nakazawa, Y., Ogi, T. and Takeda, S. Proc. Natl. Acad. Sci. U.S.A. 117 25 14412-14420 2020/06/23
- Spironolactone-induced XPB degradation depends on CDK7 kinase and SCFFBXL18 E3 ligase Ueda, M., Matsuura, K., Kawai, H., Wakasugi, M. and Matsunaga, T. Genes Cells in press 2019/02/14
- PDIP38/PolDIP2 controls the DNA damage tolerance pathways by increasing the relative usage of translesion DNA synthesis over template switching. Tsuda, M., Ogawa, S., Ooka, M., Kobayashi, K., Hirota, K., Wakasugi, M., Matsunaga, T., Sakuma, T., Yamamoto, T., Chikuma, S., Sasanuma, H., Debatisse, M., Doherty, A. J., Fuchs, R. P. and Takeda, S. PLoS One 14 3 e0213383 2019/03/06
- Rapid G0/1 transition and cell cycle progression in CD8+ T cells compared to CD4+ T cells following in vitro stimulation. Mishima, T., Fukaya, S., Toda, S., Ando, Y., Matsunaga, T. and Inobe, M. 61 168-175 2017
- Aquarius is required for proper CtIP expression and homologous recombination repair 69. Sakasai, R., Isono, M., Wakasugi, M., Hashimoto, M., Sunatani, Y., Matsui, T., Shibata, A., Matsunaga, T. and Iwabuchi, K. Sci. Rep. 7 1 13808 2017/10
- Optimized gene silencing by co-expression of multiple shRNAs in a single vector. Ishigaki, Y., Nagao, A. and Matsunaga, T. Methods in Molecular Biology 623 109-121 2010
- Stress-activated MAP kinases JNK and p38 target Cdc25B for degradation. 58. Uchida, S., Yoshioka, K., Kizu, R., Nakagama, H., Matsunaga, T., Ishizaka, Y., Poon, R. Y. C. and Yamashita K. CANCER RESEARCH 69 16 6438-6444 2009/07
- Physical and functional interaction between DDB and XPA in nucleotide excision repair. Wakasugi, M., Kasashima, H., Fukase, Y., Imura, M., Imai, R., Yamada, S., Cleaver, J. E. and Matsunaga, T. NUCLEIC ACIDS RESEARCH 37 2 516-525 2009/02
- A mutation in the uvi4 gene promotes progression of endo-reduplication and confers increased tolerance towards ultraviolet B light. Hase, Y., Trung, K. H., Matsunaga, T., Tanaka, A. PLANT JOURNAL 46 2 317-326 2006/04
- Amino acids C-terminal to the 14-3-3 binding motif in CDC25B affect the efficiency of 14-3-3 binding. Uchida, S., Kubo, A., Kizu, R., Nakagama, H., Matsunaga, T., Ishizaka, Y and Yamashita, K. JOURNAL OF BIOCHEMISTRY 139 4 761-769 2006/04
- Perturbed gap-filling synthesis in nucleotide excision repair causes histone H2AX phosphorylation in human quiescent cells. Matsumoto, M., Yaginuma, K., Igarashi, A., Imura, M., Hasegawa, M., Iwabuchi, K., Date, T., Mori, T., Ishizaki, K., Yamashita, K., Inobe, M. and Matsunaga, T. JOURNAL OF CELL SCIENCE 120 6 1104-1112 2007/03
- DDB1 gene disruption causes a severe growth defect and apoptosis in chicken DT40 cells. Wakasugi, M., Matsuura, K., Nagasawa, A., Fu, D., Shimizu, H., Yamamoto, K., Takeda, S. and Matsunaga, T. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 364 771-777 2007/12
- Isolation of XAB2 complex involved in pre-mRNA splicing, transcription and transcription-coupled repair. Kuraoka, I., Ito, S., Wada, T., Hayashida, M., Lee, L., Saijo, M., Nakatsu, Y., Matsumoto, M., Matsunaga, T., Handa, H., Qin, J., Nakatani, Y. and Tanaka, K. JOURNAL OF BIOLOGICAL CHEMISTRY 283 940-950 2008/01
- A new disorder in UV-induced skin cancer with defective DNA repair distinct from xeroderma pigmentosum or Cockayne syndrome. Hashimoto, S., Egawa, K., Ihn, H., Igarashi, A., Matsunaga, T., Tateishi, S., and Yamaizumi, M. JOURNAL OF INVESTIGATIVE DERMATOLOGY 128 694-701 2008/03
- Multiple shRNA expressions in a single plasmid vector improve RNAi against the XPA gene. Nagao, A., Zhao, X., Takegami, T., Nakagawa, H., Matsui, S., Matsunaga, T. and Ishigaki, Y. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 370 301-305 2008/05
- Cleavage-mediated activation of Chk1 during apoptosis. Matsuura, K., Wakasugi, M., Yamashita, K. and Matsunaga, T. JOURNAL OF BIOLOGICAL CHEMISTRY 283 25485-25491 2008/09
- Damaged DNA-binding protein DDB stimulates the excision of cyclobutane pyrimidine dimers in vitro in concert with XPA and replication protein A. J. Biol. Chem. 276 18 15434-15440 2001/05
- DDB accumulates at DNA damage sites immediately after UV irradiation and directly stimulates nucleotide excision repair. J. Biol. Chem. 277 3 1637-1640 2002/01
- Effects of photoreactivation of cyclobutane pyrimidine dimers and pyrimidine (6-4) pyrimidone photoproducts on ultraviolet mutagenesis in SOS-induced repair-deficient Escherichia coli. MUTAGENESIS 16 1 1-6 2001/01
- In situ visualization of ultraviolet light-induced DNA damage repair in locally irradiated human fibroblasts. J. Invest. Dermatol. 117 5 1156-1161 2001/11
- Postnatal growth failure, short life span, and early onset of cellular senescence and subsequent immortalization in mice lacking the xeroderma pigmentosum group G gene. Mol. Cell. Biol. 19 3 2366-2372 1999/03
- Respective roles of cyclobutane pyrimidine dimers, (6-4)photoproducts, and minor photoproducts in ultraviolet mutagenesis of repair-deficient xeroderma pigmentosum A cells. Cancer Res. 60 6 1729-1735 2000/03
- cDNA cloning of the chicken DDB1 gene encoding the p127 subunit of damaged DNA-binding protein. GENES & GENETIC SYSTEMS 78 169-177 2003/04
- Ultraviolet-sensitive syndrome cells are defective in transcription-coupled repair of cyclobutane pyrimidine dimers. MUTATION RESEARCH-DNA REPAIR 1 629-643 2002/08
- A gene for a Class II DNA photolyase from Oryza sativa: cloning of the cDNA by dilution-amplification. MOLECULAR GENETICS AND GENOMICS 269 4 508-516 2003/07
- Antibodies specific for (6-4) DNA photoproducts: cloning, antibody modeling and construction of a single-chain Fv derivative. Biochim. Biophys. Acta 1385 1 17-32 1998/06
- Amplified UvrA protein can ameliorate the ultraviolet sensitivity of an Escherichia coli recA mutant. Mutation Res. 487 3-4 149-156 2001/12
- Binding of 14-3-3beta but not 14-3-3sigma controls the cytoplasmic localization of CDC25B: binding site preferences of 14-3-3 subtypes and the subcellular localization of CDC25B. JOURNAL OF CELL SCIENCE 117 14 3011-3020 2004/06
- Identification of the XPG region that causes the onset of Cockayne syndrome by using Xpg mutant mice generated by the cDNA-mediated knock-in method. MOLECULAR AND CELLULAR BIOLOGY 24 9 3712-3719 2004/05
- Nuclear export signal in CDC25B. Biochem. Biophys. Res. Commun. 316 1 226-232 2004/03
- Characterization of pathways dependent on the uvde, uvrA1, or uvrA2 gene product for ultraviolet resistance in Deinococcus radiodurans. Tanaka, M., Narumi, I., Funayama, T., Kikuchi, M., Watanabe, H., Matsunaga, T., Nikaido, O. and Yamamoto, K. JOURNAL OF BACTERIOLOGY 187 11 3693-3697 2005/06
- Functional and physical interactions between ERCC1 and MSH2 for resistance to cis-platinum in mammalian cells. DNA Repair 3 2 135-143 2004/02
- Human NTH1 physically interacts with p53 and proliferating cell nuclear antigen. Biochemical and Biophysical Research Communications 321 1 183-191 2004/08
- Disruption of the AtREV3 gene causes hypersensitivity to ultraviolet B light and gamma-rays in Arabidopsis: inplication of the presence of a translesion synthesis mechanism in plants. PLANT CELL 15 9 2042-2057 2003/09
Conference Presentations
Others
Arts and Fieldwork
Patent
Theme to the desired joint research
○Research on anti-cancer drugs targeting cellular responses to DNA damage
Grant-in-Aid for Scientific Research
○「ヌクレオチド除去修復機構解明に向けたケミカルアプローチ」(2019-2021)
○「NER中間体がもたらす新たなDNA損傷生成とその防御応答の解析」(2019-2021)
○「新規DNA修復阻害剤を活用したメカニズム解析と癌治療への応用」(2016-2018)
○「哺乳類細胞に潜在する未知DNA修復反応の解明」(2016-2017)
○「DNA損傷応答ネットワークを標的にした合成致死に基づく癌治療戦略」
○「シスプラチン抵抗性関連因子ＥＲＣＣ１の分解を誘導する新規低分子化合物の解析」(2013-2014)
○「ケミカルバイオロジーを利用したヒトヌクレオチド除去修復機構の解析」(2013-2015)
○「細胞内高次環境におけるヌクレオチド除去修復とその制御のメカニズム」
○「化合物ライブラリーより発見された新規ＤＮＡ修復阻害物質の創薬研究」
○「ヌクレオチド除去修復を阻害する新規化合物を利用したメカニズム解析とその応用」
○「ケミカルバイオロジーを利用したヒトヌクレオチド除去修復機構の解析」(2013-2015)
○「シスプラチン抵抗性関連因子ERCC1の分解を誘導する新規低分子化合物の解析」(2013-2014)
○「ゲノム損傷応答機構におけるCul4-DDB1ユビキチンリガーゼの新機能の解明」(2009-2011)
○「除去修復エンドヌクレアーゼの機能とその欠損による分子病態に関する研究」(1998-)
○「ヒト細胞におけるヌクレオチド除去修復のバックアップ機構に関する研究」(2003-2004)
○「ヌクレオチド除去修復のバックアップ機構に関する研究」(2004-2004)
○「DNA修復関連因子を利用したDNA損傷検出系の開発」(1999-)
○「ヌクレオチド除去修復反応の細胞内調節機構に関する研究」(2000-2004)
○「除去修復エンドヌクレアーゼの機能とその欠損による分子病態の生化学的解析」(1997-)
○「ヌクレオチド除去修復反応を調節する細胞内因子の解析」(1997-)
Competitive research funding,Contribution
Collaborative research,Consignment study
○Analysis of DNA repair mechanisms in human keratinocytes(2006-2010)
Classes (Bachelors)
○Introduction to Graduation Research in Medical and Pharmaceutical Sciences(2023)
○Medical and Pharmaceutical Sciences Researcher Training 2(2023)
○Molecular and Cellular Biology 3(2023)
○Career Plan 1(2023)
○Career Plan 3(2023)
○Introduction to Life Science(2023)
○Carrer Plan 2(2023)
○Basic Health Science 1(2023)
○Introduction to Graduation Research in Pharmacy 1(2023)
○Molecular and Cellular Biology(2023)
○Presentation and Debate (Freshman Seminar II)(2017)
○Molecular and Cellular Biology 3(2017)
○Advanced Molecular Cell Biology(2017)
○Molecular and Cellular Biology(2017)
○Molecular and Cellular Biology(2017)
○Molecular and Cellular Biology(2016)
○Molecular and Cellular Biology(2016)
○Laboratory Rotation 1(2016)
○Molecular and Cellular Biology 3(2016)
○Advanced Molecular Cell Biology(2016)
Classes (Graduate Schools)
○Molecular Biology of Cancer(2017)
○Advanced Course of Biopharmaceutical Drug Discovery(2017)
○Maintenance of Genomic Integrity(2017)
○Advanced Seminar on Biopharmaceutical Sciences(2017)
○Maintenance of Genomic Integrity(2016)