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Researcher Information

last modified:2020/08/25

Assistant Professor Kobayashi, Isao

Mail

Faculty, Affiliation

College and School Educational Field

Division of Natural Sciences, Graduate School of Natural Science and Technology
Course in Aquatic Biology and Environmental Science, School of Biological Science and Technology, College of Science and Engineering

Laboratory

Laboratory of Stem Cell Development  TEL:076-264-6245 FAX:076-264-6245

Academic Background

【Academic background(Doctoral/Master's Degree)】
Nihon University Doctor Graduate School of Veterinary Medicine 2009 Completed
【Academic background(Bachelor's Degree)】
Nihon University Department of Veterinary Medicine 2006
【Degree】
Ph.D.

Career

Kanazawa University Faculty of Natural System, Institute of Science and Engineering Assistant Professor (Specialized in Research)(2015/11/01-)
University of California, San Diego Department of Cellular and Molecular Medicine Assistant Project Scientist(2015/04/01-2015/10/31)
University of California, San Diego Cellular and Molecular Medicine JSPS Postdoctoral Fellow for Research Abroad(2013/04/01-2015/03/31)
University of California, San Diego Cellular and Molecular Medicine JSPS Research Fellow (PD)(2011/10/01-2013/03/31)
Keio University Department of Cell Differentiation, School of Medicine JSPS Research Fellow (PD)(2009/04/01-2011/09/30)

Year & Month of Birth

1980/08

Academic Society

International Society of Experimental Hematology (ISEH)
Japan Society of Developmental Biologists
Japanese Society of Hematology

Award

○Christa Muller-Sieburg Award(2013/08)

Specialities

Stem Cell Biology、Developmental biology、Veterinary medical science、Hematology

Speciality Keywords

Hematopoietic Stem Cell, Zebrafish, Stem Cell Niche, Live Imaging, Blood

Research Themes

Identification and Characterization of Hematopoietic Stem Cell Niche in The Fish Kidney

Identification and Characterization of Hematopoietic Stem Cell Niche in The Fish Kidney The homeostasis of the blood system is maintained by rare populations of multipotent hematopoietic stem cells (HSCs). HSCs are by far the most widely utilized human stem cells in the clinic in order to treat blood-related disorders, such as leukemia. HSCs are maintained by interactions with cells comprising their microenvironmental niche, which is established in the bone marrow in mammals. Despite the similarity of blood cell subsets, HSCs in teleost fish reside in the urinary organ, kidney, representing a very different environment relative to mammals. We will utilize comparative approaches for the study of HSC niches between zebrafish and mammals. Our ultimate goal is to define the required core components that regulate HSC dormancy, proliferation, and differentiation.

Elucidation of Regulatory Mechanisms Underlying Hematopoietic Stem Cell Development

Elucidation of Regulatory Mechanisms Underlying Hematopoietic Stem Cell Development A major goal of regenerative medicine is to instruct tissue-specific stem cells from induced pluripotent stem cells (iPSCs). Despite decades of efforts, however, generation of HSCs from iPSCs in vitro is not currently possible, indicating that an improved understanding of the native mechanisms of embryonic HSC specification is required. The zebrafish is a powerful genetic model for the study of HSCs. Its transparent embryos allow us to visualize the dynamics of developing HSCs using cell-specific transgenic animals. The movie shown here represents migration of angioblasts (green) along the ventral surface of somites (red). Utilizing “live-imaging” approaches in the zebrafish embryo, we will investigate when, where, and how HSC precursors are regulated by their environmental cells to establish HSC fate during embryogenesis.

Books

  •  Isao Kobayashi Zebrafish, Medaka, and Other Small Fishes Springer 2018

Papers

  •  Melatonin suppresses both osteoblast and osteoclast differentiation through repression of epidermal Erk signaling in the zebrafish scale. Jingjing Kobayashi-Sun, Nobuo Suzuki, Atsuhiko Hattori, Masaaki Yamaguchi, Isao Kobayashi* Biochem Biophys Res Commun 530 4 644-650 2020
  •  Uptake of osteoblast-derived extracellular vesicles promotes the differentiation of osteoclasts in the zebrafish scale. Jingjing Kobayashi-Sun, Shiori Yamamori, Mao Kondo, Junpei Kuroda, Mika Ikegame, Nobuo Suzuki, Kei-ichiro Kitamura, Atsuhiko Hattori, Masaaki Yamaguchi, Isao Kobayashi* Commun Biol 3 1 190 2020
  •  Impact of functional studies on exome sequence variant interpretation in early-onset cardiac conduction system diseases. Kenshi Hayashi*, Ryota Teramoto, Akihiro Nomura, Yoshihiro Asano, Manu Beerens, Yasutaka Kurata, Isao Kobayashi, Noboru Fujino, Hiroshi Furusho, Kenji Sakata, Kenji Onoue, David Y Chiang, Tuomas O Kiviniemi, Eva Buys, Patrick Sips, Micah L Burch, Yanbin Zhao, Amy E Kelly, Masanobu Namura, Yoshihito Kita, Taketsugu Tsuchiya, Bunji Kaku, Kotaro Oe, Yuko Takeda, Tetsuo Konno, Masaru Inoue, Takashi Fujita, Takeshi Kato, Akira Funada, Hayato Tada, Akihiko Hodatsu, Chiaki Nakanishi, Yuichiro Sakamoto, Toyonobu Tsuda, Yoji Nagata, Yoshihiro Tanaka, Hirofumi Okada, Keisuke Usuda, Shihe Cui, Yoshihiko Saito, Calum A MacRae, Seiji Takashima, Masakazu Yamagishi, Masa-aki Kawashiri, Masayuki Takamura Cardiovasc Res cvaa010 2020
  •  Dual role of Jam3b in early hematopoietic and vascular development. Isao Kobayashi*, Jingjing Kobayashi-Sun, Yuto Hirakawa, Madoka Ouchi, Koyuki Yasuda, Hiroyasu Kamei, Shigetomo Fukuhara, Masaaki Yamaguchi Development 147 1 2020
  •  Enrichment of hematopoietic stem/progenitor cells in the zebrafish kidney. Isao Kobayashi*, Mao Kondo, Shiori Yamamori, Jingjing Kobayashi-Sun, Makoto Taniguchi, Kaori Kanemaru, Fumihiko Katakura, David Traver Sci Rep 9 1 14205 2019

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  •  Rap1b Promotes Notch-Signal-Mediated Hematopoietic Stem Cell Development by Enhancing Integrin-Mediated Cell Adhesion. Seung-Sik Rho, Isao Kobayashi, Eri Oguri-Nakamura, Koji Ando, Masakazu Fujiwara, Naomi Kamimura, Hiromi Hirata, Atsuo Iida, Yoshiko Iwai, Naoki Mochizuki, Shigetomo Fukuhara* Dev Cell 49 5 681-696 2019
  •  Anteroposterior molecular registries in ectoderm of the echinus rudiment. Shinya Adachi, Iyo Niimi, Yui Sakai, Fuminori Sato, Takuya Minokawa, Makoto Urata, Atsuko Sehara-Fujisawa, Isao Kobayashi, Masaaki Yamaguchi* Dev Dyn 247 12 1297-1307 2018
  •  RANKL, Ephrin-Eph and Wnt10b are key intercellular communication molecules regulating bone remodeling in autologous transplanted goldfish scales. Yuya Tazaki, Kayo Sugitani, Kazuhiro Ogai, Isao Kobayashi, Haruki Kawasaki, Takafumi Aoyama, Nobuo Suzuki, Yoshiaki Tabuchi, Atsuhiko Hattori, Kei-ichiro Kitamura* Comp Biochem Physiol A Mol Integr Physiol 225 46-58 2018
  •  Effects of low-intensity pulsed ultrasound on osteoclasts: Analysis with goldfish scales as a model of bone. Taizo Hanmoto, Yoshiaki Tabuchi, Mika Ikegame, Takashi Kondo, Kei-ichiro Kitamura, Masato Endo, Isao Kobayashi, Hiroyuki Mishima, Toshio Sekiguchi, Makoto Urata, Azusa Seki, Sachiko Yano, Atsuhiko Hattori, Nobuo Suzuki* Biomed Res 38 1 71-77 2017
  •  Effects of hyperglycemia on bone metabolism and bone matrix in goldfish scales. Kei-ichiro Kitamura*, Tadashi Andoh, Wakana Okesaku, Yuya Tazaki, Kazuhiro Ogai, Kayo Sugitani, Isao Kobayashi, Nobuo Suzuki, Wenxi Chen, Mika Ikegame, Atsuhiko Hattori Comp Biochem Physiol A Mol Integr Physiol 203 152-158 2017
  •  Isolation and characterization of hematopoietic stem cells in teleost fish. Isao Kobayashi, Fumihiko Katakura, Tadaaki Moritomo* Dev Comp Immunol 58 86-94 2016
  •  Gata2b is a restricted early regulator of hemogenic endothelium in the zebrafish embryo. Emerald Butko, Martin Distel, Claire Pouget, Bart Weijts, Isao Kobayashi, Kevin Ng, Christian Mosimann, Fabienne E. Poulain, Adam McPherson, Chih-Wen Ni, David L. Stachura, Natasha Del Cid, Raquel Espin-Palazon, Nathan D. Lawson, Richard Dorsky, Wilson K. Clements, David Traver* Development 142 6 1050-1061 2015
  •  The somite: the central organ of the aortic hematopoiesis. Claire Pouget, Isao Kobayashi Medicine/Sciences 31 1 25-27 2015
  •  Jam1a-Jam2a interactions regulate haematopoietic stem cell fate through Notch signalling. Isao Kobayashi, Jingjing Kobayashi-Sun, Albert D. Kim, Claire Pouget, Naonobu Fujita, Toshio Suda, David Traver* Nature 512 7514 319-323 2014
  •  Comparative gene expression analysis of zebrafish and mammals identifies common regulators in hematopoietic stem cells. Isao Kobayashi*, Hiromasa Ono, Tadaaki Moritomo, Koichiro Kano, Teruyuki Nakanishi, Toshio Suda Blood 115 2 e1-9 2010
  •  Kinetics of CD4+ and CD8alpha+ T-cell subsets in graft-versus-host reaction (GVHR) in ginbuna crucian carp Carassius auratus langsdorfii. Yasuhiro Shibasaki, Hideaki Toda, Isao Kobayashi, Tadaaki Moritomo, Teruyuki Nakanishi* Dev Comp Immunol 34 10 1075-1081 2010
  •  Isolation and characterization of endosteal niche cell populations that regulate hematopoietic stem cells. Yuka Nakamura, Fumio Arai*, Hiroko Iwasaki, Kentaro Hosokawa, Isao Kobayashi, Yumiko Gomei, Yoshiko Matsumoto, Hiroki Yoshihara, Toshio Suda Blood 116 9 1422-1432 2010
  •  Molecular cloning and expression analysis of T-bet in ginbuna crucian carp (Carassius auratus langsdorfii). Fumio Takizawa, Kyosuke Araki, Isao Kobayashi, Tadaaki Moritomo, Mitsuru Ototake, Teruyuki Nakanishi* Mol Immunol 45 1 127-136 2008
  •  Characterization of teleost phagocyte NADPH oxidase: Molecular cloning and expression analysis of carp (Cyprinus carpio) phagocyte NADPH oxidase. Masayuki Mayumi, Yoko Takeda, Masataka Hoshiko, Ken Serada, Michihiro Murata, Tadaaki Moritomo*, Fumio Takizawa, Isao Kobayashi, Kyosuke Araki, Teruyuki Nakanishi, Hideki Sumimoto Mol Immunol 45 6 1720-1731 2008
  •  Characterization and localization of side population (SP) cells in zebrafish kidney hematopoietic tissue. Isao Kobayashi, Kazuyuki Saito, Tadaaki Moritomo*, Kyosuke Araki, Fumio Takizawa, Teruyuki Nakanishi Blood 111 3 1131-1137 2008
  •  Long-term hematopoietic reconstitution by transplantation of kidney hematopoietic stem cells in lethally irradiated clonal ginbuna crucian carp (Carassius auratus langsdorfii). Isao Kobayashi*, Sawako Kuniyoshi, Kazuyuki Saito, Tadaaki Moritomo, Tomoko Takahashi, Teruyuki Nakanishi Dev Comp Immunol 32 8 957-965 2008
  •  In vivo characterization of primitive hematopoietic cells in clonal ginbuna crucian carp (Carassius auratus langsdorfii). Isao Kobayashi*, Hiroko Kusakabe, Hideaki Toda, Tadaaki Moritomo, Tomoko Takahashi, Teruyuki Nakanishi Vet Immunol Immunopathol 126 1-2 74-82 2008
  •  Isolation of side population cells from ginbuna carp (Carassius auratus langsdorfii) kidney hematopoietic tissues. Isao Kobayashi, Tadaaki Moritomo*, Mitsuru Ototake, Teruyuki Nakanishi Dev Comp Immunol 31 7 696-707 2007
  •  Demonstration of hematopoietic stem cells in ginbuna carp (Carassius auratus langsdorfii) kidney. Isao Kobayashi, Mayuko Sekiya, Tadaaki Moritomo*, Mitsuru Ototake, Teruyuki Nakanishi Dev Comp Immunol 30 11 1034-1046 2006
  •  Regulation of Hematopoietic Stem Cell Development by Notch Signaling Isao Kobayashi Saiboukougaku 35 1 2016
  •  Jam1a - Jam2a interactions regulate hematopoietic stem cell fate through Notch signaling Isao Kobayashi, David Traver Jikkenigaku 33 1 68-71 2015
  •  Jam1a - Jam2a interactions regulate hematopoietic stem cell fate through Notch signaling Isao Kobayashi, David Traver Life Science Shinchakuronbun Review First Author's  2014
  •  Application of clonal ginbuna for regenerative medicine Tadaaki Moritomo, Isao Kobayashi, Fumihiko Katakura, Takuya Yamaguchi Nihonkessenshiketsugakkaishi 21 1 42-44 2010
  •  Identification of Hematopoietic Stem Cells in teleost fish Isao Kobayashi, Tadaaki Moritomo Ketsueki-shuyouka 58 4 449-454 2009

Conference Presentations

  • Identification of hematopoietic stem cell niches in the zebrafish kidney.(conference:Hematology Meeting)(2020/02)
  • The Sinusoidal Endothelium Functions as a Hematopoietic Niche in the Zebrafish Kidney(conference:ISEH 48th Annual Scientific Meeting)(2019/08)
  • Jam3b regulates hemato-vascular development through the repression of the Erk signaling pathway(conference:ISEH 47th Annual Scientific Meeting)(2018/08)
  • Cell-to-cell interaction between endothelial-hematopoietic precursors and somites regulates developmental specification of hematopoietic stem cells through Notch signaling(conference:International Society of Experimental Hematology)(2013)
  • Hematopoietic stem cells marked by Junctional adhesion molecule 1 (jam1) are localized along bilateral collecting tubules in zebrafish kidney(conference:International Society of Experimental Hematology)(2010)

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  • Comparative gene expression analysis of hematopoietic stem cells between fish and mammals (conference:The 6th Annual Meeting of Hematology for Young Investigators)(2010)
  • Hematopoietic stem cells localize on the surface of renal tubules in zebrafish kidney(conference:The 71st Annual Meeting of the Japanese Society of Hematology)(2009)
  • Characterization of hematopoietic stem cells in adult fish kidney(conference:The 7th Stem Cell Symposium)(2009)
  • Gene expression profile of zebrafish hematopoietic stem cells(conference:The 41st Annual Meeting of Japanese Society of Developmental Biologists)(2008)
  • Characterization and localization of side population (SP) cells in zebrafish kidney hematopoietic tissue(conference:The 40th Annual Meeting of Japanese Society of Developmental Biologists)(2007)

Arts and Fieldwork

Patent

Theme to the desired joint research

Grant-in-Aid for Scientific Research

○「造血幹細胞の形成過程におけるインテグリン由来シグナル伝達経路の役割解明」(2019-2021) 
○「ウロコ骨折モデルによる破骨細胞産生新規カップリング因子の同定と分泌時相の解明」(2018-2020) 
○「背側大動脈における造血幹細胞の発生動態の解析」(2017-2018) 
○「栄養環境センサーを分子基軸とした脊椎側弯症に対する発症・進行予測技術の開発」(2017-2019) 

Classes (Bachelors)

○Developmental Biology B(2019)
○Developmental Biology Lab(2019)
○Guide to Biology and Bioengineering B(2019)
○Basic Biology Lab 2(2019)
○Experiments in Biology(2019)
○Basic Biology Lab 1(2019)
○Experiments in Biology(2018)
○Developmental Biology Lab(2018)
○Guide to Biology and Bioengineering B(2018)
○Experiments in Biology(2017)
○Experiments in Biology(2017)
○Developmental Biology Lab(2017)
○Gene・Genome・Neuron・Time・Life(2016)
○Gene・Genome・Neuron・Time・Life(2016)
○Experiments in Biology(2016)
○Experiments in Biology(2016)
○Developmental Biology Lab(2016)

Classes (Graduate Schools)

○Integrated Systems Biology B(2018)
○Integrated Systems Biology A(2018)
○Integrated Systems Biology A(2017)
○Integrated Systems Biology B(2017)
○Basic Systems Biology B(2016)

International Project

International Students

Lecture themes

Others (Social Activities)

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