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Researcher Information

last modified:2017/05/23

Professor SAKURAI Hiroshi

Mail

Faculty, Affiliation

Faculty of Health Sciences, Institute of Medical, Pharmaceutical and Health Sciences

College and School Educational Field

Division of Health Sciences, Graduate School of Medical Science
Division of Health Sciences,Graduate School of Medical Science
Department of Laboratory Sciences,School of Health Sciences, College of Medical, Pharmaceutical and Health Sciences

Laboratory

laboratory of life sciences TEL:076-265-2588 FAX:076-234-4360

Academic Background

【Academic background(Doctoral/Master's Degree)】
Tokyo Metropolitan University Doctor 1990
【Academic background(Bachelor's Degree)】
Tokyo Metropolitan University 198503

Career

Keio University School of Medicine laboratory of molecular genetics(1990/05/01-1996/03/31)
Kanazawa University Faculty of Medicine(1996/04/01-1998/12/31)
Kanazawa University Faculty of Medicine(1999/01/01-)

Year & Month of Birth

1963/03

Academic Society



Award

Specialities

Molecular biology

Speciality Keywords

Research Themes

Transcriptional regulation by yeast transcription factor GAL11

studies on mechanisms of stress response

Books

  •  Transcription regulation in eukariotes Human Frontier Science Program 1999/09

Papers

  •  A genome-wide analysis of transcriptional effect of Gal11 in Saccharomyces cerevisiae. DNA Research 8 1 1-9 2001/01
  •  Activator-specific requirement for the general transcription factor IIE in yeast. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 261 3 734-739 1999/08
  •  Functional connections between Mediator components and general transcription factors of Saccharomyces cerevisiae. The Journal of Biological Chemistry 275 47 37251-37256 2000/11
  •  Functional Correlation among Gal11, TFIIE, and TFIIH in Saccharomyces cerevisiae. Gal11 and TFIIE cooperatively enhance TFIIH-mediated phosphorylation of RNA polymerase II carboxy-terminal domain sequences. The Journal of Biological Chemistry 273 16 9534-9538 1998/04
  •  Promoter structure-dependent functioning of the general transcription factor IIE in Saccharomyces cerevisiae. The Journal of Biological Chemistry 272 25 15936-15942 1997/06

show all

  •  Transcription initiation mediated by initiator binding protein in Saccharomyces cerevisiae. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 255 1 157-163 1999/02
  •  Yeast Gal11 and Transcription Factor IIE Function Through a Common Pathway in Transcriptional Regulation. The Journal of Biological Chemistry 272 51 32663-32669 1997/12
  •  Artificial recruitment of certain Mediator components affects requirement of basal transcription factor IIE. GENES TO CELLS 8 1 41-51 2003/01
  •  A novel domain of the yeast heat shock factor that regulates its activation function. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 261 3 734-739 2001/09
  •  Identification of a novel class of target genes and a novel type of binding sequence of heat shock transcription factor in Saccharomyces cerevisiae. JOURNAL OF BIOLOGICAL CHEMISTRY 280 12 11911-11919 2005/03
  •  Saccharomyces cerevisiae heat shock transcription factor regulates cell wall remodeling in response to heat shock. Eukaryotic Cell 4 6 1050-1056 2005/06
  •  Phosphorylation of the yeast heat shock transcription factor is implicated in gene-specific activation dependent on the architecture of the heat shock element. MOLECULAR AND CELLULAR BIOLOGY 24 9 3648-3659 2004/05
  •  Stress-induced transcription of the endoplasmic reticulum oxidoreductin gene ERO1 in the yeast Saccharomyces cerevisiae. Takemori, Y., Sakaguchi, A., Matsuda, S., Mizukami, Y. and Sakurai, H. MOLECULAR GENETICS AND GENOMICS 275 1 89-96 2006/01
  •  Mutated yeast heat shock transcription factor activates transcription independently of hyperphosphorylation. Hashikawa, N., Mizukami, Y., Imazu, H. and Sakurai, H. JOURNAL OF BIOLOGICAL CHEMISTRY 281 7 3936-3942 2006/02
  •  The DNA-binding domain of yeast Hsf1 regulates both DNA binding and transcriptional activities. Yamamoto, A. and Sakurai H. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 346 4 1324-1329 2006/08
  •  Different mechanisms are involved in the transcriptional activation by heat shock transcription factor through different types of heat shock elements. Hashikawa, N., Yanamoto, N., and Sakurai, H. JOURNAL OF BIOLOGICAL CHEMISTRY 282 14 10333-10340 2007/04
  •  Interaction between heat shock transcription factors (HSFs) and divergent binding sequences: binding specificities of yeast HSFs and human HSF1. Sakurai, H. and Takemori, Y. JOURNAL OF BIOLOGICAL CHEMISTRY 282 18 13334-13341 2007/05
  •  Role of heat shock transcription factor in the Saccharomyces cerevisiae oxidative stress response. Yamamoto, A., Ueda, J., Yamamoto, N., Hashikawa, N. and Sakurai, H. Eukaryotic Cell 6 8 1373-1379 2007/08
  •  HSF1 activation inhibits rapamycin resistance and TOR signaling in Yeast revealed by combined proteomic and genetic analysis. Bandhakavi S, Xie H, O'Callaghan B, Sakurai H, Kim D, Griffin TJ PLoS One 3 2 e1598 2008/02
  •  Regulation of thermotolerance by stress-induced transcription factors in Saccharomyces cerevisiae. Yamamoto N, Maeda Y, Ikeda A, Sakurai H Eukaryotic Cell 7 5 783-790 2008/05
  •  Differential recognition of heat shock elements by members of the heat shock transcription factor family. Yamamoto N, Takemori Y, Sakurai M, Sugiyama K, Sakurai H FEBS Journal 276 1962-1974 2009/04
  •  Diversity in DNA recognition by heat shock transcription factors (HSFs) from model organisms.  Enoki, Y. and Sakura H.  FEBS LETTERS 585 1293-1298 2011/05
  •  Binding affinities and interactions among different heat shock element types and heat shock factors in rice (Oryza sativa L.). Mittal, D. J., Enoki, Y., Lavania, D., Singh, A., Sakurai, H. and Grover, A.  FEBS Journal 2011/10
  •  Novel aspects of heat shock factors: DNA recogniton, chromatin modulation and gene expression. Sakurai, H. and Enoki, Y.  FEBS Journal 277 4140-4149 2010/10
  •  A unified nomenclature for protein subunits of Mediator complexes linking transcriptional regulators to RNA polymerase II. MOLECULAR CELL 14 553-557 2004/06
  •  Galactose transporters discriminate steric anomers at the cell surface in yeast. Fukasawa T, Sakurai H, Nogi Y, Baruffini E FEMS Yeast Research 9 723-732 2009/06
  •  Carboxy-terminal region of the yeast heat shock factor contains two domains that make transcription independent of the TFIIH protein kinase. Sakurai H., Hashikawa, N., Imazu, H. Fukasawa, T. GENES TO CELLS 8 12 951-961 2003/12
  •  In vitro transcription systems from cultured cells and fat body tissue of the silkworm, Bombyx mori. MOLECULAR BIOTECHNOLOGY 8 79-88 1997/08
  •  DNA-binding and transcriptional activities of human HSF4 containing mutations that associate with congenital and age-related cataracts. Enoki, Y., Mukoda, Y., Furutani, C. and Sakurai, H.  BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE 1802 749-753 2010/06
  •  Mutational analysis of human heat-shock transcription factor 1 reveals a regulatory role for oligomerization in DNA-binding specificity.  Takemori, Y., Enoki, Y., Yamamoto, N., Fukai, Y., Adachi, K. and Sakurai, H. BIOCHEMICAL JOURNAL 424 253-261 2009/12
  •  Regulation of chaperone gene expression by heat shock transcription factor in Saccharomyces cerevisiae: Importance in normal cell growth, stress resistance, and longevity Sakurai, H. and Ota, A.  FEBS LETTERS 2011/09
  •  Stress-induced transcription of regulator of G protein signaling 2 (RGS2) by heat shock transcription factor HSF1. BIOCHIMIE 95 1432-1436 2013/04
  •  Evolutionarily conserved domain of heat hock transcription factor negatively regulates oligomerization and DNA binding. Ota, A., Sawai, M. and Sakurai, H. BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION 1829 9 930-936 2013/09
  •  Dynamic regulation of extracellular signal-regulated kinase (ERK) by protein phosphatase 2A regulatory subunit B56gamma1 in nuclei induces cell migration. PLoS One 8 5 e63727  2013/05
  •  Activation of ERK/IER3/PP2A-B56 positive feedback loop in lung adenocarcinoma by allelic deletion of B56 gene. Ito, T., Ozaki, S., Chanasong, R., Mizutani, Y., Oyama, T., Sakurai, H., Matsumoto, I., Takemura, H. and Kawahara, E. Oncology Reports  35 5 2635-2642 2016/05/01
  •  Heme orientation of cavity mutant hemoglobins (F8His→Gly) in either  or  subunits: Circular dichroism, 1H NMR, and resonance Raman studies.  Nagai, M., Nagai, Y., Aki, Y., Sakurai, H., Mizusawa, N., Ogura, T., Kitagawa, T., Yamamoto, Y. and Nagatomo, S. Chirality  28 8 585-592 2016/08
  •  HSF1 transcriptional activity is modulated by IER5 and PP2A/B55. Ishikawa Y, Kawabata S, Sakurai H. FEBS Letters  589 1150-1155 2015/06
  •  An origin of cooperative oxygen binding of human adult hemoglobin: different roles of the  and  subunits in the 22 tetramer. Nagatomo, S., Nagai, Y., Aki, Y., Sakurai, H., Imai, K., Mizusawa, N., Ogura, T., Kitagawa, T. and Nagai, M. PLoS One  10 e0135080 2015/04
  •  Involvement of propionate side chains of the heme in circular dichroism of myoglobin: Experimental and theoretical analysis. Nagai, M., Kobayashi, C., Nagai, Y., Imai, K., Mizusawa, H., Sakurai, H., Neya, S., Kayanuma, M., Shoji, M. and Nagatomo, S. J. Phys. Chem. B 119 4 1275-1287 2015/01
  •  Heat-induced expression of the immediate-early gene IER5 and its involvement in the proliferation of heat-shocked cells. Ishikawa Y, Sakurai H. FEBS Journal  282 332-340 2015/01
  •  Heat shock transcription factor HSF1 regulates the expression of the Huntingtin-interacting protein HYPK. Sakurai H, Sawai M, Ishikawa Y, Ota A, Kawahara E Biochim. Biophys. Acta 1840 1181-1187 2014/04
  •  The proto-oncogene JUN is a target of the heat shock transcription factor HSF1. Sawai M, Ishikawa Y, Ota A, Sakurai H. FEBS Journal  280 6672-6680 2013/12
  •  Immediate-early response 5 (IER5) interacts with protein phosphatase 2A and regulates the phosphorylation of ribosomal protein S6 kinase and heat shock factor 1. Kawabata S, Ishita Y, Ishikawa Y, Sakurai H. FEBS Letters  589 3679-3685 2015/10

Conference Presentations

  • Heat shock response and MAP kinase regulation by growth arrest and DNA damage-inducible GADD45(2016/11/30)

Arts and Fieldwork

Patent

Theme to the desired joint research

○development of new techniques for molecular biology

Grant-in-Aid for Scientific Research

○「基本転写因子のプロモーター構造特異的な作用機構」(1997-) 
○「転写調節における基本転写因子とプロモーターの特異的相互作用」(1998-) 
○「基本転写因子のプロモーター構造特異的な作用機構」(1998-) 
○「転写調節因子およびプロモーター構造に特異的な転写開始機構」(1999-) 
○「転写開始から伸長反応への移行における転写因子の作用機構」(1999-) 
○「転写開始複合体を形成するタンパク質間の遺伝子特異的な相互作用」(2000-)
○「転写開始複合体を形成するタンパク質間の遺伝子特異的な相互作用」(2000-) 
○「転写開始から伸長反応への移行における転写因子の作用機構」(2000-) 
○「熱ショック転写因子と転写開始複合体の相互作用」(2001-) 
○「熱ショック転写因子による多様な塩基配列の認識と生物間での普遍性」(2007-2008) 
○「ストレス応答における熱ショック転写因子のリン酸化制御」(2004-) 
○「熱ショック転写因子による多様な塩基配列の認識と生物間での普遍性」(2007-) 
○「熱ショック転写因子による遺伝子特異的な転写制御機構」(2009-)
○「熱ショック転写因子による遺伝子特異的な転写制御機構」(2009-2011) 

Classes (Bachelors)

○Biochemistry(2017)
○Experiments in Biochemistry(2017)
○Introduction to Information Technology(2017)
○Genetics(2017)
○Exercise on Recombinant DNA Technology(2017)
○Molecular Biology Lecture and Practice(2017)
○Biochemistry 1(2017)
○Experiments in Life Science(2017)
○Gene Diagnostics(2017)
○Biochemistry(2017)
○Molecular and Cellular Biology(2017)
○Molecular and Cellular Biology(2017)
○Molecular and Cellular Biology(2017)
○Molecular and Cellular Biology(2017)
○Biochemistry(2016)
○Gene Diagnostics(2016)
○Molecular Biology Lecture and Practice(2016)
○Biochemistry 1(2016)
○Exercise on Recombinant DNA Technology(2016)
○Experiments in Life Science(2016)
○Genetics(2016)
○Molecular and Cellular Biology(2016)
○Molecular and Cellular Biology(2016)
○Molecular and Cellular Biology(2016)
○Molecular and Cellular Biology(2016)

Classes (Graduate Schools)

○Molecular Biology of Tumor: Lecture(2017)
○Molecular Biology of Tumor: Seminar(2017)
○An Introduction to Medical Laboratory Sciences:Lec(2017)
○Laboratory Oncology: Research(2017)
○Laboratory Oncology: Seminar(2017)
○Laboratory Oncology: Lecture(2017)
○Advanced Laboratory Oncology: Research(2017)
○Laboratory Oncology: Lecture(2016)
○Advanced Laboratory Oncology: Research(2016)
○Laboratory Oncology: Seminar(2016)
○An Introduction to Medical Laboratory Sciences:Lec(2016)
○Molecular Biology of Tumor: Lecture(2016)
○Molecular Biology of Tumor: Seminar(2016)
○Laboratory Oncology: Research(2016)

International Project

International Students

Lecture themes

Others (Social Activities)

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