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Professor SAKURAI Hiroshi
Faculty, Affiliation
Faculty of Health Sciences, Institute of Medical, Pharmaceutical and Health Sciences
College and School Educational Field
Division of Health Sciences, Graduate School of Medical Science
Division of Health Sciences，Graduate School of Medical Science
Department of Laboratory Sciences，School of Health Sciences, College of Medical, Pharmaceutical and Health Sciences
Laboratory
laboratory of life sciences TEL:076-265-2588 FAX:076-234-4360
Academic Background
【Academic background(Doctoral/Master's Degree)】
Tokyo Metropolitan University Doctor Graduate School, Division of Natural Science 1990
【Academic background(Bachelor's Degree)】
Tokyo Metropolitan University 198503
Career
Keio University School of Medicine laboratory of molecular genetics(1990/05/01-1996/03/31)
Kanazawa University Faculty of Medicine(1996/04/01-1998/12/31)
Kanazawa University Faculty of Medicine(1999/01/01-)
Year & Month of Birth
1963/03
Academic Society
Award
Specialities
Molecular biology
Speciality Keywords
Research Themes
Transcriptional regulation by yeast transcription factor GAL11
studies on mechanisms of stress response
Books
- Transcription regulation in eukariotes Human Frontier Science Program 1999/09
Papers
- A genome-wide analysis of transcriptional effect of Gal11 in Saccharomyces cerevisiae. DNA Research 8 1 1-9 2001/01
- Activator-specific requirement for the general transcription factor IIE in yeast. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 261 3 734-739 1999/08
- Functional connections between Mediator components and general transcription factors of Saccharomyces cerevisiae. The Journal of Biological Chemistry 275 47 37251-37256 2000/11
- Functional Correlation among Gal11, TFIIE, and TFIIH in Saccharomyces cerevisiae. Gal11 and TFIIE cooperatively enhance TFIIH-mediated phosphorylation of RNA polymerase II carboxy-terminal domain sequences. The Journal of Biological Chemistry 273 16 9534-9538 1998/04
- Promoter structure-dependent functioning of the general transcription factor IIE in Saccharomyces cerevisiae. The Journal of Biological Chemistry 272 25 15936-15942 1997/06
- Transcription initiation mediated by initiator binding protein in Saccharomyces cerevisiae. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 255 1 157-163 1999/02
- Yeast Gal11 and Transcription Factor IIE Function Through a Common Pathway in Transcriptional Regulation. The Journal of Biological Chemistry 272 51 32663-32669 1997/12
- Artificial recruitment of certain Mediator components affects requirement of basal transcription factor IIE. GENES TO CELLS 8 1 41-51 2003/01
- A novel domain of the yeast heat shock factor that regulates its activation function. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 261 3 734-739 2001/09
- Identification of a novel class of target genes and a novel type of binding sequence of heat shock transcription factor in Saccharomyces cerevisiae. JOURNAL OF BIOLOGICAL CHEMISTRY 280 12 11911-11919 2005/03
- Saccharomyces cerevisiae heat shock transcription factor regulates cell wall remodeling in response to heat shock. Eukaryotic Cell 4 6 1050-1056 2005/06
- Phosphorylation of the yeast heat shock transcription factor is implicated in gene-specific activation dependent on the architecture of the heat shock element. MOLECULAR AND CELLULAR BIOLOGY 24 9 3648-3659 2004/05
- Stress-induced transcription of the endoplasmic reticulum oxidoreductin gene ERO1 in the yeast Saccharomyces cerevisiae. Takemori, Y., Sakaguchi, A., Matsuda, S., Mizukami, Y. and Sakurai, H. MOLECULAR GENETICS AND GENOMICS 275 1 89-96 2006/01
- Mutated yeast heat shock transcription factor activates transcription independently of hyperphosphorylation. Hashikawa, N., Mizukami, Y., Imazu, H. and Sakurai, H. JOURNAL OF BIOLOGICAL CHEMISTRY 281 7 3936-3942 2006/02
- PP2A adapter protein IER5 induces dephosphorylation and degradation of MDM2, thereby stabilizing p53 T Nakada, M Koga, H Takeuchi, K Doi, H Sugiyama, H Sakurai Cellular Signalling 2025
- The DNA-binding domain of yeast Hsf1 regulates both DNA binding and transcriptional activities. Yamamoto, A. and Sakurai H. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 346 4 1324-1329 2006/08
- Different mechanisms are involved in the transcriptional activation by heat shock transcription factor through different types of heat shock elements. Hashikawa, N., Yanamoto, N., and Sakurai, H. JOURNAL OF BIOLOGICAL CHEMISTRY 282 14 10333-10340 2007/04
- Interaction between heat shock transcription factors (HSFs) and divergent binding sequences: binding specificities of yeast HSFs and human HSF1. Sakurai, H. and Takemori, Y. JOURNAL OF BIOLOGICAL CHEMISTRY 282 18 13334-13341 2007/05
- Role of heat shock transcription factor in the Saccharomyces cerevisiae oxidative stress response. Yamamoto, A., Ueda, J., Yamamoto, N., Hashikawa, N. and Sakurai, H. Eukaryotic Cell 6 8 1373-1379 2007/08
- HSF1 activation inhibits rapamycin resistance and TOR signaling in Yeast revealed by combined proteomic and genetic analysis. Bandhakavi S, Xie H, O'Callaghan B, Sakurai H, Kim D, Griffin TJ PLoS One 3 2 e1598 2008/02
- Regulation of thermotolerance by stress-induced transcription factors in Saccharomyces cerevisiae. Yamamoto N, Maeda Y, Ikeda A, Sakurai H Eukaryotic Cell 7 5 783-790 2008/05
- Differential recognition of heat shock elements by members of the heat shock transcription factor family. Yamamoto N, Takemori Y, Sakurai M, Sugiyama K, Sakurai H FEBS Journal 276 1962-1974 2009/04
- Diversity in DNA recognition by heat shock transcription factors (HSFs) from model organisms. Enoki, Y. and Sakura H. FEBS LETTERS 585 1293-1298 2011/05
- Binding affinities and interactions among different heat shock element types and heat shock factors in rice (Oryza sativa L.). Mittal, D. J., Enoki, Y., Lavania, D., Singh, A., Sakurai, H. and Grover, A. FEBS Journal 2011/10
- Novel aspects of heat shock factors: DNA recogniton, chromatin modulation and gene expression. Sakurai, H. and Enoki, Y. FEBS Journal 277 4140-4149 2010/10
- A unified nomenclature for protein subunits of Mediator complexes linking transcriptional regulators to RNA polymerase II. MOLECULAR CELL 14 553-557 2004/06
- Galactose transporters discriminate steric anomers at the cell surface in yeast. Fukasawa T, Sakurai H, Nogi Y, Baruffini E FEMS Yeast Research 9 723-732 2009/06
- Carboxy-terminal region of the yeast heat shock factor contains two domains that make transcription independent of the TFIIH protein kinase. Sakurai H., Hashikawa, N., Imazu, H. Fukasawa, T. GENES TO CELLS 8 12 951-961 2003/12
- In vitro transcription systems from cultured cells and fat body tissue of the silkworm, Bombyx mori. MOLECULAR BIOTECHNOLOGY 8 79-88 1997/08
- DNA-binding and transcriptional activities of human HSF4 containing mutations that associate with congenital and age-related cataracts. Enoki, Y., Mukoda, Y., Furutani, C. and Sakurai, H. BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE 1802 749-753 2010/06
- Mutational analysis of human heat-shock transcription factor 1 reveals a regulatory role for oligomerization in DNA-binding specificity. Takemori, Y., Enoki, Y., Yamamoto, N., Fukai, Y., Adachi, K. and Sakurai, H. BIOCHEMICAL JOURNAL 424 253-261 2009/12
- Regulation of chaperone gene expression by heat shock transcription factor in Saccharomyces cerevisiae: Importance in normal cell growth, stress resistance, and longevity Sakurai, H. and Ota, A. FEBS LETTERS 2011/09
- Stress-induced transcription of regulator of G protein signaling 2 (RGS2) by heat shock transcription factor HSF1. BIOCHIMIE 95 1432-1436 2013/04
- Evolutionarily conserved domain of heat hock transcription factor negatively regulates oligomerization and DNA binding. Ota, A., Sawai, M. and Sakurai, H. BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION 1829 9 930-936 2013/09
- Dynamic regulation of extracellular signal-regulated kinase (ERK) by protein phosphatase 2A regulatory subunit B56gamma1 in nuclei induces cell migration. PLoS One 8 5 e63727 2013/05
- PP2A and its adapter protein IER5 induce the DNA-binding ability and target gene expression of E2F1 via dephosphorylation at serine 375 Hiroto Takeuchi, Mayuko Koga, Kuriko Doi, Hiroshi Sakurai Biochim Biophys Acta Gene Regul Mech 1866 3 194960 2023/09
- PP2A-B55 and its adapter proteins IER2 and IER5 regulate the activity of RB family proteins and the expression of cell cycle-related genes. Doi, K., Takeuchi, H. and Sakurai, H. FEBS Journal 290 2 745-762 2023/02
- IER family proteins are regulators of protein phosphatase PP2A and modulate the phosphorylation status of CDC25A. Ueda T, Kohama Y, Sakurai H. Cellular Signalling 55 81-89 2019/03
- Regulation of the stability and activity of CDC25A and CDC25B by protein phosphatase PP2A and 14-3-3 binding. Kohama Y, Saito M, Yada M, Sakurai H. Cellular Signalling 54 10-16 2019/02
- TAF7 is a heat-inducible unstable protein and is required for sustained expression of heat shock protein genes. Nagashimada M, Ueda T, Ishita Y, Sakurai H FEBS Journal 285 3215-3224 2018/09
- GADD45 family proteins suppress JNK signaling by targeting MKK7. Ueda T, Kohama Y, Kuge A, Kido E, Sakurai H Archives of Biochemistry and Biophysics 635 1-7 2017/12/01
- Activation of ERK/IER3/PP2A-B56 positive feedback loop in lung adenocarcinoma by allelic deletion of B56 gene. Ito, T., Ozaki, S., Chanasong, R., Mizutani, Y., Oyama, T., Sakurai, H., Matsumoto, I., Takemura, H. and Kawahara, E. Oncology Reports 35 5 2635-2642 2016/05/01
- Heme orientation of cavity mutant hemoglobins (F8His→Gly) in either or subunits: Circular dichroism, 1H NMR, and resonance Raman studies. Nagai, M., Nagai, Y., Aki, Y., Sakurai, H., Mizusawa, N., Ogura, T., Kitagawa, T., Yamamoto, Y. and Nagatomo, S. Chirality 28 8 585-592 2016/08
- HSF1 transcriptional activity is modulated by IER5 and PP2A/B55. Ishikawa Y, Kawabata S, Sakurai H. FEBS Letters 589 1150-1155 2015/06
- An origin of cooperative oxygen binding of human adult hemoglobin: different roles of the and subunits in the 22 tetramer. Nagatomo, S., Nagai, Y., Aki, Y., Sakurai, H., Imai, K., Mizusawa, N., Ogura, T., Kitagawa, T. and Nagai, M. PLoS One 10 e0135080 2015/04
- Involvement of propionate side chains of the heme in circular dichroism of myoglobin: Experimental and theoretical analysis. Nagai, M., Kobayashi, C., Nagai, Y., Imai, K., Mizusawa, H., Sakurai, H., Neya, S., Kayanuma, M., Shoji, M. and Nagatomo, S. J. Phys. Chem. B 119 4 1275-1287 2015/01
- Heat-induced expression of the immediate-early gene IER5 and its involvement in the proliferation of heat-shocked cells. Ishikawa Y, Sakurai H. FEBS Journal 282 332-340 2015/01
- Heat shock transcription factor HSF1 regulates the expression of the Huntingtin-interacting protein HYPK. Sakurai H, Sawai M, Ishikawa Y, Ota A, Kawahara E Biochim. Biophys. Acta 1840 1181-1187 2014/04
- The proto-oncogene JUN is a target of the heat shock transcription factor HSF1. Sawai M, Ishikawa Y, Ota A, Sakurai H. FEBS Journal 280 6672-6680 2013/12
- Immediate-early response 5 (IER5) interacts with protein phosphatase 2A and regulates the phosphorylation of ribosomal protein S6 kinase and heat shock factor 1. Kawabata S, Ishita Y, Ishikawa Y, Sakurai H. FEBS Letters 589 3679-3685 2015/10
Conference Presentations
- Functional regulation of transcription factor E2F1 by phosphorylation(2022/12/01)
- Protein phosphatase PP2A adapter proteins regulate phosphorylation and activity of Rb.(2021/12/02)
- Regulation of cell cycle gene expression by protein phosphatase 2A and its adaptor proteins.(2020/12/02)
- Heat shock response and MAP kinase regulation by growth arrest and DNA damage-inducible GADD45(2016/11/30)
Others
Arts and Fieldwork
Patent
Theme to the desired joint research
○development of new techniques for molecular biology
Grant-in-Aid for Scientific Research
○「基本転写因子のプロモーター構造特異的な作用機構」(1997-)
○「転写調節における基本転写因子とプロモーターの特異的相互作用」(1998-)
○「基本転写因子のプロモーター構造特異的な作用機構」(1998-)
○「転写調節因子およびプロモーター構造に特異的な転写開始機構」(1999-)
○「転写開始から伸長反応への移行における転写因子の作用機構」(1999-)
○「転写開始複合体を形成するタンパク質間の遺伝子特異的な相互作用」(2000-)
○「転写開始複合体を形成するタンパク質間の遺伝子特異的な相互作用」(2000-)
○「転写開始から伸長反応への移行における転写因子の作用機構」(2000-)
○「熱ショック転写因子と転写開始複合体の相互作用」(2001-)
○「熱ショック転写因子による多様な塩基配列の認識と生物間での普遍性」(2007-2008)
○「ストレス応答における熱ショック転写因子のリン酸化制御」(2004-)
○「熱ショック転写因子による多様な塩基配列の認識と生物間での普遍性」(2007-)
○「熱ショック転写因子による遺伝子特異的な転写制御機構」(2009-)
○「運動と非アルコール性脂肪肝炎の改善をつなぐ制御分子機構の解明」(2022-2025)
○「運動と非アルコール性脂肪肝炎の改善をつなぐ制御分子機構の解明」(2022-2025)
○2022「プロテインホスファターゼPP2Aによるシグナル伝達と細胞増殖の制御」(2020-2022)
○「タンパク質ミスフォールディングに注目したうつ病発症メカニズムの解明」(2019-2021)
○「熱ショック転写因子によるシャペロン非依存的恒常性維持機構の解明」(2016-2018)
○「熱ショック転写因子による遺伝子特異的な転写制御機構」(2009-2011)
○「熱ショック転写因子によるシャペロン非依存的恒常性維持機構の解明」(2016-2018)
Competitive research funding,Contribution
Collaborative research,Consignment study
Classes (Bachelors)
○Molecular Biology(2023)
○Gene Diagnostics(2023)
○Biochemistry(2023)
○Gene Diagnostics(2023)
○Experiments in Biochemistry(2023)
○Molecular Biology(2023)
○Biochemistry(2023)
○Biological Chemistry Practice(2023)
○Biochemistry(2023)
○Biochemistry(2023)
○Biological Chemistry Practice(2023)
○Molecular and Cellular Biology(2023)
○Molecular and Cellular Biology(2023)
○Introduction to Data Science(2023)
○Molecular and Cellular Biology(2022)
○Molecular and Cellular Biology(2022)
○Introduction to Data Science(2022)
○Exercise on Recombinant DNA Technology(2022)
○Gene Diagnostics(2022)
○Biochemistry(2022)
○Biochemistry(2022)
○Experiments in Biochemistry(2022)
○Exercise on Recombinant DNA Technology(2022)
○Gene Diagnostics(2022)
○Genetics(2022)
○Molecular Biology Lecture and Practice(2022)
○Experiments in Life Science(2022)
○Experiments in Life Science(2022)
○Biochemistry(2022)
○Introduction to Data Science(2021)
○Molecular and Cellular Biology(2021)
○Molecular and Cellular Biology(2021)
○Gene Diagnostics(2021)
○Exercise on Recombinant DNA Technology(2021)
○Genetics(2021)
○Experiments in Biochemistry(2021)
○Biochemistry(2021)
○Experiments in Life Science(2021)
○Experiments in Life Science(2021)
○Molecular Biology Lecture and Practice(2021)
○Biochemistry(2021)
○Biochemistry(2020)
○Exercise on Recombinant DNA Technology(2020)
○Experiments in Biochemistry(2020)
○Genetics(2020)
○Molecular Biology Lecture and Practice(2020)
○Biochemistry(2020)
○Experiments in Life Science(2020)
○Experiments in Life Science(2020)
○Experiments in Life Science(2020)
○Experiments in Life Science(2020)
○Gene Diagnostics(2020)
○Gene Diagnostics(2020)
○Clinial Practice of Clinical Chemistry(2020)
○Introduction to Information Technology(2020)
○Molecular and Cellular Biology(2020)
○Molecular and Cellular Biology(2020)
○Molecular and Cellular Biology(2020)
Classes (Graduate Schools)
○Advanced Laboratory Oncology: Research(2023)
○Advanced Laboratory Oncology: Research(2023)
○Advanced Laboratory Oncology: Research(2023)
○Advanced Laboratory Oncology: Research(2023)
○Laboratory Oncology: Seminar(2023)
○Laboratory Oncology: Seminar(2023)
○Advanced Laboratory Oncology: Research(2023)
○Laboratory Oncology: Seminar(2023)
○Advanced Laboratory Oncology: Research(2023)
○Advanced Laboratory Oncology: Research(2023)
○Advanced Laboratory Oncology: Research(2023)
○Laboratory Oncology: Seminar(2023)
○Advanced Laboratory Oncology: Research(2023)
○Advanced Laboratory Oncology: Research(2023)
○Advanced Laboratory Oncology: Research(2023)
○Advanced Laboratory Oncology: Research(2023)
○Advanced Laboratory Oncology: Research(2023)
○Laboratory Oncology: Lecture(2023)
○Laboratory Oncology: Lecture(2023)
○Advanced Laboratory Oncology: Research(2023)
○Advanced Laboratory Oncology: Research(2023)
○Advanced Laboratory Oncology: Research(2023)
○Laboratory Oncology: Lecture(2023)
○Advanced Laboratory Oncology: Research(2023)
○Laboratory Oncology: Lecture(2023)
○Advanced Laboratory Oncology: Research(2023)
○Laboratory Rotation I(2023)
○Molecular Biology of Tumor: Seminar(2023)
○Molecular Biology of Tumor: Seminar(2023)
○Laboratory Oncology: Research(2023)
○Laboratory Oncology: Research(2023)
○Laboratory Oncology: Research(2023)
○Laboratory Oncology: Research(2023)
○Laboratory Oncology: Research(2023)
○Laboratory Oncology: Research(2023)
○Molecular Biology of Tumor: Seminar(2023)
○Molecular Biology of Tumor: Seminar(2023)
○Laboratory Oncology: Research(2023)
○Laboratory Oncology: Research(2023)
○Laboratory Oncology: Research(2023)
○Laboratory Oncology: Research(2023)
○Laboratory Oncology: Research(2023)
○Laboratory Oncology: Research(2023)
○Laboratory Rotation Ⅱ(2023)
○Laboratory Rotation Ｉ(2023)
○Laboratory Oncology: Research(2023)
○Laboratory Oncology: Research(2023)
○Molecular Biology of Tumor: Lecture(2023)
○Laboratory Oncology: Research(2023)
○Laboratory Oncology: Research(2023)
○Molecular Biology of Tumor: Lecture(2023)
○An Introduction to Medical Laboratory Sciences:Lec(2023)
○An Introduction to Medical Laboratory Sciences:Lec(2023)
○Laboratory Oncology: Research(2023)
○Laboratory Oncology: Research(2023)
○Laboratory Oncology: Research(2023)
○Molecular Biology of Tumor: Lecture(2023)
○Molecular Biology of Tumor: Lecture(2023)
○Laboratory Oncology: Research(2023)
○Laboratory RotationⅠ(2023)
○Laboratory RotationⅠ(2023)
○Laboratory RotationⅠ(2023)
○Laboratory Rotation I(2023)
○Laboratory Rotation I(2023)
○Laboratory Oncology: Seminar(2022)
○Advanced Laboratory Oncology: Research(2022)
○Advanced Laboratory Oncology: Research(2022)
○Advanced Laboratory Oncology: Research(2022)
○Advanced Laboratory Oncology: Research(2022)
○Laboratory Oncology: Seminar(2022)
○Advanced Laboratory Oncology: Research(2022)
○Laboratory Oncology: Lecture(2022)
○Advanced Laboratory Oncology: Research(2022)
○Advanced Laboratory Oncology: Research(2022)
○Advanced Laboratory Oncology: Research(2022)
○Advanced Laboratory Oncology: Research(2022)
○Advanced Laboratory Oncology: Research(2022)
○Laboratory Oncology: Lecture(2022)
○Advanced Laboratory Oncology: Research(2022)
○Advanced Laboratory Oncology: Research(2022)
○Laboratory Oncology: Research(2022)
○Molecular Biology of Tumor: Seminar(2022)
○Laboratory Oncology: Research(2022)
○Molecular Biology of Tumor: Seminar(2022)
○Laboratory Oncology: Research(2022)
○An Introduction to Medical Laboratory Sciences:Lec(2022)
○Molecular Biology of Tumor: Lecture(2022)
○Introduction to Health Science: Lecture(2022)
○Laboratory Oncology: Research(2022)
○Molecular Biology of Tumor: Lecture(2022)
○Advanced Laboratory Oncology: Research(2021)
○Laboratory Oncology: Seminar(2021)
○Laboratory Oncology: Lecture(2021)
○Introduction to Health Science: Lecture(2021)
○Laboratory Oncology: Research(2021)
○Molecular Biology of Tumor: Seminar(2021)
○Molecular Biology of Tumor: Lecture(2021)
○An Introduction to Medical Laboratory Sciences:Lec(2021)
○Laboratory Oncology: Lecture(2020)
○Advanced Laboratory Oncology: Research(2020)
○Laboratory Oncology: Seminar(2020)
○Introduction to Health Science: Lecture(2020)
○Laboratory Oncology: Research(2020)
○Molecular Biology of Tumor: Seminar(2020)
○Molecular Biology of Tumor: Lecture(2020)
○An Introduction to Medical Laboratory Sciences:Lec(2020)
○Laboratory Oncology: Research(2016)
○Laboratory Oncology: Lecture(2016)
○Advanced Laboratory Oncology: Research(2016)
○Laboratory Oncology: Seminar(2016)
○An Introduction to Medical Laboratory Sciences:Lec(2016)
○Molecular Biology of Tumor: Lecture(2016)
○Molecular Biology of Tumor: Seminar(2016)